The Effects of the Combination of Mesenchymal Stromal Cells and Nanofiber-Hydrogel Composite on Repair of the Contused Spinal Cord.
astrocytes
axon growth
inflammation
macrophages
nanofiber-hydrogel composite
secondary injury
spinal cord injury
Journal
Cells
ISSN: 2073-4409
Titre abrégé: Cells
Pays: Switzerland
ID NLM: 101600052
Informations de publication
Date de publication:
28 03 2022
28 03 2022
Historique:
received:
17
02
2022
revised:
17
03
2022
accepted:
23
03
2022
entrez:
12
4
2022
pubmed:
13
4
2022
medline:
14
4
2022
Statut:
epublish
Résumé
A bone marrow-derived mesenchymal stromal cell (MSC) transplant and a bioengineered nanofiber-hydrogel composite (NHC) have been shown to stimulate nervous tissue repair in the contused spinal cord in rodent models. Here, these two modalities were combined to assess their repair effects in the contused spinal cord in adult rats. Cohorts of contused rats were treated with MSC in NHC (MSC-NHC), MSC in phosphate-buffered saline (MSC-PBS), NHC, or PBS injected into the contusion site at 3 days post-injury. One week after injury, there were significantly fewer CD68+ cells in the contusion with MSC-NHC and NHC, but not MSC-PBS. The reduction in CD86+ cells in the injury site with MSC-NHC was mainly attributed to NHC. One and eight weeks after injury, we found a greater CD206+/CD86+ cell ratio with MSC-NHC or NHC, but not MSC-PBS, indicating a shift from a pro-inflammatory towards an anti-inflammatory milieu in the injury site. Eight weeks after injury, the injury size was significantly reduced with MSC-NHC, NHC, and MSC-PBS. At this time, astrocyte, and axon presence in the injury site was greater with MSC-NHC compared with MSC-PBS. We did not find a significant effect of NHC on MSC transplant survival, and hind limb function was similar across all groups. However, we did find fewer macrophages at 1 week post-injury, more macrophages polarized towards a pro-regenerative phenotype at 1 and 8 weeks after injury, and reduced injury volume, more astrocytes, and more axons at 8 weeks after injury in rats with MSC-NHC and NHC alone compared with MSC-PBS; these findings were especially significant between rats with MSC-NHC and MSC-PBS. The data support further study in the use of an NHC-MSC combination transplant in the contused spinal cord.
Identifiants
pubmed: 35406701
pii: cells11071137
doi: 10.3390/cells11071137
pmc: PMC8997442
pii:
doi:
Substances chimiques
Hydrogels
0
Types de publication
Journal Article
Research Support, U.S. Gov't, Non-P.H.S.
Research Support, N.I.H., Extramural
Research Support, Non-U.S. Gov't
Langues
eng
Sous-ensembles de citation
IM
Subventions
Organisme : NINDS NIH HHS
ID : R21 NS085714
Pays : United States
Références
J Neurosci. 2009 Oct 28;29(43):13435-44
pubmed: 19864556
Int J Mol Sci. 2018 May 17;19(5):
pubmed: 29772841
J Neuropathol Exp Neurol. 2002 Jul;61(7):623-33
pubmed: 12125741
Neurospine. 2021 Jun;18(2):261-270
pubmed: 33494555
Proc Natl Acad Sci U S A. 2013 Oct 22;110(43):17253-8
pubmed: 24101477
J Biomol Tech. 2016 Sep;27(3):90-7
pubmed: 27182204
J Neurotrauma. 2003 Feb;20(2):179-93
pubmed: 12675971
Cytotherapy. 2013 Apr;15(4):434-48
pubmed: 23376106
J Neurotrauma. 2012 May 20;29(8):1614-25
pubmed: 22233298
J Neurotrauma. 1995 Feb;12(1):1-21
pubmed: 7783230
Cell Mol Neurobiol. 2006 Oct-Nov;26(7-8):1167-80
pubmed: 16897366
Neuron. 2014 Sep 3;83(5):1098-116
pubmed: 25132469
Exp Eye Res. 2019 Aug;185:107671
pubmed: 31108056
PLoS One. 2012;7(6):e39500
pubmed: 22745769
Proc Natl Acad Sci U S A. 1998 Mar 31;95(7):3908-13
pubmed: 9520466
J Neurosurg Spine. 2017 Mar;26(3):388-395
pubmed: 27885959
PLoS One. 2018 Jan 11;13(1):e0191040
pubmed: 29324807
Transplantation. 2010 Dec 27;90(12):1312-20
pubmed: 21042238
Pharmacol Ther. 2021 Nov 20;:108043
pubmed: 34813862
Cell Transplant. 2012;21(7):1561-75
pubmed: 22526408
Biomaterials. 2013 Jun;34(18):4439-51
pubmed: 23515178
Cell Transplant. 2006;15(7):563-77
pubmed: 17176609
Int J Mol Sci. 2019 May 31;20(11):
pubmed: 31159345
J Neurosurg. 2001 Oct;95(2 Suppl):221-4
pubmed: 11599840
Biomaterials. 2014 Feb;35(6):1924-31
pubmed: 24331711
Neuroscience. 2002;111(2):325-36
pubmed: 11983318
Sci Transl Med. 2019 May 1;11(490):
pubmed: 31043572
Cell Transplant. 2015;24(11):2209-20
pubmed: 25581479
J Surg Res. 2006 Dec;136(2):336-41
pubmed: 17045612
Mol Cell Proteomics. 2016 Feb;15(2):394-408
pubmed: 26695766
Stem Cells. 2008 Jan;26(1):99-107
pubmed: 17916800
Neurosci Lett. 2010 Oct 8;483(1):57-61
pubmed: 20678542
Neurosurg Clin N Am. 2021 Jul;32(3):305-313
pubmed: 34053718
Acta Biomater. 2015 Jan;11:204-11
pubmed: 25266504
Neurotherapeutics. 2018 Jul;15(3):578-587
pubmed: 29728851
Neurochem Int. 2018 May;115:80-84
pubmed: 29458076
Cell Transplant. 2018 Jul;27(7):1126-1139
pubmed: 29947256
Biomaterials. 2020 Jul;245:119978
pubmed: 32217415
Neuroscience. 2016 Oct 29;335:221-31
pubmed: 27586052
Exp Neurol. 1993 Feb;119(2):153-64
pubmed: 8432357
PLoS One. 2016 Mar 01;11(3):e0149876
pubmed: 26930594
J Neurotrauma. 2006 Mar-Apr;23(3-4):264-80
pubmed: 16629615
Mol Neurobiol. 2013 Jun;47(3):1011-9
pubmed: 23321790
J Neurotrauma. 2009 Dec;26(12):2313-22
pubmed: 19645530
Brain Res. 2015 Sep 4;1619:1-11
pubmed: 25578260