Label free optical transmission tomography for biosystems: intracellular structures and dynamics.
Journal
Biomedical optics express
ISSN: 2156-7085
Titre abrégé: Biomed Opt Express
Pays: United States
ID NLM: 101540630
Informations de publication
Date de publication:
01 Aug 2022
01 Aug 2022
Historique:
received:
11
01
2022
revised:
23
04
2022
accepted:
05
05
2022
entrez:
29
8
2022
pubmed:
30
8
2022
medline:
30
8
2022
Statut:
epublish
Résumé
There is an increasing need for label free methods that could reveal intracellular structures and dynamics. In this context, we develop a new optical tomography method working in transmission - full-field optical transmission tomography (FF-OTT). The method can measure the forward scattering signals and reveals the time-dependent metabolic signals in living cells. FF-OTT is a common path interferometer taking advantage of the Gouy phase shift - a π phase shift that the light wave experiences around the focus. By modulating the position of the focus one can alter the phase of the scattered light. Demodulation of images with different phases rejects the background and enhances the light from the depth-of-field, thus producing an optical section. We test FF-OTT by imaging single-cell diatoms and ex vivo biological samples. In fresh samples, we show that the intracellular motions create visible intensity fluctuations in FF-OTT so that the method is able to reveal a metabolic dynamic contrast. FF-OTT was found to be an efficient label free technique that can be readily implemented thanks to a robust common-path speckle-free interferometer design using an incoherent light source.
Identifiants
pubmed: 36032580
doi: 10.1364/BOE.453586
pii: 453586
pmc: PMC9408247
doi:
Types de publication
Journal Article
Langues
eng
Pagination
4190-4203Informations de copyright
© 2022 Optica Publishing Group under the terms of the Optica Open Access Publishing Agreement.
Déclaration de conflit d'intérêts
OT: (P), MB: (P), ACB (P), SA: none, VM: none.
Références
Eur J Biochem. 1994 Feb 15;220(1):239-46
pubmed: 7907018
Opt Lett. 1998 Feb 15;23(4):244-6
pubmed: 18084473
Biomed Opt Express. 2016 Aug 29;7(9):3736-3746
pubmed: 27699134
Clin Exp Ophthalmol. 2009 Jan;37(1):100-17
pubmed: 19338608
Light Sci Appl. 2020 Aug 17;9:140
pubmed: 32864115
Nat Methods. 2011 Sep 29;8(10):811-9
pubmed: 21959136
Nat Commun. 2020 Apr 20;11(1):1868
pubmed: 32313067
Microsc Res Tech. 2004 Oct;65(3):159-65
pubmed: 15605417
Proc Natl Acad Sci U S A. 2013 Sep 24;110(39):15555-60
pubmed: 24019504
Opt Lett. 2003 Feb 15;28(4):224-6
pubmed: 12661526
Opt Lett. 2009 May 15;34(10):1576-8
pubmed: 19448826
Biomed Opt Express. 2019 Dec 23;11(1):492-504
pubmed: 32010530
Biomed Opt Express. 2016 Mar 24;7(4):1511-24
pubmed: 27446672
Biomed Opt Express. 2018 Jan 10;9(2):557-568
pubmed: 29552393
Opt Express. 2019 Jul 8;27(14):19562-19572
pubmed: 31503714
Science. 1955 Mar 11;121(3141):345-9
pubmed: 13237991
Nat Rev Cancer. 2002 Apr;2(4):315-9
pubmed: 12001993
Science. 1991 Nov 22;254(5035):1178-81
pubmed: 1957169
Biotechniques. 2001 Nov;31(5):1076-8, 1080, 1082 passim
pubmed: 11730015
Biomed Opt Express. 2016 Oct 07;7(11):4501-4513
pubmed: 27895991
Opt Lett. 2001 Apr 15;26(8):485-7
pubmed: 18040360
Appl Opt. 2002 Feb 1;41(4):805-12
pubmed: 11993929
Nat Methods. 2005 Dec;2(12):920-31
pubmed: 16299477
Nat Commun. 2015 Aug 24;6:8044
pubmed: 26299252