Single-cell transcriptomics reveals expression profiles of Trypanosoma brucei sexual stages.
Journal
PLoS pathogens
ISSN: 1553-7374
Titre abrégé: PLoS Pathog
Pays: United States
ID NLM: 101238921
Informations de publication
Date de publication:
03 2022
03 2022
Historique:
received:
01
11
2021
accepted:
06
02
2022
revised:
22
03
2022
pubmed:
8
3
2022
medline:
23
4
2022
entrez:
7
3
2022
Statut:
epublish
Résumé
Early diverging lineages such as trypanosomes can provide clues to the evolution of sexual reproduction in eukaryotes. In Trypanosoma brucei, the pathogen that causes Human African Trypanosomiasis, sexual reproduction occurs in the salivary glands of the insect host, but analysis of the molecular signatures that define these sexual forms is complicated because they mingle with more numerous, mitotically-dividing developmental stages. We used single-cell RNA-sequencing (scRNAseq) to profile 388 individual trypanosomes from midgut, proventriculus, and salivary glands of infected tsetse flies allowing us to identify tissue-specific cell types. Further investigation of salivary gland parasite transcriptomes revealed fine-scale changes in gene expression over a developmental progression from putative sexual forms through metacyclics expressing variant surface glycoprotein genes. The cluster of cells potentially containing sexual forms was characterized by high level transcription of the gamete fusion protein HAP2, together with an array of surface proteins and several genes of unknown function. We linked these expression patterns to distinct morphological forms using immunofluorescence assays and reporter gene expression to demonstrate that the kinetoplastid-conserved gene Tb927.10.12080 is exclusively expressed at high levels by meiotic intermediates and gametes. Further experiments are required to establish whether this protein, currently of unknown function, plays a role in gamete formation and/or fusion.
Identifiants
pubmed: 35255094
doi: 10.1371/journal.ppat.1010346
pii: PPATHOGENS-D-21-02201
pmc: PMC8939820
doi:
Types de publication
Journal Article
Research Support, Non-U.S. Gov't
Langues
eng
Sous-ensembles de citation
IM
Pagination
e1010346Subventions
Organisme : Biotechnology and Biological Sciences Research Council
ID : BB/R016437/1
Pays : United Kingdom
Organisme : Biotechnology and Biological Sciences Research Council
ID : BB/R010188/1
Pays : United Kingdom
Organisme : Wellcome Trust
ID : 206194/Z/17/Z
Pays : United Kingdom
Organisme : Wellcome Trust
ID : 220185/Z/20/Z
Pays : United Kingdom
Déclaration de conflit d'intérêts
The authors have declared that no competing interests exist.
Références
Nat Protoc. 2014 Jan;9(1):171-81
pubmed: 24385147
Nat Biotechnol. 2011 May 15;29(7):644-52
pubmed: 21572440
Commun Biol. 2021 May 11;4(1):555
pubmed: 33976359
Elife. 2018 Mar 27;7:
pubmed: 29580379
Nat Biotechnol. 2018 Jun;36(5):411-420
pubmed: 29608179
Science. 2019 Aug 23;365(6455):
pubmed: 31439762
Parasitology. 1999 May;118 ( Pt 5):469-78
pubmed: 10363280
Nat Protoc. 2013 Aug;8(8):1494-512
pubmed: 23845962
BMC Genomics. 2018 Apr 2;19(1):227
pubmed: 29606092
