LC-MS/MS Method for Measurement of Thiopurine Nucleotides (TN) in Erythrocytes and Association of TN Concentrations With TPMT Enzyme Activity.
6-methylmercaptopurine
6-thioguanine
clinical evaluation
mass spectromerty
thiopurine methyl transferase
Journal
Frontiers in pharmacology
ISSN: 1663-9812
Titre abrégé: Front Pharmacol
Pays: Switzerland
ID NLM: 101548923
Informations de publication
Date de publication:
2022
2022
Historique:
received:
15
12
2021
accepted:
14
02
2022
entrez:
7
4
2022
pubmed:
8
4
2022
medline:
8
4
2022
Statut:
epublish
Résumé
Monitoring concentrations of thiopurine metabolites is used clinically to prevent adverse effects in patients on thiopurine drug therapy. We developed a LC-MS/MS method for the quantification of 6-thioguanine (6-TG) and 6-methylmercaptopurine (6-MMP) in red blood cells (RBCs). This method utilizes an automated cell washer for RBC separation from whole blood samples and washing of the separated RBCs. The lower limit of quantification of the method was 0.2 μmol/L for 6-TG (∼50 pmol/8 × 10
Identifiants
pubmed: 35387339
doi: 10.3389/fphar.2022.836812
pii: 836812
pmc: PMC8978547
doi:
Types de publication
Journal Article
Langues
eng
Pagination
836812Informations de copyright
Copyright © 2022 Bajaj, Kushnir, Kish-Trier, Law, Zuromski, Molinelli, McMillin and Johnson-Davis.
Déclaration de conflit d'intérêts
The authors declare that the research was conducted in the absence of any commercial or financial relationships that could be construed as a potential conflict of interest.
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