The Serological Sciences Network (SeroNet) for COVID-19: Depth and Breadth of Serology Assays and Plans for Assay Harmonization.

Journal

medRxiv : the preprint server for health sciences
Titre abrégé: medRxiv
Pays: United States
ID NLM: 101767986

Informations de publication

Date de publication:
14 Mar 2022
Historique:
pubmed: 10 3 2022
medline: 10 3 2022
entrez: 9 3 2022
Statut: epublish

Résumé

In October 2020, the National Cancer Institute (NCI) Serological Sciences Network (SeroNet) was established to study the immune response to COVID-19, and "to develop, validate, improve, and implement serological testing and associated technologies." SeroNet is comprised of 25 participating research institutions partnering with the Frederick National Laboratory for Cancer Research (FNLCR) and the SeroNet Coordinating Center. Since its inception, SeroNet has supported collaborative development and sharing of COVID-19 serological assay procedures and has set forth plans for assay harmonization. To facilitate collaboration and procedure sharing, a detailed survey was sent to collate comprehensive assay details and performance metrics on COVID-19 serological assays within SeroNet. In addition, FNLCR established a protocol to calibrate SeroNet serological assays to reference standards, such as the U.S. SARS-CoV-2 serology standard reference material and First WHO International Standard (IS) for anti-SARS-CoV-2 immunoglobulin (20/136), to facilitate harmonization of assay reporting units and cross-comparison of study data. SeroNet institutions reported development of a total of 27 ELISA methods, 13 multiplex assays, 9 neutralization assays, and use of 12 different commercial serological methods. FNLCR developed a standardized protocol for SeroNet institutions to calibrate these diverse serological assays to reference standards. SeroNet institutions have established a diverse array of COVID-19 serological assays to study the immune response to SARS-CoV-2 virus and vaccines. Calibration of SeroNet serological assays to harmonize results reporting will facilitate future pooled data analyses and study cross-comparisons.

Sections du résumé

Background UNASSIGNED
In October 2020, the National Cancer Institute (NCI) Serological Sciences Network (SeroNet) was established to study the immune response to COVID-19, and "to develop, validate, improve, and implement serological testing and associated technologies." SeroNet is comprised of 25 participating research institutions partnering with the Frederick National Laboratory for Cancer Research (FNLCR) and the SeroNet Coordinating Center. Since its inception, SeroNet has supported collaborative development and sharing of COVID-19 serological assay procedures and has set forth plans for assay harmonization.
Methods UNASSIGNED
To facilitate collaboration and procedure sharing, a detailed survey was sent to collate comprehensive assay details and performance metrics on COVID-19 serological assays within SeroNet. In addition, FNLCR established a protocol to calibrate SeroNet serological assays to reference standards, such as the U.S. SARS-CoV-2 serology standard reference material and First WHO International Standard (IS) for anti-SARS-CoV-2 immunoglobulin (20/136), to facilitate harmonization of assay reporting units and cross-comparison of study data.
Results UNASSIGNED
SeroNet institutions reported development of a total of 27 ELISA methods, 13 multiplex assays, 9 neutralization assays, and use of 12 different commercial serological methods. FNLCR developed a standardized protocol for SeroNet institutions to calibrate these diverse serological assays to reference standards.
Conclusions UNASSIGNED
SeroNet institutions have established a diverse array of COVID-19 serological assays to study the immune response to SARS-CoV-2 virus and vaccines. Calibration of SeroNet serological assays to harmonize results reporting will facilitate future pooled data analyses and study cross-comparisons.

Identifiants

DOI: 10.1101/2022.02.27.22271399 PMID: 35262095 PMC: PMC8902887
pubmed: 35262095
doi: 10.1101/2022.02.27.22271399
pmc: PMC8902887
pii:
doi:

Types de publication

Preprint

Langues

eng

Subventions

Organisme : NCI NIH HHS
ID : U54 CA260591
Pays : United States
Organisme : NCI NIH HHS
ID : U54 CA260492
Pays : United States
Organisme : NCI NIH HHS
ID : U54 CA260582
Pays : United States
Organisme : NCI NIH HHS
ID : U01 CA261276
Pays : United States
Organisme : NCI NIH HHS
ID : U01 CA260541
Pays : United States
Organisme : NCI NIH HHS
ID : U01 CA260469
Pays : United States
Organisme : NCI NIH HHS
ID : U01 CA260462
Pays : United States
Organisme : NCI NIH HHS
ID : U54 CA260543
Pays : United States
Organisme : NCI NIH HHS
ID : U54 CA260563
Pays : United States
Organisme : NIAID NIH HHS
ID : P01 AI078907
Pays : United States
Organisme : NCI NIH HHS
ID : U01 CA260526
Pays : United States
Organisme : NCI NIH HHS
ID : U01 CA260508
Pays : United States
Organisme : NCI NIH HHS
ID : U01 CA260539
Pays : United States
Organisme : NCI NIH HHS
ID : 75N91019D00024
Pays : United States

Commentaires et corrections

Type : UpdateIn

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Auteurs

Amy B Karger (AB)

Department of Laboratory Medicine and Pathology, University of Minnesota, Minneapolis, Minnesota.

