Stochastic biosensors based on N- and S-doped graphene for the enantioanalysis of aspartic acid in biological samples.


Journal

RSC advances
ISSN: 2046-2069
Titre abrégé: RSC Adv
Pays: England
ID NLM: 101581657

Informations de publication

Date de publication:
01 Jul 2021
Historique:
received: 15 03 2021
accepted: 22 06 2021
entrez: 28 4 2022
pubmed: 29 4 2022
medline: 29 4 2022
Statut: epublish

Résumé

Metabolomics plays a very important role in cancer mechanism and also in the early diagnosis of cancer. The enantioanalysis of aminoacids found in biological samples may favor the development of new screening tests for the early diagnosis of cancer. Two stochastic biosensors, based on the immobilization of α-hemolysin and hemin in exfoliated reduced graphene modified with nitrogen and sulphur, were proposed for the enantioanalysis of aspartic acid in biological samples (whole blood, urine, saliva, and tissue). The proposed biosensors showed low limits of quantification, high sensitivity, and large linear concentration ranges. The recoveries of d- and l-aspartic acid in biological samples were higher than 95.00%, with a relative standard deviation lower than 1.00%.

Identifiants

pubmed: 35479820
doi: 10.1039/d1ra02066h
pii: d1ra02066h
pmc: PMC9036598
doi:

Types de publication

Journal Article

Langues

eng

Pagination

23301-23309

Informations de copyright

This journal is © The Royal Society of Chemistry.

Déclaration de conflit d'intérêts

The authors declare no conflict of interest. The funders had no role in the design of the study; in the collection, analyses, or interpretation of data; in the writing of the manuscript, or in the decision to publish the results.

Références

Int J Clin Exp Pathol. 2018 May 01;11(5):2323-2336
pubmed: 31938344
Nat Nanotechnol. 2013 Apr;8(4):235-46
pubmed: 23552117
Nutrients. 2019 Sep 12;11(9):
pubmed: 31547425
Sensors (Basel). 2020 Apr 24;20(8):
pubmed: 32344622
J Chromatogr. 1986 Oct 10;368(1):135-43
pubmed: 3782361
Amino Acids. 2007 Feb;32(2):213-24
pubmed: 17031479
Int J Oncol. 2017 Jul;51(1):5-17
pubmed: 28535006
Analyst. 2007 Jul;132(7):615-32
pubmed: 17592579
PLoS One. 2012;7(7):e40459
pubmed: 22792336
J Sep Sci. 2008 Sep;31(16-17):3088-97
pubmed: 18785139
Brain Res Rev. 2007 Feb;53(2):215-34
pubmed: 17118457
Ageing Res Rev. 2002 Feb;1(1):43-59
pubmed: 12039448
J Chromatogr A. 2013 Mar 29;1283:9-19
pubmed: 23415444
J Chromatogr A. 2003 May 16;997(1-2):145-53
pubmed: 12830887
Nanomaterials (Basel). 2020 Aug 04;10(8):
pubmed: 32759829
Talanta. 2019 May 1;196:182-190
pubmed: 30683349
J Sep Sci. 2009 Sep;32(18):3184-90
pubmed: 19697316
Talanta. 2008 Oct 19;77(1):319-24
pubmed: 18804640

Auteurs

Raluca-Ioana Stefan-van Staden (RI)

Laboratory of Electrochemistry and PATLAB, National Institute of Research for Electrochemistry and Condensed Matter 202 Splaiul Independentei Str. 060021 Bucharest-6 Romania ralucavanstaden@gmail.com i.ruxandra04@yahoo.com.
Faculty of Applied Chemistry and Material Science, Politehnica University of Bucharest Bucharest Romania.

Damaris-Cristina Gheorghe (DC)

Laboratory of Electrochemistry and PATLAB, National Institute of Research for Electrochemistry and Condensed Matter 202 Splaiul Independentei Str. 060021 Bucharest-6 Romania ralucavanstaden@gmail.com i.ruxandra04@yahoo.com.
Faculty of Applied Chemistry and Material Science, Politehnica University of Bucharest Bucharest Romania.

Ruxandra-Maria Ilie-Mihai (RM)

Laboratory of Electrochemistry and PATLAB, National Institute of Research for Electrochemistry and Condensed Matter 202 Splaiul Independentei Str. 060021 Bucharest-6 Romania ralucavanstaden@gmail.com i.ruxandra04@yahoo.com.

Lucian-Barbu Tudoran (LB)

National Institute for Research and Development of Isotopic and Molecular Technologies Donat Street 67-103 Cluj-Napoca Romania.

Stela Maria Pruneanu (SM)

National Institute for Research and Development of Isotopic and Molecular Technologies Donat Street 67-103 Cluj-Napoca Romania.

Classifications MeSH