Characterization of a novel thermostable phospholipase C from T. kodakarensis suitable for oil degumming.

Lecithins Phospholipases Phospholipids Soybean Oil / chemistry Type C Phospholipases / genetics
Enzymatic degumming Phosphatidylcholine phospholipase C Thermococcus kodakarensis Thermostable phospholipase C

Journal

Applied microbiology and biotechnology
ISSN: 1432-0614
Titre abrégé: Appl Microbiol Biotechnol
Pays: Germany
ID NLM: 8406612

Informations de publication

Date de publication:
Aug 2022
Historique:
received: 21 02 2022
accepted: 13 07 2022
revised: 09 07 2022
pubmed: 20 7 2022
medline: 30 7 2022
entrez: 19 7 2022
Statut: ppublish

Résumé

The implementation of cleaner technologies that minimize environmental pollution caused by conventional industrial processes is an increasing global trend. Hence, traditionally used chemicals have been replaced by novel enzymatic alternatives in a wide variety of industrial-scale processes. Enzymatic oil degumming, the first step of the oil refining process, exploits the conversion catalyzed by phospholipases to remove vegetable crude oils' phospholipids. This enzymatic method reduces the gums' volume and increases the overall oil yield. A thermostable phospholipase would be highly advantageous for industrial oil degumming as oil treatment at higher temperatures would save energy and increase the recovery of oil by facilitating the mixing and gums removal. A thermostable phosphatidylcholine (PC) (and phosphatidylethanolamine (PE))-specific phospholipase C from Thermococcus kodakarensis (TkPLC) was studied and completely removed PC and PE from crude soybean oil at 80 °C. Due to these characteristics, TkPLC is an interesting promising candidate for industrial-scale enzymatic oil degumming at high temperatures. KEY POINTS: • A thermostable phospholipase C from T. kodakarensis (TkPLC) has been identified. • TkPLC was recombinantly produced in Pichia pastoris and successfully purified. • TkPLC completely hydrolyzed PC and PE in soybean oil degumming assays at 80 °C.

Identifiants

DOI: 10.1007/s00253-022-12081-z PMID: 35854045
pubmed: 35854045
doi: 10.1007/s00253-022-12081-z
pii: 10.1007/s00253-022-12081-z
doi:

Substances chimiques

Lecithins 0
Phospholipids 0
Soybean Oil 8001-22-7
Phospholipases EC 3.1.-
Type C Phospholipases EC 3.1.4.-

Types de publication

Journal Article

Langues

eng

Sous-ensembles de citation

IM

Pagination

5081-5091

Subventions

Organisme : Fondo para la Investigación Científica y Tecnológica
ID : PICT2015-0303
Organisme : Fondo para la Investigación Científica y Tecnológica
ID : PICT 2018-2569
Organisme : Fondo para la Investigación Científica y Tecnológica
ID : PICT2016-4124

Informations de copyright

© 2022. The Author(s), under exclusive licence to Springer-Verlag GmbH Germany, part of Springer Nature.

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Auteurs

Fiorela Marchisio (F)

Instituto de Procesos Biotecnológicos Y Químicos (IPROBYQ), Facultad de Ciencias, Bioquímicas Y Farmacéuticas, Universidad Nacional de Rosario (UNR), CONICET. Mitre 1998, S2000FWF, Rosario, Argentina.

Luisina Di Nardo (L)

Facultad de Ciencias, Bioquímicas Y Farmacéuticas, Instituto de Biología Celular Y Molecular de Rosario (IBR), Universidad Nacional de Rosario (UNR), CONICET. Ocampo Y Esmeralda S/N, 2000, Rosario, Argentina.

Diego Sebastián Val (DS)

Instituto de Procesos Biotecnológicos Y Químicos (IPROBYQ), Facultad de Ciencias, Bioquímicas Y Farmacéuticas, Universidad Nacional de Rosario (UNR), CONICET. Mitre 1998, S2000FWF, Rosario, Argentina.

Sebastián Cerminati (S)

Instituto de Procesos Biotecnológicos Y Químicos (IPROBYQ), Facultad de Ciencias, Bioquímicas Y Farmacéuticas, Universidad Nacional de Rosario (UNR), CONICET. Mitre 1998, S2000FWF, Rosario, Argentina.

Martín Espariz (M)

Instituto de Procesos Biotecnológicos Y Químicos (IPROBYQ), Facultad de Ciencias, Bioquímicas Y Farmacéuticas, Universidad Nacional de Rosario (UNR), CONICET. Mitre 1998, S2000FWF, Rosario, Argentina.
Área Estadística Y Procesamiento de Datos, Departamento de Matemática Y Estadística, Facultad de Ciencias Bioquímicas Y Farmacéuticas, Universidad Nacional de Rosario, Rosario, Argentina.

Rodolfo Maximiliano Rasia (RM)

Facultad de Ciencias, Bioquímicas Y Farmacéuticas, Instituto de Biología Celular Y Molecular de Rosario (IBR), Universidad Nacional de Rosario (UNR), CONICET. Ocampo Y Esmeralda S/N, 2000, Rosario, Argentina.
Plataforma Argentina de Biología Estructural Y Metabolómica (PLABEM - CONICET), Ocampo y Esmeralda s/n, 2000, Rosario, Argentina.

Hugo Gabriel Menzella (HG)

Instituto de Procesos Biotecnológicos Y Químicos (IPROBYQ), Facultad de Ciencias, Bioquímicas Y Farmacéuticas, Universidad Nacional de Rosario (UNR), CONICET. Mitre 1998, S2000FWF, Rosario, Argentina.

María Eugenia Castelli (ME)

Instituto de Procesos Biotecnológicos Y Químicos (IPROBYQ), Facultad de Ciencias, Bioquímicas Y Farmacéuticas, Universidad Nacional de Rosario (UNR), CONICET. Mitre 1998, S2000FWF, Rosario, Argentina. castelli@iprobyq-conicet.gob.ar.

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Classifications MeSH

Recherchemedicale.com › Mélanges complexes › Produits biologiques › Préparations à base de plantes › Lécithines › Characterization of a novel thermostable...
Recherchemedicale.com › Enzymes et coenzymes › Enzymes › Hydrolases › Esterases › Phosphodiesterases › Phospholipases › Characterization of a novel thermostable...
Recherchemedicale.com › Lipides › Lipides membranaires › Phospholipides › Characterization of a novel thermostable...
Recherchemedicale.com › Aliments et boissons › Aliments › Matières grasses alimentaires › Matières grasses alimentaires insaturées › Huile de soja › Characterization of a novel thermostable...
Recherchemedicale.com › Enzymes et coenzymes › Enzymes › Hydrolases › Esterases › Phosphodiesterases › Phospholipases › Type C Phospholipases › Characterization of a novel thermostable...