James D Brien (JD)

Department of Molecular Microbiology & Immunology, Saint Louis University, Saint Louis, Missouri.

Jayne M Christen (JM)

Frederick National Laboratory for Cancer Research, Frederick, Maryland.

Santosh Dhakal (S)

W. Harry Feinstone Department of Molecular Microbiology and Immunology, The Johns Hopkins Bloomberg School of Public Health, Baltimore, Maryland.

Troy J Kemp (TJ)

Frederick National Laboratory for Cancer Research, Frederick, Maryland.

Sabra L Klein (SL)

W. Harry Feinstone Department of Molecular Microbiology and Immunology, The Johns Hopkins Bloomberg School of Public Health, Baltimore, Maryland.

Ligia A Pinto (LA)

Frederick National Laboratory for Cancer Research, Frederick, Maryland.

Lakshmanane Premkumar (L)

Department of Microbiology and Immunology, University of North Carolina, Chapel Hill, NC.

John D Roback (JD)

Department of Pathology and Laboratory Medicine, Emory University School of Medicine, Atlanta, Georgia.

Raquel A Binder (RA)

Department of Medicine, University of Massachusetts Chan Medical School, Worcester, Massachusetts.

Karl W Boehme (KW)

Department of Microbiology & Immunology, University of Arkansas for Medical Sciences, Little Rock, Arkansas.

Suresh Boppana (S)

Department of Pediatrics, University of Alabama at Birmingham, Birmingham, Alabama.
Department of Microbiology, University of Alabama at Birmingham, Birmingham, Alabama.

Carlos Cordon-Cardo (C)

Department of Pathology, Icahn School of Medicine at Mount Sinai, New York, New York.

James M Crawford (JM)

Department of Pathology and Laboratory Medicine, Donald and Barbara Zucker School of Medicine at Hofstra/Northwell, Hempstead, New York.

John L Daiss (JL)

MicroB-plex, Inc., Atlanta, Georgia.

Alan P Dupuis (AP)

Wadsworth Center, New York State Department of Health, Albany, New York.

Ana M Espino (AM)

Department of Microbiology and Medical Zoology, University of Puerto Rico-Medical Sciences Campus, San Juan, Puerto Rico.

Adolfo Firpo-Betancourt (A)

Department of Pathology, Icahn School of Medicine at Mount Sinai, New York, New York.

Catherine Forconi (C)

Department of Medicine, University of Massachusetts Chan Medical School, Worcester, Massachusetts.

J Craig Forrest (JC)

Department of Microbiology & Immunology, University of Arkansas for Medical Sciences, Little Rock, Arkansas.

Roxie C Girardin (RC)

Wadsworth Center, New York State Department of Health, Albany, New York.

Douglas A Granger (DA)

Salimetrics, LLC, Carlsbad, California.

Steve W Granger (SW)

Salimetrics, LLC, Carlsbad, California.

Natalie S Haddad (NS)

Division of Pulmonary, Allergy, Critical Care and Sleep Medicine, Department of Medicine, Emory University, Atlanta, Georgia.

Christopher D Heaney (CD)

Department of Environmental Health and Engineering, Johns Hopkins Bloomberg School of Public Health, Baltimore, Maryland.

Danielle T Hunt (DT)

Wadsworth Center, New York State Department of Health, Albany, New York.

Joshua L Kennedy (JL)

Departments of Pediatrics and Internal Medicine, University of Arkansas for Medical Sciences, Little Rock, Arkansas.
Arkansas Children's Research Institute, Little Rock, Arkansas.

Christopher L King (CL)

Department of Pathology, Case Western Reserve School of Medicine, Cleveland, Ohio.

Florian Krammer (F)

Department of Microbiology, Icahn School of Medicine at Mount Sinai, New York, New York.

Kate Kruczynski (K)

Department of Environmental Health and Engineering, Johns Hopkins Bloomberg School of Public Health, Baltimore, Maryland.

