Single strand gap repair: The presynaptic phase plays a pivotal role in modulating lesion tolerance pathways.
Journal
PLoS genetics
ISSN: 1553-7404
Titre abrégé: PLoS Genet
Pays: United States
ID NLM: 101239074
Informations de publication
Date de publication:
06 2022
06 2022
Historique:
received:
07
09
2021
accepted:
05
05
2022
revised:
16
06
2022
pubmed:
3
6
2022
medline:
22
6
2022
entrez:
2
6
2022
Statut:
epublish
Résumé
During replication, the presence of unrepaired lesions results in the formation of single stranded DNA (ssDNA) gaps that need to be repaired to preserve genome integrity and cell survival. All organisms have evolved two major lesion tolerance pathways to continue replication: Translesion Synthesis (TLS), potentially mutagenic, and Homology Directed Gap Repair (HDGR), that relies on homologous recombination. In Escherichia coli, the RecF pathway repairs such ssDNA gaps by processing them to produce a recombinogenic RecA nucleofilament during the presynaptic phase. In this study, we show that the presynaptic phase is crucial for modulating lesion tolerance pathways since the competition between TLS and HDGR occurs at this stage. Impairing either the extension of the ssDNA gap (mediated by the nuclease RecJ and the helicase RecQ) or the loading of RecA (mediated by RecFOR) leads to a decrease in HDGR and a concomitant increase in TLS. Hence, we conclude that defects in the presynaptic phase delay the formation of the D-loop and increase the time window allowed for TLS. In contrast, we show that a defect in the postsynaptic phase that impairs HDGR does not lead to an increase in TLS. Unexpectedly, we also reveal a strong genetic interaction between recF and recJ genes, that results in a recA deficient-like phenotype in which HDGR is almost completely abolished.
Identifiants
pubmed: 35653392
doi: 10.1371/journal.pgen.1010238
pii: PGENETICS-D-21-01206
pmc: PMC9203016
doi:
Substances chimiques
DNA, Bacterial
0
DNA, Single-Stranded
0
Escherichia coli Proteins
0
Rec A Recombinases
EC 2.7.7.-
Types de publication
Journal Article
Research Support, Non-U.S. Gov't
Langues
eng
Sous-ensembles de citation
IM
Pagination
e1010238Déclaration de conflit d'intérêts
The authors have declared that no competing interests exist.
Références
Int J Biol Sci. 2008 Sep 23;4(6):338-44
pubmed: 18825275
Proc Natl Acad Sci U S A. 2011 Nov 29;108(48):19311-6
pubmed: 22084087
EMBO J. 2006 Dec 13;25(24):5754-63
pubmed: 17139245
Mol Cell Oncol. 2014 Oct 29;1(2):e957039
pubmed: 27308329
Biochimie. 1991 Feb-Mar;73(2-3):313-20
pubmed: 1883889
J Biol Chem. 2009 Jan 30;284(5):3264-3272
pubmed: 18986990
Nucleic Acids Res. 2019 Apr 8;47(6):2946-2965
pubmed: 30657965
Nature. 1974 May 24;249(455):348-9
pubmed: 4601824
Proc Natl Acad Sci U S A. 2014 Dec 2;111(48):E5133-42
pubmed: 25411316
Genes Dev. 2009 May 15;23(10):1234-45
pubmed: 19451222
Prog Biophys Mol Biol. 2017 Aug;127:111-118
pubmed: 27838363
J Biol Chem. 2004 Jan 30;279(5):3492-6
pubmed: 14625283
Microbiol Rev. 1988 Mar;52(1):1-28
pubmed: 3280962
Bioessays. 1993 May;15(5):355-8
pubmed: 8393667
J Bacteriol. 1991 Sep;173(17):5414-8
pubmed: 1653210
Genetics. 1998 May;149(1):7-16
pubmed: 9584082
Proc Natl Acad Sci U S A. 1998 Jun 9;95(12):6909-14
pubmed: 9618512
Mol Gen Genet. 1995 Jan 20;246(2):254-8
pubmed: 7862097
Trends Biochem Sci. 2016 Jun;41(6):491-507
pubmed: 27156117
Mol Gen Genet. 1999 Oct;262(3):543-51
