The Impact of Prolonged Inflammation on Wound Healing.
inflammation
prolonged inflammation
resiquimod R848
wound healing
wound model
Journal
Biomedicines
ISSN: 2227-9059
Titre abrégé: Biomedicines
Pays: Switzerland
ID NLM: 101691304
Informations de publication
Date de publication:
06 Apr 2022
06 Apr 2022
Historique:
received:
02
03
2022
revised:
10
03
2022
accepted:
29
03
2022
entrez:
23
4
2022
pubmed:
24
4
2022
medline:
24
4
2022
Statut:
epublish
Résumé
The treatment of chronic wounds still challenges modern medicine because of these wounds' heterogenic pathophysiology. Processes such as inflammation, ischemia and bacterial infection play major roles in the progression of a chronic wound. In recent years, preclinical wound models have been used to understand the underlying processes of chronic wound formation. However, the wound models used to investigate chronic wounds often lack translatability from preclinical models to patients, and often do not take exaggerated inflammation into consideration. Therefore, we aimed to investigate prolonged inflammation in a porcine wound model by using resiquimod, a TLR7 and TLR8 agonist. Pigs received full thickness excisional wounds, where resiquimod was applied daily for 6 days, and untreated wounds served as controls. Dressing change, visual documentation and wound scoring were performed daily. Biopsies were collected for histological as well as gene expression analysis. Resiquimod application on full thickness wounds induced a visible inflammation of wounds, resulting in delayed wound healing compared to non-treated control wounds. Gene expression analysis revealed high levels of IL6, MMP1 and CD68 expression after resiquimod application, and histological analysis showed increased immune cell infiltration. By using resiquimod, we were able to show that prolonged inflammation delayed wound healing, which is often observed in chronic wounds in patients. The model we used shows the importance of inflammation in wound healing and gives an insight into the progression of chronic wounds.
Identifiants
pubmed: 35453606
pii: biomedicines10040856
doi: 10.3390/biomedicines10040856
pmc: PMC9025535
pii:
doi:
Types de publication
Journal Article
Langues
eng
Subventions
Organisme : Austrian Federal Ministry of Climate Action, Environment, Energy, Mobility, Innovation and Technology
ID : BMVIT-612.018/0016-lll/I1/2018-Tec.Reg
Références
Antimicrob Agents Chemother. 2008 Feb;52(2):477-82
pubmed: 18039918
Biomed Tech (Berl). 2018 Oct 25;63(5):547-556
pubmed: 30028724
Wound Repair Regen. 2014 Jan-Feb;22(1):34-42
pubmed: 24134795
Int Wound J. 2016 Oct;13(5):927-35
pubmed: 25586463
Br J Dermatol. 2019 Feb;180(2):297-305
pubmed: 30171698
Wound Repair Regen. 2009 May-Jun;17(3):354-9
pubmed: 19660043
Clin Plast Surg. 2005 Apr;32(2):195-208
pubmed: 15814117
Nat Rev Immunol. 2004 Jul;4(7):499-511
pubmed: 15229469
Wound Repair Regen. 2019 Jan;27(1):114-125
pubmed: 30362646
J Clin Med. 2020 Dec 24;10(1):
pubmed: 33374372
J Antimicrob Chemother. 2001 Dec;48(6):751-5
pubmed: 11733457
Wound Repair Regen. 2021 Sep;29(5):725-731
pubmed: 33768649
J Int Med Res. 2009 Sep-Oct;37(5):1528-42
pubmed: 19930861
Antimicrob Agents Chemother. 2003 Dec;47(12):3846-52
pubmed: 14638493
Skin Res Technol. 2013 Nov;19(4):474-83
pubmed: 23581539
Blood. 2015 Sep 17;126(12):1452-61
pubmed: 26228486
Int Wound J. 2017 Dec;14(6):1225-1236
pubmed: 28730726
Proteomics. 2013 Sep;13(17):2670-81
pubmed: 23798543
Cureus. 2021 Sep 4;13(9):e17720
pubmed: 34650894
Int J Mol Sci. 2022 Feb 05;23(3):
pubmed: 35163741
J Lipid Res. 2019 Apr;60(4):758-766
pubmed: 30696699
Lab Invest. 2016 Apr;96(4):439-49
pubmed: 26855364
J Mol Med (Berl). 2009 Feb;87(2):125-38
pubmed: 19089397
J Environ Pathol Toxicol Oncol. 2002;21(1):71-8
pubmed: 11934016
Adv Wound Care (New Rochelle). 2017 Oct 1;6(10):344-355
pubmed: 29062591
Adv Wound Care (New Rochelle). 2015 Jul 1;4(7):382-388
pubmed: 26155380
Pharm Res. 2012 Jul;29(7):1808-20
pubmed: 22419258
J Dermatol Sci. 2018 Apr;90(1):3-12
pubmed: 29289417
Int J Mol Sci. 2017 Jul 03;18(7):
pubmed: 28671607
Adv Skin Wound Care. 2000 May-Jun;13(2 Suppl):6-11
pubmed: 11074996
Am J Pathol. 2008 Jul;173(1):265-77
pubmed: 18535184
Cell Death Dis. 2018 May 2;9(5):499
pubmed: 29717111
Nucleic Acids Res. 2001 May 1;29(9):e45
pubmed: 11328886
Arch Dermatol. 2004 Dec;140(12):1490-5
pubmed: 15611427
J Vis Exp. 2013 May 28;(75):e50265
pubmed: 23748713
Biomed Pharmacother. 2020 Jan;121:109613
pubmed: 31707336
Adv Drug Deliv Rev. 2019 Jun;146:97-125
pubmed: 30267742
Wound Repair Regen. 2011 May-Jun;19(3):309-15
pubmed: 21539648
J Invest Dermatol. 2012 Aug;132(8):1955-8
pubmed: 22797299
Wound Repair Regen. 2016 Jan-Feb;24(1):26-34
pubmed: 26562746
J Immunol. 2009 May 1;182(9):5836-45
pubmed: 19380832
Adv Wound Care (New Rochelle). 2017 Oct 1;6(10):330-343
pubmed: 29062590