Immunosuppressive mesenchymal stem cells aggregates incorporating hydrogel microspheres promote an in vitro invasion of cancer cells.
(CCL)5, chemokine (C–C motif) ligand
2D, two-dimensional
3D, three-dimensional
Anti-cancer drug screening
CAF, cancer-associated fibroblasts
Cancer invasion model
DDW, double-distilled water
DMEM, Dulbecco's modified Eagle's medium
ELISA, enzyme-linked immunosolvent assay
FCS, fetal calf serum
GM, gelatin hydrogel microspheres
Gelatin hydrogel microspheres
MEM, minimum essential medium
MMP, matrix metalloproteinase
MSC, mesenchymal stem cells
MSC2, MSC of an immunosuppressive phenotype
Mesenchymal stem cells
PBS, phosphate buffered-saline
PVA, poly (vinyl alcohol)
TAM, tumor-associated macrophages
Three-dimensional cell culture
Journal
Regenerative therapy
ISSN: 2352-3204
Titre abrégé: Regen Ther
Pays: Netherlands
ID NLM: 101709085
Informations de publication
Date de publication:
Dec 2021
Dec 2021
Historique:
received:
15
10
2021
revised:
16
11
2021
accepted:
24
11
2021
entrez:
3
1
2022
pubmed:
4
1
2022
medline:
4
1
2022
Statut:
epublish
Résumé
The objective of this study is to design a co-culture system of cancer cells and three-dimensional (3D) mesenchymal stem cells (MSC) aggregates for the in vitro evaluation of cancer invasion. First, the MSC of an immunosuppressive phenotype (MSC2) were prepared by the MSC stimulation of polyriboinosinic polyribocytidylic acid. By simple mixing MSC2 and gelatin hydrogel microspheres (GM) in a U-bottomed well of 96 well plates which had been pre-coated with poly (vinyl alcohol), 3D MSC2 aggregates incorporating GM were obtained. The amount of chemokine (C-C motif) ligand 5 (CCL5) secreted from the MSC2 aggregates incorporating GM. Finally, an invasion assay was performed to evaluate the cancer invasion rate by co-cultured cancer cells and the 3D MSC2 incorporating GM. The amount of CCL5 secreted for the 3D MSC2 aggregates incorporating GM was significantly higher than that of two-dimensional (2D) MSC, 2D MSC2, and 3D MSC aggregates incorporating GM. When MDA-MB-231 human breast cancer cells were co-cultured with the 3D MSC2 aggregates incorporating GM, the invasion rate of cancer cells was significantly high compared with that of 2D MSC or 2D MSC2 and 3D MSC aggregates incorporating GM. In addition, high secretion of matrix metalloproteinase-2 was observed for the 3D MSC2 aggregates/cancer cells system. It is concluded that the co-culture system of 3D MSC2 aggregates incorporating GM and cancer cells is promising to evaluate the invasion of cancer cells in vitro.
Identifiants
pubmed: 34977285
doi: 10.1016/j.reth.2021.11.006
pii: S2352-3204(21)00088-2
pmc: PMC8668441
doi:
Types de publication
Journal Article
Langues
eng
Pagination
516-522Informations de copyright
© 2021 The Japanese Society for Regenerative Medicine. Production and hosting by Elsevier B.V.
Déclaration de conflit d'intérêts
The authors declare no conflict of interest.