pubmed: 10589843
Sci Rep. 2017 Sep 19;7(1):11876
pubmed: 28928411
Nucleic Acids Res. 2012 Oct;40(18):9036-43
pubmed: 22798494
Annu Rev Genet. 2000;34:479-497
pubmed: 11092836
DNA Repair (Amst). 2011 Mar 7;10(3):260-70
pubmed: 21145792
Proc Natl Acad Sci U S A. 1997 Apr 15;94(8):3714-9
pubmed: 9108043
Elife. 2016 Apr 08;5:e14294
pubmed: 27058167
Mol Syst Biol. 2006;2:2006.0008
pubmed: 16738554
Curr Genet. 2018 Dec;64(6):1189-1196
pubmed: 29700578
Nature. 2001 Jan 18;409(6818):366-70
pubmed: 11201748
Nucleic Acids Res. 2006 Feb 18;34(4):1084-91
pubmed: 16488881
Sci Adv. 2016 Nov 18;2(11):e1601605
pubmed: 28090586
J Mol Biol. 1968 Jan 28;31(2):291-304
pubmed: 4865486
EMBO J. 2000 Nov 15;19(22):6259-65
pubmed: 11080171
Mol Cell. 2006 Jan 6;21(1):15-27
pubmed: 16387650
Cell. 1993 Oct 22;75(2):341-50
pubmed: 8402917
Biochem Biophys Res Commun. 1992 May 15;184(3):1496-503
pubmed: 1590807
Mutat Res. 1994 Jul;315(1):1-9
pubmed: 7517004
J Biol Chem. 1988 Dec 15;263(35):18946-52
pubmed: 3058691
J Biol Chem. 1994 Nov 25;269(47):30005-13
pubmed: 7962001
Nature. 2006 Feb 2;439(7076):557-62
pubmed: 16452972
Mol Cell. 2003 May;11(5):1337-47
pubmed: 12769856
Proc Natl Acad Sci U S A. 1992 Jun 15;89(12):5452-6
pubmed: 1608954
J Bacteriol. 1987 Oct;169(10):4559-64
pubmed: 3308845
J Bacteriol. 2005 Oct;187(20):6953-61
pubmed: 16199565
Oncogene. 2002 Dec 16;21(58):8957-66
pubmed: 12483512
Cold Spring Harb Perspect Biol. 2013 Mar 01;5(3):a012708
pubmed: 23457261
Proc Natl Acad Sci U S A. 1993 May 1;90(9):3875-9
pubmed: 8483906
J Biol Chem. 2006 Sep 29;281(39):28811-21
pubmed: 16895921
Proc Natl Acad Sci U S A. 2006 Jun 13;103(24):9154-9
pubmed: 16754873
PLoS Genet. 2005 Aug;1(2):e15
pubmed: 16132081
Proc Natl Acad Sci U S A. 2014 Apr 15;111(15):5526-31
pubmed: 24706928
J Bacteriol. 1987 Apr;169(4):1731-6
pubmed: 3031020
PLoS Genet. 2015 Dec 29;11(12):e1005757
pubmed: 26713761
Nucleic Acids Res. 2018 May 4;46(8):4004-4012
pubmed: 29529312
Proc Natl Acad Sci U S A. 2000 Jun 6;97(12):6640-5
pubmed: 10829079
J Bacteriol. 2002 Aug;184(16):4449-54
pubmed: 12142415
Mutat Res. 1993 Aug;294(2):157-66
pubmed: 7687008
Mol Gen Genet. 1995 Jan 20;246(2):174-9
pubmed: 7862088
Microbiol Rev. 1994 Sep;58(3):401-65
pubmed: 7968921
J Biol Chem. 2007 Jun 29;282(26):19247-58
pubmed: 17483090
Genetics. 2002 Mar;160(3):851-9
pubmed: 11901106
Nucleic Acids Res. 2011 Aug;39(14):6305-14
pubmed: 21504984
J Biol Chem. 2012 Oct 12;287(42):35621-35630
pubmed: 22902627
Nucleic Acids Res. 2022 Jan 25;50(2):937-951
pubmed: 34951472
Crit Rev Biochem Mol Biol. 2008 Sep-Oct;43(5):289-318
pubmed: 18937104
J Biol Chem. 2019 Jan 18;294(3):852-860
pubmed: 30482842
J Biol Chem. 1992 Oct 15;267(29):20648-58
pubmed: 1400384
PLoS Genet. 2018 Jan 19;14(1):e1007161
pubmed: 29351274
Environ Mol Mutagen. 2019 May;60(4):368-384
pubmed: 30447030
Mol Cell. 2001 Oct;8(4):734-6
pubmed: 11684009
Microbiol Mol Biol Rev. 1999 Dec;63(4):751-813, table of contents
pubmed: 10585965
J Bacteriol. 1996 Jan;178(1):184-90
pubmed: 8550414
Cell. 1997 Oct 31;91(3):347-56
pubmed: 9363943
Genes (Basel). 2022 Feb 27;13(3):
pubmed: 35327990
Methods Mol Biol. 2018;1672:107-118
pubmed: 29043620
Nucleic Acids Res. 2016 Sep 19;44(16):7691-9
pubmed: 27257075
J Biol Chem. 1999 Nov 5;274(45):31763-6
pubmed: 10542196
Mol Cell. 1999 Aug;4(2):281-6
pubmed: 10488344
Nucleic Acids Res. 2017 Jun 2;45(10):5877-5886
pubmed: 28369478