Structure and activity of particulate methane monooxygenase arrays in methanotrophs.
Journal
Nature communications
ISSN: 2041-1723
Titre abrégé: Nat Commun
Pays: England
ID NLM: 101528555
Informations de publication
Date de publication:
05 09 2022
05 09 2022
Historique:
received:
10
04
2022
accepted:
16
08
2022
entrez:
5
9
2022
pubmed:
6
9
2022
medline:
9
9
2022
Statut:
epublish
Résumé
Methane-oxidizing bacteria play a central role in greenhouse gas mitigation and have potential applications in biomanufacturing. Their primary metabolic enzyme, particulate methane monooxygenase (pMMO), is housed in copper-induced intracytoplasmic membranes (ICMs), of which the function and biogenesis are not known. We show by serial cryo-focused ion beam (cryoFIB) milling/scanning electron microscope (SEM) volume imaging and lamellae-based cellular cryo-electron tomography (cryoET) that these ICMs are derived from the inner cell membrane. The pMMO trimer, resolved by cryoET and subtomogram averaging to 4.8 Å in the ICM, forms higher-order hexagonal arrays in intact cells. Array formation correlates with increased enzymatic activity, highlighting the importance of studying the enzyme in its native environment. These findings also demonstrate the power of cryoET to structurally characterize native membrane enzymes in the cellular context.
Identifiants
pubmed: 36064719
doi: 10.1038/s41467-022-32752-9
pii: 10.1038/s41467-022-32752-9
pmc: PMC9445010
doi:
Substances chimiques
Minerals
0
Copper
789U1901C5
Oxygenases
EC 1.13.-
methane monooxygenase
EC 1.14.13.25
Methane
OP0UW79H66
Types de publication
Journal Article
Research Support, N.I.H., Extramural
Research Support, Non-U.S. Gov't
Langues
eng
Sous-ensembles de citation
IM
Pagination
5221Subventions
Organisme : NIGMS NIH HHS
ID : R35 GM118035
Pays : United States
Organisme : Biotechnology and Biological Sciences Research Council
ID : BB/S019553/1
Pays : United Kingdom
Organisme : Wellcome Trust
ID : 206422/Z/17/Z
Pays : United Kingdom
Organisme : Biotechnology and Biological Sciences Research Council
ID : BB/S003339/1
Pays : United Kingdom
Organisme : Medical Research Council
Pays : United Kingdom
Informations de copyright
© 2022. The Author(s).
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