Identification of LOC101927355 as a Novel Biomarker for Preeclampsia.

RNA-Seq cellular localization lncRNAs placenta

Journal

Biomedicines
ISSN: 2227-9059
Titre abrégé: Biomedicines
Pays: Switzerland
ID NLM: 101691304

Informations de publication

Date de publication:
27 May 2022
Historique:
received: 25 04 2022
revised: 23 05 2022
accepted: 25 05 2022
entrez: 24 6 2022
pubmed: 25 6 2022
medline: 25 6 2022
Statut: epublish

Résumé

Preeclampsia, a disorder with a heterogeneous physiopathology, can be attributed to maternal, fetal, and/or placental factors. Long non-coding RNAs (lncRNAs) refer to a class of non-coding RNAs, the essential regulators of biological processes; their differential expression has been associated with the pathogenesis of multiple diseases. The study aimed to identify lncRNAs, expressed in the placentas and plasma of patients who presented with preeclampsia, as potential putative biomarkers of the disease. In silico analysis was performed to determine lncRNAs differentially expressed in the placentas of patients with preeclampsia, using a previously published RNA-Seq dataset. Seven placentas and maternal plasma samples collected at delivery from preterm preeclamptic patients (≤37 gestational weeks of gestation), and controls were used to validate the expression of lncRNAs by qRT-PCR. Six lncRNAs were validated and differentially expressed (p < 0.05) in the preeclampsia and control placentas: UCA1 and HCG4 were found upregulated, and LOC101927355, LINC00551, PART1, and NRAD1 downregulated. Two of these lncRNAs, HCG4 and LOC101927355, were also detected in maternal plasma, the latter showing a significant decrease (p = 0.03) in preeclamptic patients compared to the control group. In silico analyses showed the cytoplasmic location of LOC101927355, which suggests a role in post-transcriptional gene regulation. The detection of LOC101927355 in the placenta and plasma opens new possibilities for understanding the pathogenesis of preeclampsia and for its potential use as a biomarker.

Identifiants

DOI: 10.3390/biomedicines10061253 PMID: 35740273 PMC: PMC9219905
pubmed: 35740273
pii: biomedicines10061253
doi: 10.3390/biomedicines10061253
pmc: PMC9219905
pii:
doi:

Types de publication

Journal Article

Langues

eng

Subventions

Organisme : ANID/CONICYT-FONDECYT Postdoctorado
ID : 3140038
Organisme : ANID/CONICYT-FONDECYT de Iniciación
ID : 11190998
Organisme : ANID/CONICYT-FONDECYT de Iniciación
ID : 11181249
Organisme : ANID/CONICYT- FONDECYT Regular
ID : 1201851
Organisme : ANID/CONICYT-FONDECYT Postdoctorado
ID : 3160622
Organisme : ANID Basal funding for Scientific and Technological Center of Excellence, IMPACT
ID : FB210024

Références

Bioinformatics. 2015 Jan 15;31(2):166-9
pubmed: 25260700
Cancer Manag Res. 2020 Aug 24;12:7753-7760
pubmed: 32922076
PLoS One. 2016 Jun 09;11(6):e0157022
pubmed: 27280887
FEBS Lett. 2008 Jun 11;582(13):1919-27
pubmed: 18501714
BMC Med Genomics. 2019 Jun 27;12(1):91
pubmed: 31248403
Int J Mol Sci. 2022 Mar 01;23(5):
pubmed: 35269870
J Cell Physiol. 2020 Oct;235(10):7410-7419
pubmed: 32067230
Genomics. 2020 May;112(3):2583-2589
pubmed: 32068122
FASEB J. 2019 Nov;33(11):12047-12059
pubmed: 31574234
PLoS One. 2013 Nov 28;8(11):e81437
pubmed: 24312300
Onco Targets Ther. 2020 Oct 14;13:10409-10416
pubmed: 33116620
Biochim Biophys Acta. 2016 Jan;1859(1):3-15
pubmed: 26477492
Brief Bioinform. 2022 Jan 17;23(1):
pubmed: 34498677
Noncoding RNA Res. 2022 Feb 25;7(2):66-70
pubmed: 35310927
Onco Targets Ther. 2020 Nov 09;13:11459-11470
pubmed: 33204101
Genes Dev. 2016 Jan 1;30(1):34-51
pubmed: 26701265
Genome Biol. 2014;15(12):550
pubmed: 25516281
Cancers (Basel). 2021 May 27;13(11):
pubmed: 34072264
Biomed Res Int. 2019 Apr 14;2019:5437621
pubmed: 31111058
Database (Oxford). 2018 Jan 1;2018:1-6
pubmed: 30219837
Acta Biochim Biophys Sin (Shanghai). 2020 Oct 19;52(10):1120-1130
pubmed: 33085763
Cancer Biol Ther. 2019;20(5):583-592
pubmed: 30739532
BMC Cancer. 2022 Jan 31;22(1):124
pubmed: 35100978
Bioinformatics. 2021 Feb 25;:
pubmed: 33630066
Cell Death Differ. 2020 Jan;27(1):363-378
pubmed: 31197235
Genes (Basel). 2021 Jun 25;12(7):
pubmed: 34201957
Sci Rep. 2018 Nov 6;8(1):16385
pubmed: 30401954
Obstet Gynecol. 2020 Jun;135(6):e237-e260
pubmed: 32443079
Trends Mol Med. 2018 Mar;24(3):257-277
pubmed: 29449148
Am J Physiol Endocrinol Metab. 2013 Apr 15;304(8):E836-43
pubmed: 23443922
Bioinformatics. 2013 Jan 1;29(1):15-21
pubmed: 23104886
Biol Chem. 2018 Mar 28;399(4):387-395
pubmed: 29261512
Clin Chim Acta. 2016 Dec 1;463:193-199
pubmed: 27816668
Cell Biosci. 2019 Sep 23;9:78
pubmed: 31572567
Trends Biochem Sci. 2016 Sep;41(9):761-772
pubmed: 27499234
J Clin Med. 2020 Jul 15;9(7):
pubmed: 32679789
Sci Rep. 2020 Oct 7;10(1):16729
pubmed: 33028966
Nat Rev Mol Cell Biol. 2018 Mar;19(3):143-157
pubmed: 29138516
J Cell Physiol. 2021 Feb;236(2):1418-1431
pubmed: 32677057
J Cell Biochem. 2018 Jan;119(1):607-615
pubmed: 28618048
Lancet. 2010 Aug 21;376(9741):631-44
pubmed: 20598363
Curr Drug Targets. 2017;18(10):1165-1170
pubmed: 28382860
Science. 2017 Jan 6;355(6320):
pubmed: 27980086
Mol Med Rep. 2019 Jun;19(6):4806-4818
pubmed: 31059106
Int J Clin Exp Med. 2015 Jul 15;8(7):11824-30
pubmed: 26380024