Mol Biochem Parasitol. 2014 Jun;195(1):59-73
pubmed: 24992042
BMC Genomics. 2018 Feb 13;19(1):140
pubmed: 29439658
Bioinformatics. 2015 Jan 15;31(2):166-9
pubmed: 25260700
Front Cell Infect Microbiol. 2021 Mar 01;11:604129
pubmed: 33732658
BMC Genomics. 2018 Jun 19;19(1):477
pubmed: 29914354
J Biol Chem. 2006 Sep 15;281(37):26832-46
pubmed: 16857679
Nat Commun. 2021 May 27;12(1):3196
pubmed: 34045457
Brief Bioinform. 2013 Mar;14(2):144-61
pubmed: 22908213
Nature. 2017 Nov 2;551(7678):95-99
pubmed: 29094698
Biochem J. 1997 Sep 1;326 ( Pt 2):415-23
pubmed: 9291113
Mol Microbiol. 2007 Jan;63(1):218-28
pubmed: 17229212
Nat Methods. 2017 May;14(5):483-486
pubmed: 28346451
Proc Natl Acad Sci U S A. 2011 Mar 1;108(9):3671-6
pubmed: 21321215
PLoS Pathog. 2021 Sep 20;17(9):e1009904
pubmed: 34543350
Nature. 2009 May 14;459(7244):213-7
pubmed: 19444208
PLoS Biol. 2020 May 4;18(5):e3000711
pubmed: 32365102
PLoS Negl Trop Dis. 2012;6(6):e1708
pubmed: 22724039
Mol Biochem Parasitol. 2002 Jan;119(1):87-95
pubmed: 11755189
Mol Biochem Parasitol. 2019 Jun;230:24-36
pubmed: 30550896
Nature. 2014 Jan 30;505(7485):681-685
pubmed: 24336212
J Protozool. 1977 May;24(2):325-9
pubmed: 881656
Proc Natl Acad Sci U S A. 2020 Feb 4;117(5):2613-2621
pubmed: 31964820
Sci Rep. 2018 Feb 1;8(1):2151
pubmed: 29391536
Nat Commun. 2019 Aug 14;10(1):3660
pubmed: 31413257
Nat Commun. 2021 Sep 6;12(1):5268
pubmed: 34489460
Genes Dev. 2000 Mar 1;14(5):615-26
pubmed: 10716949
PLoS Pathog. 2005 Nov;1(3):e22
pubmed: 16276404
Microbiology (Reading). 2001 Dec;147(Pt 12):3231-40
pubmed: 11739755
PLoS Biol. 2020 Jun 10;18(6):e3000741
pubmed: 32520929
Mol Microbiol. 2017 Oct;106(1):74-92
pubmed: 28742275
Mol Biochem Parasitol. 1997 Mar;85(1):99-112
pubmed: 9108552
PLoS Negl Trop Dis. 2021 Sep 20;15(9):e0009504
pubmed: 34543277
J Cell Sci. 2005 Jan 15;118(Pt 2):283-90
pubmed: 15654017
Trends Parasitol. 2021 Oct;37(10):850-852
pubmed: 34391665
Bioinformatics. 2017 Apr 15;33(8):1179-1186
pubmed: 28088763
PLoS One. 2016 Dec 21;11(12):e0168877
pubmed: 28002435
J Comput Biol. 2012 May;19(5):455-77
pubmed: 22506599
Nat Methods. 2020 Jun;17(6):615-620
pubmed: 32366989
Genome Biol Evol. 2014 Oct 05;6(10):2811-9
pubmed: 25287146
Protist. 2008 Jan;159(1):137-51
pubmed: 17931969
Genome Biol. 2016 Apr 27;17:75
pubmed: 27122128
Parasit Vectors. 2008 Feb 25;1(1):4
pubmed: 18298832
Nat Commun. 2020 Mar 5;11(1):1201
pubmed: 32139671
PLoS Negl Trop Dis. 2014 Apr 24;8(4):e2649
pubmed: 24763140
PLoS Pathog. 2022 Feb 9;18(2):e1010300
pubmed: 35139131
Science. 2012 Dec 7;338(6112):1352-3
pubmed: 23224556
Curr Biol. 2014 Jan 20;24(2):181-186
pubmed: 24388851
Mol Biochem Parasitol. 2015 Mar-Apr;200(1-2):1-4
pubmed: 25896436
Trends Parasitol. 2017 Feb;33(2):80-82
pubmed: 27863903
Eukaryot Cell. 2002 Dec;1(6):1000-9
pubmed: 12477800
J Mol Biol. 2001 Sep 28;312(4):597-607
pubmed: 11575917
Parasitology. 2021 Sep;148(10):1223-1236
pubmed: 33678213
Nat Methods. 2015 Apr;12(4):357-60
pubmed: 25751142
Bioinformatics. 2010 Mar 15;26(6):841-2
pubmed: 20110278
Mol Biochem Parasitol. 2006 Sep;149(1):1-9
pubmed: 16682088
Br Med Bull. 1985 Apr;41(2):105-14
pubmed: 3928017