Effects of end-stage osteoarthritis on markers of skeletal muscle Long INterspersed Element-1 activity.
LINE-1
Osteoarthritis
STING
Skeletal muscle
Journal
BMC research notes
ISSN: 1756-0500
Titre abrégé: BMC Res Notes
Pays: England
ID NLM: 101462768
Informations de publication
Date de publication:
07 Jul 2022
07 Jul 2022
Historique:
received:
24
09
2021
accepted:
15
06
2022
entrez:
7
7
2022
pubmed:
8
7
2022
medline:
12
7
2022
Statut:
epublish
Résumé
Long INterspersed Element-1 (L1) is an autonomous transposable element in the genome. L1 transcripts that are not reverse transcribed back into the genome can accumulate in the cytoplasm and activate an inflammatory response via the cyclic GMP-AMP (cGAS)-STING pathway. We examined skeletal muscle L1 markers as well as STING protein levels in 10 older individuals (63 ± 11 y, BMI = 30.2 ± 6.8 kg/m L1 mRNA was higher in CTL and SX compared to Y (p < 0.001 and p = 0.001, respectively). Protein expression was higher in SX versus Y for ORF1p (p = 0.002) and STING (p = 0.022). While these data are preliminary due to limited n-sizes and the lack of a BMI-matched younger control group, higher L1 mRNA expression, ORF1p and STING protein are evident in older versus younger adults. More research is needed to determine whether cGAS-STING signaling contributes to heightened muscle inflammation during aging and/or OA.
Identifiants
pubmed: 35799274
doi: 10.1186/s13104-022-06113-0
pii: 10.1186/s13104-022-06113-0
pmc: PMC9264706
doi:
Substances chimiques
Biomarkers
0
RNA, Messenger
0
Nucleotidyltransferases
EC 2.7.7.-
Types de publication
Journal Article
Langues
eng
Sous-ensembles de citation
IM
Pagination
245Subventions
Organisme : NICHD NIH HHS
ID : T32 HD071866
Pays : United States
Organisme : NIH HHS
ID : T32HD071866
Pays : United States
Organisme : NIH HHS
ID : R01HD084124
Pays : United States
Informations de copyright
© 2022. The Author(s).
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