Development of an osmoprotective microemulsion as a therapeutic platform for ocular surface protection.

Hyperosmolar model In vivo tolerance Ocular drug delivery Ophthalmic microemulsions Osmoprotection Stability

Journal

International journal of pharmaceutics
ISSN: 1873-3476
Titre abrégé: Int J Pharm
Pays: Netherlands
ID NLM: 7804127

Informations de publication

Date de publication:
25 Jul 2022
Historique:
received: 27 03 2022
revised: 03 06 2022
accepted: 19 06 2022
pubmed: 26 6 2022
medline: 14 7 2022
entrez: 25 6 2022
Statut: ppublish

Résumé

Self-emulsified osmoprotective ophthalmic microemulsions (O/A) were prepared by combining betaine/leucine, clusterin/oleanolic acid, and hyaluronic acid or Dextran. The microemulsions contained an internal oily phase (1.2%), an external aqueous phase (96.3%), cosolvents (1%), and surfactants (1.5%). Physicochemical characterization and in vivo and in vitro tolerance were analyzed. The formulations' osmoprotective in vitro activity was assayed in a hyperosmolar model in human corneal cells. Average internal phase sizes were 16-26 nm for the microemulsions including Dextran. Addition of hyaluronic acid increased the size range (25-39 nm). Addition of osmoprotectants did not change nanodroplet size. The formulations were isotonic (280-290 mOsm/L) with neutral pH (≈7) and zeta potential (-10 to 0 mV), low surface tension (≈35-40mN·m

Identifiants

pubmed: 35752388
pii: S0378-5173(22)00503-8
doi: 10.1016/j.ijpharm.2022.121948
pii:
doi:

Substances chimiques

Dextrans 0
Emulsions 0
Surface-Active Agents 0
Hyaluronic Acid 9004-61-9

Types de publication

Journal Article

Langues

eng

Sous-ensembles de citation

IM

Pagination

121948

Informations de copyright

Copyright © 2022. Published by Elsevier B.V.

Auteurs

J J López-Cano (JJ)

Ophthalmology Innovation, Therapy and Pharmaceutical Development (InnOftal) Research Group, UCM 920415, Department of Pharmaceutics and Food Technology, Faculty of Pharmacy (UCM), Plaza Ramón y Cajal s/n, Madrid 28040, Spain; National Ocular Pathology Network (OFTARED), Carlos III Health Institute, San Carlos Clinical Hospital Health Research Institute (IdISSC), Madrid 28040, Spain.

M A González-Cela-Casamayor (MA)

Ophthalmology Innovation, Therapy and Pharmaceutical Development (InnOftal) Research Group, UCM 920415, Department of Pharmaceutics and Food Technology, Faculty of Pharmacy (UCM), Plaza Ramón y Cajal s/n, Madrid 28040, Spain; National Ocular Pathology Network (OFTARED), Carlos III Health Institute, San Carlos Clinical Hospital Health Research Institute (IdISSC), Madrid 28040, Spain.

V Andrés-Guerrero (V)

Ophthalmology Innovation, Therapy and Pharmaceutical Development (InnOftal) Research Group, UCM 920415, Department of Pharmaceutics and Food Technology, Faculty of Pharmacy (UCM), Plaza Ramón y Cajal s/n, Madrid 28040, Spain; National Ocular Pathology Network (OFTARED), Carlos III Health Institute, San Carlos Clinical Hospital Health Research Institute (IdISSC), Madrid 28040, Spain.

M Vicario-de-la-Torre (M)

Ophthalmology Innovation, Therapy and Pharmaceutical Development (InnOftal) Research Group, UCM 920415, Department of Pharmaceutics and Food Technology, Faculty of Pharmacy (UCM), Plaza Ramón y Cajal s/n, Madrid 28040, Spain; National Ocular Pathology Network (OFTARED), Carlos III Health Institute, San Carlos Clinical Hospital Health Research Institute (IdISSC), Madrid 28040, Spain.

J M Benítez Del Castillo (JM)

National Ocular Pathology Network (OFTARED), Carlos III Health Institute, San Carlos Clinical Hospital Health Research Institute (IdISSC), Madrid 28040, Spain; Ocular Surface and Inflammation Unit, Ophthalmology Department, San Carlos Clinical Hospital Health Research Institute (IdISSC), Madrid 28040, Spain.

R Herrero-Vanrell (R)

Ophthalmology Innovation, Therapy and Pharmaceutical Development (InnOftal) Research Group, UCM 920415, Department of Pharmaceutics and Food Technology, Faculty of Pharmacy (UCM), Plaza Ramón y Cajal s/n, Madrid 28040, Spain; National Ocular Pathology Network (OFTARED), Carlos III Health Institute, San Carlos Clinical Hospital Health Research Institute (IdISSC), Madrid 28040, Spain. Electronic address: rociohv@ucm.es.

I T Molina-Martínez (IT)

Ophthalmology Innovation, Therapy and Pharmaceutical Development (InnOftal) Research Group, UCM 920415, Department of Pharmaceutics and Food Technology, Faculty of Pharmacy (UCM), Plaza Ramón y Cajal s/n, Madrid 28040, Spain; National Ocular Pathology Network (OFTARED), Carlos III Health Institute, San Carlos Clinical Hospital Health Research Institute (IdISSC), Madrid 28040, Spain. Electronic address: iremm@ucm.es.

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Classifications MeSH