Multimodal Imaging Mass Spectrometry of Murine Gastrointestinal Tract with Retained Luminal Content.

Animals Gastrointestinal Tract / chemistry Ions Lipids / analysis Mice Multimodal Imaging Proteins / analysis Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization / methods
MALDI IMS gastrointestinal tract

Journal

Journal of the American Society for Mass Spectrometry
ISSN: 1879-1123
Titre abrégé: J Am Soc Mass Spectrom
Pays: United States
ID NLM: 9010412

Informations de publication

Date de publication:
01 Jun 2022
Historique:
pubmed: 12 5 2022
medline: 3 6 2022
entrez: 11 5 2022
Statut: ppublish

Résumé

The gastrointestinal tract, including luminal content, harbors a complex mixture of microorganisms, host dietary content, and immune factors. Existing imaging approaches remove luminal content and only visualize small regions of the GI tract. Here, we demonstrate a workflow for multimodal imaging using matrix-assisted laser desorption/ionization imaging mass spectrometry, autofluorescence, and bright field microscopy for mapping intestinal tissue and luminal content. Results comparing tissue and luminal content in control murine tissue show both unique molecular and elemental distributions and abundances using multimodal protein, lipid, and elemental imaging. For instance, lipid PC(42:1) is 2× higher intensity in luminal content than tissue, while PC(32:0) is 80× higher intensity in tissue. Additionally, some ions such as the protein at

Identifiants

DOI: 10.1021/jasms.1c00360 PMID: 35545232 PMC: PMC9264265
pubmed: 35545232
doi: 10.1021/jasms.1c00360
pmc: PMC9264265
mid: NIHMS1819205
doi:

Substances chimiques

Ions 0
Lipids 0
Proteins 0

Types de publication

Journal Article

Langues

eng

Sous-ensembles de citation

IM

Pagination

1073-1076

Subventions

Organisme : NIAID NIH HHS
ID : R01 AI138581
Pays : United States
Organisme : NIAID NIH HHS
ID : R01 AI145992
Pays : United States
Organisme : NIH HHS
ID : S10 OD012359
Pays : United States
Organisme : NIAID NIH HHS
ID : T32 AI112541
Pays : United States

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Auteurs

Emma R Guiberson (ER)

Mass Spectrometry Research Center, Vanderbilt University, Nashville, Tennessee 37203, United States.
Department of Chemistry, Vanderbilt University, Nashville, Tennessee 37203, United States.
ORCID: 0000-0002-1579-7820

Christopher J Good (CJ)

Mass Spectrometry Research Center, Vanderbilt University, Nashville, Tennessee 37203, United States.
Department of Chemistry, Vanderbilt University, Nashville, Tennessee 37203, United States.

Aaron G Wexler (AG)

Vanderbilt Institute for Infection, Immunology, and Inflammation, Vanderbilt University Medical Center, Nashville, Tennessee 37203, United States.
Department of Pathology, Microbiology, and Immunology, Vanderbilt University Medical Center, Nashville, Tennessee 37203, United States.
ORCID: 0000-0001-7926-089X

Eric P Skaar (EP)

Vanderbilt Institute for Infection, Immunology, and Inflammation, Vanderbilt University Medical Center, Nashville, Tennessee 37203, United States.
Department of Pathology, Microbiology, and Immunology, Vanderbilt University Medical Center, Nashville, Tennessee 37203, United States.

Jeffrey M Spraggins (JM)

Mass Spectrometry Research Center, Vanderbilt University, Nashville, Tennessee 37203, United States.
Department of Chemistry, Vanderbilt University, Nashville, Tennessee 37203, United States.
Department of Biochemistry, Vanderbilt University, Nashville, Tennessee 37203, United States.
Department of Cell and Developmental Biology, Vanderbilt University, Nashville, Tennessee 37203, United States.
ORCID: 0000-0001-9198-5498

Richard M Caprioli (RM)

Department of Chemistry, Vanderbilt University, Nashville, Tennessee 37203, United States.
Department of Biochemistry, Vanderbilt University, Nashville, Tennessee 37203, United States.
Department of Medicine, Vanderbilt University, Nashville, Tennessee 37203g, United States.
Department of Pharmacology, Vanderbilt University, Nashville, Tennessee 37203, United States.

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Classifications MeSH

Recherchemedicale.com › Eucaryotes › Animaux › Multimodal Imaging Mass Spectrometry of Murine...
Recherchemedicale.com › Système digestif › Tube digestif › Multimodal Imaging Mass Spectrometry of Murine...
Recherchemedicale.com › Produits chimiques inorganiques › Électrolytes › Ions › Multimodal Imaging Mass Spectrometry of Murine...
Recherchemedicale.com › Lipides › Multimodal Imaging Mass Spectrometry of Murine...
Recherchemedicale.com › Eucaryotes › Animaux › Chordés › Vertébrés › Mammifères › Eutheria › Rodentia › Muridae › Murinae › Souris › Multimodal Imaging Mass Spectrometry of Murine...
Recherchemedicale.com › Diagnostic › Techniques et procédures diagnostiques › Imagerie diagnostique › Imagerie multimodale › Multimodal Imaging Mass Spectrometry of Murine...
Recherchemedicale.com › Acides aminés, peptides et protéines › Protéines › Multimodal Imaging Mass Spectrometry of Murine...
Recherchemedicale.com › Techniques d'investigation › Techniques de chimie analytique › Spectrométrie de masse › Spectrométrie de masse MALDI › Multimodal Imaging Mass Spectrometry of Murine...