Derivation and culturing of neural stem cells from human embryonic brain tissue.

Brain Brain Neoplasms Humans Neural Stem Cells
Cell culture Cell isolation Developmental biology Health sciences Neuroscience Stem cells

Journal

STAR protocols
ISSN: 2666-1667
Titre abrégé: STAR Protoc
Pays: United States
ID NLM: 101769501

Informations de publication

Date de publication:
16 09 2022
Historique:
entrez: 29 8 2022
pubmed: 30 8 2022
medline: 31 8 2022
Statut: epublish

Résumé

Human neural stem cells (hNSCs) are a valuable tool in brain cancer research since they are used as a normal control for multiple assays, mainly pertaining to toxicity. Here, we present a protocol to safely and successfully derive and culture hNSCs

Identifiants

DOI: 10.1016/j.xpro.2022.101628 PMID: 36035806 PMC: PMC9405532
pubmed: 36035806
doi: 10.1016/j.xpro.2022.101628
pii: S2666-1667(22)00508-1
pmc: PMC9405532
pii:
doi:

Types de publication

Journal Article Research Support, Non-U.S. Gov't

Langues

eng

Sous-ensembles de citation

IM

Pagination

101628

Subventions

Organisme : CIHR
Pays : Canada

Informations de copyright

© 2022 The Author(s).

Déclaration de conflit d'intérêts

S.K.S. is a scientific advisor for Century Therapeutics Inc., and her role in the company has been reviewed and is supported by McMaster University. C.V. is a member of the STAR Protocols advisory board.

Références

Nat Rev Genet. 2004 Feb;5(2):136-44
pubmed: 14735124
Stem Cell Res. 2012 Mar;8(2):141-53
pubmed: 22265735
J Neurooncol. 2012 Sep;109(3):457-66
pubmed: 22752853
Oncogene. 2019 Mar;38(10):1702-1716
pubmed: 30348991

Auteurs

Yujin Suk (Y)

Department of Biochemistry and Biomedical Sciences, McMaster University, Hamilton, ON L8S 4L8, Canada; Michael G DeGroote School of Medicine, McMaster University, Hamilton, ON L8S 4L8, Canada. Electronic address: yujin.suk@medportal.ca.

Agata Kieliszek (A)

Department of Biochemistry and Biomedical Sciences, McMaster University, Hamilton, ON L8S 4L8, Canada.

Daniel Mobilio (D)

Department of Biochemistry and Biomedical Sciences, McMaster University, Hamilton, ON L8S 4L8, Canada.

Chitra Venugopal (C)

Department of Surgery, McMaster University, Hamilton, ON L8S 4L8, Canada.

Sheila K Singh (SK)

Department of Biochemistry and Biomedical Sciences, McMaster University, Hamilton, ON L8S 4L8, Canada; Department of Surgery, McMaster University, Hamilton, ON L8S 4L8, Canada. Electronic address: ssingh@mcmaster.ca.

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Classifications MeSH

Recherchemedicale.com › Système nerveux › Système nerveux central › Encéphale › Derivation and culturing of neural stem cells...
Recherchemedicale.com › Maladies du système nerveux › Tumeurs du système nerveux › Tumeurs du système nerveux central › Tumeurs du cerveau › Derivation and culturing of neural stem cells...
Recherchemedicale.com › Eucaryotes › Animaux › Chordés › Vertébrés › Mammifères › Eutheria › Primates › Haplorhini › Catarrhini › Hominidae › Humains › Derivation and culturing of neural stem cells...
Recherchemedicale.com › Cellules › Cellules souches › Cellules souches neurales › Derivation and culturing of neural stem cells...