Auteurs

Reyna Peñailillo (R)

Laboratory of Reproductive Biology, Center for Biomedical Research and Innovation (CIIB), Universidad de los Andes, Santiago 7620001, Chile.
ORCID: 0000-0001-5300-1208

Lara J Monteiro (LJ)

Laboratory of Reproductive Biology, Center for Biomedical Research and Innovation (CIIB), Universidad de los Andes, Santiago 7620001, Chile.
Faculty of Medicine, Universidad de los Andes, Santiago 7620001, Chile.
IMPACT, Center of Interventional Medicine for Precision and Advanced Cellular Therapy, Santiago 7620001, Chile.

Stephanie Acuña-Gallardo (S)

Laboratory of Reproductive Biology, Center for Biomedical Research and Innovation (CIIB), Universidad de los Andes, Santiago 7620001, Chile.
Faculty of Medicine, Universidad de los Andes, Santiago 7620001, Chile.

Felipe García (F)

Laboratory of Reproductive Biology, Center for Biomedical Research and Innovation (CIIB), Universidad de los Andes, Santiago 7620001, Chile.

Victoria Velásquez (V)

Laboratory of Reproductive Biology, Center for Biomedical Research and Innovation (CIIB), Universidad de los Andes, Santiago 7620001, Chile.

Paula Correa (P)

Faculty of Medicine, Universidad de los Andes, Santiago 7620001, Chile.

Pilar Díaz (P)

Faculty of Medicine, Universidad de los Andes, Santiago 7620001, Chile.
Department of Obstetrics and Gynecology, Clínica Dávila, Santiago 7620001, Chile.
ORCID: 0000-0003-0797-7941

Patricia P Valdebenito (PP)

IMPACT, Center of Interventional Medicine for Precision and Advanced Cellular Therapy, Santiago 7620001, Chile.

Cristina Navarro (C)

Departamento de Ciencias Biológicas, Facultad de Ciencias de la Vida, Universidad Andres Bello, Santiago 8370186, Chile.

Roberto Romero (R)

Perinatology Research Branch, Division of Obstetrics and Maternal-Fetal Medicine, Division of Intramural Research, Eunice Kennedy Shriver National Institute of Child Health and Human Development, National Institutes of Health, US Department of Health and Human Services, Bethesda, MD 20892, and Detroit, MI 48201, USA.
Department of Obstetrics and Gynecology, University of Michigan, Ann Arbor, MI 48109, USA.
Department of Epidemiology and Biostatistics, Michigan State University, East Lansing, MI 48824, USA.
Center for Molecular Obstetrics and Genetics, Wayne State University, Detroit, MI 48201, USA.
Detroit Medical Center, Detroit, MI 48201, USA.

Mario Sánchez (M)

Molecular Biology and Bioinformatics Lab, Program in Molecular Biology and Bioinformatics, Center for Biomedical Research and Innovation (CIIB), Universidad de los Andes, Santiago 7620001, Chile.
ORCID: 0000-0002-5991-9930

Sebastián E Illanes (SE)

Laboratory of Reproductive Biology, Center for Biomedical Research and Innovation (CIIB), Universidad de los Andes, Santiago 7620001, Chile.
Faculty of Medicine, Universidad de los Andes, Santiago 7620001, Chile.
IMPACT, Center of Interventional Medicine for Precision and Advanced Cellular Therapy, Santiago 7620001, Chile.
Department of Obstetrics and Gynecology, Clínica Dávila, Santiago 7620001, Chile.

Gino Nardocci (G)

Faculty of Medicine, Universidad de los Andes, Santiago 7620001, Chile.
IMPACT, Center of Interventional Medicine for Precision and Advanced Cellular Therapy, Santiago 7620001, Chile.
Molecular Biology and Bioinformatics Lab, Program in Molecular Biology and Bioinformatics, Center for Biomedical Research and Innovation (CIIB), Universidad de los Andes, Santiago 7620001, Chile.
ORCID: 0000-0002-2342-6480

Classifications MeSH