Heat stress during grain filling regulates seed germination through alterations of DNA methylation in barley (Hordeum vulgare L.).
Barley
DNA methylation
Grain filling
Heat stress
Seed germination
Journal
Plant molecular biology
ISSN: 1573-5028
Titre abrégé: Plant Mol Biol
Pays: Netherlands
ID NLM: 9106343
Informations de publication
Date de publication:
Nov 2022
Nov 2022
Historique:
received:
30
12
2021
accepted:
24
04
2022
pubmed:
18
5
2022
medline:
15
11
2022
entrez:
17
5
2022
Statut:
ppublish
Résumé
Alterations in DNA methylation levels of ROS, GA and ABA related gene promoters cause transcriptional changes upon imbibition to induce seed germination in barley seeds exposed to heat stress during grain filling. Environmental changes, especially changes in temperature, during seed development affect germination in several plant species. We have previously shown that heat stress during rice grain filling alters DNA methylation, an epigenetic mark important for gene silencing, regulates transcript levels of phytohormone metabolism genes, and delays seed germination. However, whether this phenomenon is present in other plant species remained to be elucidated. In this study, we compared seeds germination of barley (Hordeum vulgare L.) plants grown at 15 °C (control) or 25 °C (heat stress) during grain filling. Heat stress during grain filling significantly promoted seed germination in comparison with the control. The phytohormone gibberellic acid (GA) and reactive oxygen species produced by NADPH oxidases promote seed germination, whereas phytohormone abscisic acid (ABA) suppresses seed germination. We found that in heat-stressed seeds, genes related to ABA biosynthesis (HvNCED1 and 2) were significantly suppressed, whereas genes related to ABA catabolism (HvABA8'OH) and GA biosynthesis (HvHA20ox, HvGA3ox), and NADPH oxidase (HvRboh) genes were significantly upregulated after imbibition. Using MeDIP-qPCR, we showed that the promoters of HvNCED were hyper-methylated, and those of HvABA8'OH1, HvABA8'OH3, HvGA3ox2, and HvRbohF2 were hypo-methylated in heat treated seeds. Taken together, our data suggest that heat stress during grain filling affects DNA methylation of germination-related genes and promotes seed germination in barley.
Identifiants
pubmed: 35581415
doi: 10.1007/s11103-022-01278-5
pii: 10.1007/s11103-022-01278-5
doi:
Substances chimiques
Plant Growth Regulators
0
gibberellic acid
BU0A7MWB6L
Gibberellins
0
Abscisic Acid
72S9A8J5GW
Types de publication
Journal Article
Langues
eng
Sous-ensembles de citation
IM
Pagination
325-332Subventions
Organisme : Japan Society for the Promotion of Science
ID : 20H02971
Organisme : Japan Society for the Promotion of Science
ID : 21KK0108
Organisme : Japan Science and Technology Agency
ID : JPMJMI21E3
Informations de copyright
© 2022. The Author(s), under exclusive licence to Springer Nature B.V.
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