Cell-type-specific gene expression profile by laser capture microdissection on mirror sections.
Celiac disease
Cell-specific gene expression profiles
Laser capture microdissection
Mirror sections
γδ + IELs
Journal
Journal of immunological methods
ISSN: 1872-7905
Titre abrégé: J Immunol Methods
Pays: Netherlands
ID NLM: 1305440
Informations de publication
Date de publication:
06 2022
06 2022
Historique:
received:
21
12
2021
revised:
29
03
2022
accepted:
20
04
2022
pubmed:
28
4
2022
medline:
24
5
2022
entrez:
27
4
2022
Statut:
ppublish
Résumé
Immuno-laser capture microdissection (Immuno-LCM) has been used to analyze cell-specific gene expression profiles. However, the usefulness of such a technique is frequently limited by RNA degradation. We, therefore, developed a rapid protocol of LCM on mirror sections, which allows for preserving RNA integrity. With such a procedure, we investigated cell-type-specific gene expression of γδ intraepithelial lymphocytes (IELs) in untreated celiac disease (CD). An increase in TGF-β mRNA expression levels was observed in γδ + IELs compared to intestinal enterocytes (IEs), whereas anti-inflammatory IL-10 mRNA production from γδ + IELs was lower compared to IEs. In untreated CD patients, the production of anti-inflammatory cytokines by γδ + IELs is suggestive of a regulatory function, thus playing a critical role in limiting inflammation. This work underscores the importance of LCM on mirror sections as a valuable tool to perform cell-type-specific molecular analysis in tissue.
Identifiants
pubmed: 35476945
pii: S0022-1759(22)00063-1
doi: 10.1016/j.jim.2022.113276
pii:
doi:
Substances chimiques
Cytokines
0
RNA, Messenger
0
Types de publication
Journal Article
Research Support, Non-U.S. Gov't
Langues
eng
Sous-ensembles de citation
IM
Pagination
113276Informations de copyright
Copyright © 2022 Elsevier B.V. All rights reserved.