Selective Detection of Penicillin G Antibiotic in Milk by Molecularly Imprinted Polymer-Based Plasmonic SPR Sensor.

Ag nanoparticles molecularly imprinted polymers penicillin G sensor surface plasmon resonance

Journal

Biomimetics (Basel, Switzerland)
ISSN: 2313-7673
Titre abrégé: Biomimetics (Basel)
Pays: Switzerland
ID NLM: 101719189

Informations de publication

Date de publication:
14 Dec 2021
Historique:
received: 12 11 2021
revised: 06 12 2021
accepted: 10 12 2021
entrez: 23 12 2021
pubmed: 24 12 2021
medline: 24 12 2021
Statut: epublish

Résumé

Molecularly imprinted polymer-based surface plasmon resonance sensor prepared using silver nanoparticles was designed for the selective recognition of Penicillin G (PEN-G) antibiotic from both aqueous solution and milk sample. PEN-G imprinted sensors (NpMIPs) SPR sensor was fabricated using poly (2-hydroxyethyl methacrylate-N-methacroyl-(L)-cysteine methyl ester)-silver nanoparticles-N-methacryloyl-L-phenylalanine methyl ester polymer by embedding silver nanoparticles (AgNPs) into the polymeric film structure. In addition, a non-imprinted (NpNIPs) SPR sensor was prepared by utilizing the same polymerization recipe without addition of the PEN-G template molecule to evaluate the imprinting effect. FTIR-ATR spectrophotometer, ellipsometer, contact angle measurements were used for the characterization of NpMIPs SPR sensors. The linear concentration range of 0.01-10 ng/mL PEN-G was studied for kinetic analyses. The augmenting effect of AgNPs used to increase the surface plasmon resonance signal response was examined using polymer-based PEN-G imprinted (MIPs) sensor without the addition of AgNPs. The antibiotic amount present in milk chosen as a real sample was measured by spiking PEN-G into the milk. According to the Scatchard, Langmuir, Freundlich and Langmuir-Freundlich adsorption models, the interaction mechanism was estimated to be compatible with the Langmuir model.

Identifiants

pubmed: 34940015
pii: biomimetics6040072
doi: 10.3390/biomimetics6040072
pmc: PMC8698653
pii:
doi:

Types de publication

Journal Article

Langues

eng

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Auteurs

Monireh Bakhshpour (M)

Department of Chemistry, Hacettepe University, Ankara 06800, Turkey.

Ilgım Göktürk (I)

Department of Chemistry, Hacettepe University, Ankara 06800, Turkey.

Nilay Bereli (N)

Department of Chemistry, Hacettepe University, Ankara 06800, Turkey.

Fatma Yılmaz (F)

Department of Chemistry Technology, Bolu Abant Izzet Baysal University, Bolu 14900, Turkey.

Adil Denizli (A)

Department of Chemistry, Hacettepe University, Ankara 06800, Turkey.

Classifications MeSH