Nutrient and salt depletion synergistically boosts glucose metabolism in individual Escherichia coli cells.
Journal
Communications biology
ISSN: 2399-3642
Titre abrégé: Commun Biol
Pays: England
ID NLM: 101719179
Informations de publication
Date de publication:
20 04 2022
20 04 2022
Historique:
received:
11
02
2022
accepted:
30
03
2022
entrez:
21
4
2022
pubmed:
22
4
2022
medline:
23
4
2022
Statut:
epublish
Résumé
The interaction between a cell and its environment shapes fundamental intracellular processes such as cellular metabolism. In most cases growth rate is treated as a proximal metric for understanding the cellular metabolic status. However, changes in growth rate might not reflect metabolic variations in individuals responding to environmental fluctuations. Here we use single-cell microfluidics-microscopy combined with transcriptomics, proteomics and mathematical modelling to quantify the accumulation of glucose within Escherichia coli cells. In contrast to the current consensus, we reveal that environmental conditions which are comparatively unfavourable for growth, where both nutrients and salinity are depleted, increase glucose accumulation rates in individual bacteria and population subsets. We find that these changes in metabolic function are underpinned by variations at the translational and posttranslational level but not at the transcriptional level and are not dictated by changes in cell size. The metabolic response-characteristics identified greatly advance our fundamental understanding of the interactions between bacteria and their environment and have important ramifications when investigating cellular processes where salinity plays an important role.
Identifiants
pubmed: 35444215
doi: 10.1038/s42003-022-03336-6
pii: 10.1038/s42003-022-03336-6
pmc: PMC9021252
doi:
Substances chimiques
Escherichia coli Proteins
0
Glucose
IY9XDZ35W2
Types de publication
Journal Article
Research Support, Non-U.S. Gov't
Langues
eng
Sous-ensembles de citation
IM
Pagination
385Subventions
Organisme : Biotechnology and Biological Sciences Research Council
ID : BB/K003240/1
Pays : United Kingdom
Organisme : Biotechnology and Biological Sciences Research Council
ID : BB/V008021/1
Pays : United Kingdom
Organisme : Wellcome Trust
ID : WT101650MA
Pays : United Kingdom
Organisme : Wellcome Trust
Pays : United Kingdom
Organisme : Wellcome Trust
ID : 204909/Z/16/Z
Pays : United Kingdom
Organisme : Medical Research Council
ID : MCPC17189
Pays : United Kingdom
Organisme : Wellcome Trust
ID : WT097835MF
Pays : United Kingdom
Informations de copyright
© 2022. The Author(s).
Références
J Biol Chem. 2005 Jul 22;280(29):26880-5
pubmed: 15917245
J Appl Microbiol. 2017 Sep;123(3):570-581
pubmed: 28383815
Mol Microbiol. 1996 Jun;20(5):911-7
pubmed: 8809744
Appl Environ Microbiol. 2014 Aug;80(15):4745-56
pubmed: 24858086
Anal Biochem. 1999 May 15;270(1):88-96
pubmed: 10328769
Proc Natl Acad Sci U S A. 2003 Feb 4;100(3):825-32
pubmed: 12556564
Cell. 2016 Jul 28;166(3):729-739
pubmed: 27471967
Curr Biol. 2015 Feb 2;25(3):385-391
pubmed: 25544609
Best Pract Res Clin Gastroenterol. 2016 Apr;30(2):145-59
pubmed: 27086882
Curr Biol. 2010 Jun 22;20(12):1099-103
pubmed: 20537537
J Proteome Res. 2013 Jun 7;12(6):2611-21
pubmed: 23590516
Sci Rep. 2016 Apr 06;6:24055
pubmed: 27048851
Environ Microbiol. 2015 Jul;17(7):2542-56
pubmed: 25655651
EcoSal Plus. 2008 Sep;3(1):
pubmed: 26443723
Nat Microbiol. 2018 Aug;3(8):939-947
pubmed: 30038306
Environ Microbiol. 2003 Mar;5(3):202-11
pubmed: 12588299
Phys Rev Lett. 2016 Jul 15;117(3):038001
pubmed: 27472142
Annu Rev Microbiol. 2011;65:189-213
pubmed: 21639793