Joshua LaBaer (J)

Virginia G Piper Center for Personalized Diagnostics, Arizona State University Biodesign Institute, Tempe, Arizona.

F Eun-Hyung Lee (FE)

Division of Pulmonary, Allergy, Critical Care and Sleep Medicine, Department of Medicine, Emory University, Atlanta, Georgia.

William T Lee (WT)

Wadsworth Center, New York State Department of Health, Albany, New York.
Department of Biomedical Sciences, School of Public Health, University at Albany, Albany, New York.

Shan-Lu Liu (SL)

Center for Retrovirus Research, Department of Veterinary Biosciences, Department of Microbial Infection and Immunity, Viruses and Emerging Pathogens Program, Infectious Disease Institute, The Ohio State University, Columbus, Ohio.

Gerard Lozanski (G)

Department of Pathology, The Ohio State University Medical Center, Columbus, Ohio.

Todd Lucas (T)

Division of Public Health and Department of Epidemiology, College of Human Medicine, Michigan State University, East Lansing, Michigan.

Damodara Rao Mendu (DR)

Department of Pathology, Icahn School of Medicine at Mount Sinai, New York, New York.

Ann M Moormann (AM)

Department of Medicine, University of Massachusetts Chan Medical School, Worcester, Massachusetts.

Vel Murugan (V)

Virginia G Piper Center for Personalized Diagnostics, Arizona State University Biodesign Institute, Tempe, Arizona.

Nkemakonam C Okoye (NC)

Department of Pathology and Laboratory Medicine, Donald and Barbara Zucker School of Medicine at Hofstra/Northwell, Hempstead, New York.

Petraleigh Pantoja (P)

Unit of Comparative Medicine, University of Puerto Rico-Medical Sciences Campus, San Juan, Puerto Rico.

Anne F Payne (AF)

Wadsworth Center, New York State Department of Health, Albany, New York.

Jin Park (J)

Virginia G Piper Center for Personalized Diagnostics, Arizona State University Biodesign Institute, Tempe, Arizona.

Swetha Pinninti (S)

Department of Pediatrics, University of Alabama at Birmingham, Birmingham, Alabama.

Amelia K Pinto (AK)

Department of Molecular Microbiology & Immunology, Saint Louis University, Saint Louis, Missouri.

Nora Pisanic (N)

Department of Environmental Health and Engineering, Johns Hopkins Bloomberg School of Public Health, Baltimore, Maryland.

Ji Qiu (J)

Virginia G Piper Center for Personalized Diagnostics, Arizona State University Biodesign Institute, Tempe, Arizona.

Carlos A Sariol (CA)

Unit of Comparative Medicine, University of Puerto Rico-Medical Sciences Campus, San Juan, Puerto Rico.
Department of Internal Medicine, University of Puerto Rico-Medical Sciences Campus, San Juan, Puerto Rico.

Viviana Simon (V)

Department of Microbiology, Icahn School of Medicine at Mount Sinai, New York, New York.

Lusheng Song (L)

Virginia G Piper Center for Personalized Diagnostics, Arizona State University Biodesign Institute, Tempe, Arizona.

Tara L Steffen (TL)

Department of Molecular Microbiology & Immunology, Saint Louis University, Saint Louis, Missouri.

E Taylor Stone (ET)

Department of Molecular Microbiology & Immunology, Saint Louis University, Saint Louis, Missouri.

Linda M Styer (LM)

Wadsworth Center, New York State Department of Health, Albany, New York.
Department of Biomedical Sciences, School of Public Health, University at Albany, Albany, New York.

Mehul S Suthar (MS)

Center for Childhood Infections and Vaccines of Children's Healthcare Atlanta, Department of Pediatrics, Department of Microbiology and Immunology, Emory Vaccine Center, Yerkes National Primate Research Center, Emory University School of Medicine, Atlanta, Georgia.

Stefani N Thomas (SN)

Department of Laboratory Medicine and Pathology, University of Minnesota, Minneapolis, Minnesota.

Bharat Thyagarajan (B)

Department of Laboratory Medicine and Pathology, University of Minnesota, Minneapolis, Minnesota.

Ania Wajnberg (A)

Department of Medicine, Icahn School of Medicine at Mount Sinai, New York, New York.

Jennifer L Yates (JL)

Wadsworth Center, New York State Department of Health, Albany, New York.
Department of Biomedical Sciences, School of Public Health, University at Albany, Albany, New York.

Kimia Sobhani (K)

Department of Pathology and Laboratory Medicine, Cedars-Sinai Medical Center, Los Angeles, California.

Classifications MeSH