Sequence and functional characterization of a public HIV-specific antibody clonotype.

Immunology Virology

Journal

iScience
ISSN: 2589-0042
Titre abrégé: iScience
Pays: United States
ID NLM: 101724038

Informations de publication

Date de publication:
21 Jan 2022
Historique:
received: 19 05 2021
revised: 30 08 2021
accepted: 01 12 2021
entrez: 5 1 2022
pubmed: 6 1 2022
medline: 6 1 2022
Statut: epublish

Résumé

Public antibody clonotypes shared among multiple individuals have been identified for several pathogens. However, little is known about the determinants of antibody "publicness". Here, we characterize the sequence and functional properties of antibodies from a public clonotype targeting the CD4 binding site on HIV-1 Env. Our results showed that HIV-1 specificity for the public antibodies studied here, comprising sequences from three individuals, was modulated by the V

Identifiants

pubmed: 34984325
doi: 10.1016/j.isci.2021.103564
pii: S2589-0042(21)01534-0
pmc: PMC8692997
doi:

Types de publication

Journal Article

Langues

eng

Pagination

103564

Subventions

Organisme : NIAID NIH HHS
ID : P30 AI110527
Pays : United States
Organisme : NIAID NIH HHS
ID : R01 AI131722
Pays : United States

Informations de copyright

© 2021 The Author(s).

Déclaration de conflit d'intérêts

I.S.G. is a co-founder of AbSeek Bio. I.S., L.M., and I.S.G. are listed as inventors on patents filed for the antibodies described here. The Georgiev laboratory at Vanderbilt University Medical Center has received unrelated funding from Takeda Pharmaceuticals.

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Auteurs

Amyn A Murji (AA)

Department of Pathology, Microbiology, and Immunology, Vanderbilt University Medical Center, Nashville, TN, USA.
Vanderbilt Vaccine Center, Vanderbilt University Medical Center, Nashville, TN 37232, USA.

Nagarajan Raju (N)

Department of Pathology, Microbiology, and Immunology, Vanderbilt University Medical Center, Nashville, TN, USA.
Vanderbilt Vaccine Center, Vanderbilt University Medical Center, Nashville, TN 37232, USA.

Juliana S Qin (JS)

Vanderbilt Vaccine Center, Vanderbilt University Medical Center, Nashville, TN 37232, USA.

Haajira Kaldine (H)

National Institute for Communicable Diseases of the National Health Laboratory Service, Johannesburg 2131, South Africa.
Antibody Immunity Research Unit, Faculty of Health Sciences, University of the Witwatersrand, Johannesburg 2000, South Africa.

Katarzyna Janowska (K)

Division of Structural Biology, Duke Human Vaccine Institute, Duke University School of Medicine, Durham, NC 27710, USA.
Department of Surgery, Duke University School of Medicine, Durham, NC 27710, USA.

Emilee Friedman Fechter (EF)

Vanderbilt Vaccine Center, Vanderbilt University Medical Center, Nashville, TN 37232, USA.

Rutendo Mapengo (R)

National Institute for Communicable Diseases of the National Health Laboratory Service, Johannesburg 2131, South Africa.
Antibody Immunity Research Unit, Faculty of Health Sciences, University of the Witwatersrand, Johannesburg 2000, South Africa.

Cathrine Scheepers (C)

National Institute for Communicable Diseases of the National Health Laboratory Service, Johannesburg 2131, South Africa.
Antibody Immunity Research Unit, Faculty of Health Sciences, University of the Witwatersrand, Johannesburg 2000, South Africa.

Ian Setliff (I)

Vanderbilt Vaccine Center, Vanderbilt University Medical Center, Nashville, TN 37232, USA.

Priyamvada Acharya (P)

Division of Structural Biology, Duke Human Vaccine Institute, Duke University School of Medicine, Durham, NC 27710, USA.
Department of Surgery, Duke University School of Medicine, Durham, NC 27710, USA.

Lynn Morris (L)

National Institute for Communicable Diseases of the National Health Laboratory Service, Johannesburg 2131, South Africa.
Antibody Immunity Research Unit, Faculty of Health Sciences, University of the Witwatersrand, Johannesburg 2000, South Africa.
Centre for the AIDS Programme of Research in South Africa (CAPRISA), University of KwaZulu-Natal, Durban 4041, South Africa.

Ivelin S Georgiev (IS)

Department of Pathology, Microbiology, and Immunology, Vanderbilt University Medical Center, Nashville, TN, USA.
Vanderbilt Vaccine Center, Vanderbilt University Medical Center, Nashville, TN 37232, USA.
Program in Computational Microbiology and Immunology, Vanderbilt University Medical Center, Nashville, TN 37232, USA.
Vanderbilt Institute for Infection, Immunology and Inflammation, Vanderbilt University Medical Center, Nashville, TN 37232, USA.
Department of Electrical Engineering and Computer Science, Vanderbilt University, Nashville, TN 37232, USA.
Center for Structural Biology, Vanderbilt University, Nashville, TN 37232, USA.

Classifications MeSH