Références
Oncogene. 2011 Aug 4;30(31):3429-39
pubmed: 21399660
J Biosci Bioeng. 2019 Nov;128(5):606-612
pubmed: 31103425
Biomacromolecules. 2017 Dec 11;18(12):4373-4384
pubmed: 29040808
Tissue Eng Part B Rev. 2020 Apr;26(2):164-180
pubmed: 31910095
Sci Rep. 2017 Sep 4;7(1):10382
pubmed: 28871147
J Biotechnol. 2017 Mar 20;246:81-93
pubmed: 28131858
Biotechnol J. 2016 Nov;11(11):1452-1460
pubmed: 27617786
Adv Drug Deliv Rev. 1998 May 4;31(3):287-301
pubmed: 10837630
Biomaterials. 1998 Dec;19(23):2139-45
pubmed: 9884054
Adv Drug Deliv Rev. 2016 Feb 1;97:260-9
pubmed: 26658243
Oncotarget. 2016 Aug 30;7(35):56904-56914
pubmed: 27486877
Cancer Res. 2009 Apr 1;69(7):2996-3003
pubmed: 19318572
Int J Mol Sci. 2017 May 18;18(5):
pubmed: 28524079
J Cell Physiol. 2008 Jul;216(1):189-97
pubmed: 18264981
Cell. 2018 Nov 29;175(6):1607-1619.e15
pubmed: 30500539
Eur J Cancer. 2006 Apr;42(6):717-27
pubmed: 16520032
Biotechnol J. 2008 Oct;3(9-10):1172-84
pubmed: 18566957
Acta Biomater. 2019 Jun;91:195-208
pubmed: 31034948
Biomaterials. 1999 Nov;20(22):2169-75
pubmed: 10555085
Proc Natl Acad Sci U S A. 2012 Mar 13;109(11):4245-50
pubmed: 22371586
J Exp Med. 2013 Nov 18;210(12):2675-92
pubmed: 24190430
Cancers (Basel). 2021 Feb 23;13(4):
pubmed: 33672435
Sci Rep. 2015 Nov 20;5:16842
pubmed: 26585309
Nature. 2012 Nov 8;491(7423):254-8
pubmed: 23034650
Biotechnol Bioeng. 2002 Oct 5;80(1):73-83
pubmed: 12209788
Acta Biomater. 2018 Jun;73:236-249
pubmed: 29679778
Regen Ther. 2020 Mar 04;14:196-204
pubmed: 32154334
Biomaterials. 2012 Nov;33(33):8430-41
pubmed: 22940219
Int J Mol Sci. 2021 Aug 12;22(16):
pubmed: 34445363
Nat Protoc. 2020 Oct;15(10):3380-3409
pubmed: 32929210
Tissue Eng Part A. 2020 Dec;26(23-24):1272-1282
pubmed: 32434426
Mol Neurobiol. 2015 Feb;51(1):19-31
pubmed: 24961568
Sci Rep. 2020 Feb 3;10(1):1653
pubmed: 32015396
Biochim Biophys Acta. 2013 Dec;1836(2):321-35
pubmed: 24183942
Tissue Eng Part C Methods. 2019 Jun;25(6):344-352
pubmed: 31062648
Regen Ther. 2019 Jul 10;11:131-138
pubmed: 31338392
Regen Ther. 2018 Feb 04;8:29-37
pubmed: 30271863
Biomaterials. 2017 Jan;115:141-154
pubmed: 27889665
Dis Model Mech. 2019 Jul 29;12(7):
pubmed: 31383635
Tissue Eng Part C Methods. 2019 Dec;25(12):711-720
pubmed: 31621504
World J Stem Cells. 2019 Dec 26;11(12):1065-1083
pubmed: 31875869
Cancers (Basel). 2020 Sep 24;12(10):
pubmed: 32987868
J Biol Chem. 2013 Aug 2;288(31):22584-95
pubmed: 23775082
J Biol Chem. 2012 May 18;287(21):17016-17028
pubmed: 22453918
Regen Ther. 2021 Sep 25;18:372-383
pubmed: 34632010
Nat Rev Mol Cell Biol. 2020 Oct;21(10):571-584
pubmed: 32636524
Molecules. 2021 Nov 10;26(22):
pubmed: 34833885
Biomaterials. 2015 Jul;55:110-8
pubmed: 25934456
Biomaterials. 2000 Mar;21(5):489-99
pubmed: 10674814
Biomaterials. 2006 Mar;27(7):1061-70
pubmed: 16111746
Regen Ther. 2020 Jan 13;14:11-18
pubmed: 31970268
PLoS One. 2012;7(9):e45590
pubmed: 23029122
Regen Ther. 2017 Aug 04;7:34-44
pubmed: 30271850
Sci Rep. 2019 Jan 22;9(1):292
pubmed: 30670761
Adv Biosyst. 2020 Aug;4(8):e1900250
pubmed: 32686345
Biomaterials. 2014 Dec;35(38):10070-9
pubmed: 25282622
Tissue Eng Part A. 2013 Mar;19(5-6):669-84
pubmed: 23013450
Exp Biol Med (Maywood). 2012 Jan;237(1):38-49
pubmed: 22185919
Biotechnol Bioeng. 2019 Jan;116(1):206-226
pubmed: 30367820
PLoS One. 2010 Apr 26;5(4):e10088
pubmed: 20436665
Carcinogenesis. 2008 Nov;29(11):2227-35
pubmed: 18725385
Nat Rev Immunol. 2005 Dec;5(12):953-64
pubmed: 16322748
J Tissue Eng Regen Med. 2013 Oct;7(10):801-11
pubmed: 22438136
Acta Biomater. 2011 Jul;7(7):2797-803
pubmed: 21549223