J Bacteriol. 2011 May;193(9):2252-60
pubmed: 21398557
Biosci Biotechnol Biochem. 1996 Nov;60(11):1899-901
pubmed: 8987871
FEMS Microbiol Ecol. 2007 Dec;62(3):345-53
pubmed: 17916075
mBio. 2021 Aug 31;12(4):e0090921
pubmed: 34281389
J Bacteriol. 1988 Jul;170(7):3283-6
pubmed: 3384812
BMC Biol. 2017 Dec 21;15(1):121
pubmed: 29262826
PLoS Genet. 2017 Dec 18;13(12):e1007122
pubmed: 29253903
Nucleic Acids Res. 2015 Aug 18;43(14):e92
pubmed: 25883145
OMICS. 2012 May;16(5):284-7
pubmed: 22455463
Adv Protein Chem Struct Biol. 2017;109:265-304
pubmed: 28683921
Genome Biol. 2014;15(12):550
pubmed: 25516281
NPJ Syst Biol Appl. 2017 Jan 05;3:16035
pubmed: 28725483
J Microbiol Methods. 2000 Sep;42(1):87-96
pubmed: 11000435
R J. 2016 Aug;8(1):289-317
pubmed: 27818791
Front Microbiol. 2015 Apr 16;6:243
pubmed: 25932020
ISME J. 2018 May;12(5):1199-1209
pubmed: 29335635
Science. 2010 Jul 30;329(5991):533-8
pubmed: 20671182
Metab Eng. 1999 Oct;1(4):320-33
pubmed: 10937825
Appl Environ Microbiol. 2006 Nov;72(11):7022-8
pubmed: 16950912
Sci Rep. 2019 Jul 12;9(1):10123
pubmed: 31300741
Elife. 2022 Jun 07;11:
pubmed: 35670099
BMC Genomics. 2017 Apr 17;18(1):301
pubmed: 28412930
ACS Nano. 2020 Feb 25;14(2):1609-1622
pubmed: 31794180
J Bacteriol. 1994 Sep;176(18):5847-51
pubmed: 8083177
Ann Appl Stat. 2016 Jun;10(2):946-963
pubmed: 28367255
ACS Infect Dis. 2021 Jun 11;7(6):1848-1858
pubmed: 34000805
Nat Rev Microbiol. 2019 Nov;17(11):651-664
pubmed: 31485032
BMC Microbiol. 2013 Nov 15;13:258
pubmed: 24238347
Philos Trans R Soc Lond B Biol Sci. 2019 Nov 25;374(1786):20190076
pubmed: 31587644
Microbiology (Reading). 1995 Jan;141 ( Pt 1):71-8
pubmed: 7894722
EuPA Open Proteom. 2015 Jun;7:11-19
pubmed: 25821719
Front Microbiol. 2020 Nov 17;11:547458
pubmed: 33281753
Sci Rep. 2016 Apr 25;6:24834
pubmed: 27109914
Medicine (Baltimore). 2017 Nov;96(47):e8423
pubmed: 29381919
Lab Chip. 2020 Aug 7;20(15):2765-2775
pubmed: 32613221
Microbiol Rev. 1987 Sep;51(3):365-79
pubmed: 3312987
J Bacteriol. 1995 Jun;177(11):3087-94
pubmed: 7768805
Microbiol Mol Biol Rev. 2003 Dec;67(4):593-656
pubmed: 14665678
Environ Pollut. 2013 Feb;173:157-67
pubmed: 23202646
Rev Sci Instrum. 2014 Feb;85(2):023708
pubmed: 24593372
Nat Rev Microbiol. 2015 Aug;13(8):497-508
pubmed: 26145732
Appl Environ Microbiol. 2013 Jun;79(12):3703-15
pubmed: 23563955
Biochem J. 1979 Jan 15;178(1):97-101
pubmed: 373752
Appl Microbiol Biotechnol. 1996 Nov;46(4):400-4
pubmed: 8987729
Biochem J. 1976 May 15;156(2):477-80
pubmed: 782451
Science. 2018 Jan 19;359(6373):320-325
pubmed: 29348236
Sci Rep. 2017 Apr 18;7:45303
pubmed: 28417974
Biophys J. 2015 Aug 4;109(3):521-8
pubmed: 26244734
FEMS Microbiol Rev. 2008 Nov;32(6):891-907
pubmed: 18647176
Mol Biosyst. 2011 Nov;7(11):3087-93
pubmed: 21850335
Nat Rev Microbiol. 2014 Apr;12(4):263-73
pubmed: 24590245
Front Microbiol. 2019 Jan 10;9:3272
pubmed: 30687263
PLoS Biol. 2021 Oct 12;19(10):e3001406
pubmed: 34637438
J Biol Chem. 1999 Mar 5;274(10):6091-6
pubmed: 10037691
Philos Trans R Soc Lond B Biol Sci. 2018 Dec 3;374(1764):
pubmed: 30509913
Biochim Biophys Acta. 1996 Feb 9;1289(1):5-9
pubmed: 8605231
Nat Rev Microbiol. 2010 Jun;8(6):401-12
pubmed: 20453875
J Bacteriol. 2006 Oct;188(20):7165-75
pubmed: 17015655
RSC Chem Biol. 2020 Nov 17;1(5):395-404
pubmed: 34458770
Science. 2010 Nov 19;330(6007):1099-102
pubmed: 21097934
Mol Syst Biol. 2013 Oct 01;9:693
pubmed: 24084808
Proc Natl Acad Sci U S A. 2002 Jul 23;99(15):9789-94
pubmed: 12119404
Philos Trans R Soc Lond B Biol Sci. 2019 Nov 25;374(1786):20180442
pubmed: 31587633
Front Microbiol. 2018 Aug 15;9:1739
pubmed: 30158905
Curr Opin Microbiol. 2018 Oct;45:30-38
pubmed: 29477028