Molecular Mechanism of Antioxidant and Anti-Inflammatory Effects of Omega-3 Fatty Acids in Perilla Seed Oil and Rosmarinic Acid Rich Fraction Extracted from Perilla Seed Meal on TNF-α Induced A549 Lung Adenocarcinoma Cells.
A549 Cells
Adenocarcinoma of Lung
/ metabolism
Anti-Inflammatory Agents
/ chemistry
Antioxidants
/ chemistry
Cinnamates
/ chemistry
Depsides
/ chemistry
Fatty Acids, Omega-3
/ chemistry
Humans
Lung Neoplasms
/ metabolism
Oxidative Stress
/ drug effects
Perilla
/ chemistry
Plant Oils
/ chemistry
alpha-Linolenic Acid
/ chemistry
Rosmarinic Acid
Perilla frutescens
inflammation
omega-3
perilla oil
perilla seed meal
rosmarinic acid
Journal
Molecules (Basel, Switzerland)
ISSN: 1420-3049
Titre abrégé: Molecules
Pays: Switzerland
ID NLM: 100964009
Informations de publication
Date de publication:
09 Nov 2021
09 Nov 2021
Historique:
received:
01
08
2021
revised:
04
11
2021
accepted:
05
11
2021
entrez:
27
11
2021
pubmed:
28
11
2021
medline:
15
12
2021
Statut:
epublish
Résumé
Industrially, after the removal of oil from perilla seeds (PS) by screw-type compression, the large quantities of residual perilla seed meal (PSM) becomes non-valuable waste. Therefore, to increase the health value and price of PS and PSM, we focused on the biological effects of perilla seed oil (PSO) and rosmarinic acid-rich fraction (RA-RF) extracted from PSM for their role in preventing oxidative stress and inflammation caused by TNF-α exposure in an A549 lung adenocarcinoma culture model. The A549 cells were pretreated with PSO or RA-RF and followed by TNF-α treatment. We found that PSO and RA-RF were not toxic to TNF-α-induced A549 cells. Both extracts significantly decreased the generation of reactive oxygen species (ROS) in this cell line. The mRNA expression levels of IL-1β, IL-6, IL-8, TNF-α, and COX-2 were significantly decreased by the treatment of PSO and RA-RF. The Western blot indicated that the expression of MnSOD, FOXO1, and NF-κB and phosphorylation of JNK were also significantly diminished by PSO and RA-RF treatment. The results demonstrated that PSO and RA-RF act as antioxidants to scavenge TNF-α induced ROS levels, resulting in decreased the expression of MnSOD, FOXO1, NF-κB and JNK signaling pathway in a human lung cell culture exposed to TNF-α.
Identifiants
pubmed: 34833849
pii: molecules26226757
doi: 10.3390/molecules26226757
pmc: PMC8622939
pii:
doi:
Substances chimiques
Anti-Inflammatory Agents
0
Antioxidants
0
Cinnamates
0
Depsides
0
Fatty Acids, Omega-3
0
Plant Oils
0
alpha-Linolenic Acid
0RBV727H71
perilla seed oil
68132-21-8
Types de publication
Journal Article
Langues
eng
Sous-ensembles de citation
IM
Subventions
Organisme : Thailand Research Fund (TRF) and Office of the Higher Education Commission (OHEC)
ID : MRG6180080
Organisme : Unit of Excellence University of Phayao
ID : FF64-UoE019
Organisme : University of Phayao research grant 2017
ID : RD60071
Références
J Glob Oncol. 2018 Aug;4:1-29
pubmed: 30192698
J Cell Biol. 2018 Jun 4;217(6):1915-1928
pubmed: 29669742
Adv Exp Med Biol. 2014;816:1-23
pubmed: 24818717
Exp Biol Med (Maywood). 2008 Jun;233(6):674-88
pubmed: 18408140
Nutrients. 2016 Mar 02;8(3):128
pubmed: 26950145
Cancer Metastasis Rev. 2015 Jun;34(2):319-31
pubmed: 26059063
Oncol Lett. 2020 Sep;20(3):2506-2515
pubmed: 32782570
J Chromatogr B Analyt Technol Biomed Life Sci. 2014 Feb 1;947-948:41-8
pubmed: 24381020
Int J Oncol. 2000 Jul;17(1):107-12
pubmed: 10853026
Diabetes. 2009 Nov;58(11):2624-33
pubmed: 19651810
Molecules. 2020 Feb 22;25(4):
pubmed: 32098445
Food Sci Nutr. 2019 Apr 23;7(5):1645-1655
pubmed: 31139377
Mol Cancer Res. 2013 Mar;11(3):261-71
pubmed: 23271813
Molecules. 2018 Dec 28;24(1):
pubmed: 30597896
J Pharmacol Sci. 2016 Oct;132(2):131-137
pubmed: 27707649
Prostaglandins Leukot Essent Fatty Acids. 2018 May;132:34-40
pubmed: 29599053
Biochem Biophys Res Commun. 2019 Mar 26;511(1):122-128
pubmed: 30771899
BMC Cancer. 2010 Jun 23;10:323
pubmed: 20573209
Food Chem Toxicol. 2017 Oct;108(Pt B):375-391
pubmed: 27890564
Curr Alzheimer Res. 2019;16(2):146-155
pubmed: 30543172
Regul Toxicol Pharmacol. 2019 Apr;103:229-236
pubmed: 30703409
Molecules. 2020 Jun 08;25(11):
pubmed: 32521713
Acta Pol Pharm. 2010 Nov-Dec;67(6):696-700
pubmed: 21229890
Biomed Pharmacother. 2002 Oct;56(8):365-79
pubmed: 12442909
Semin Cancer Biol. 2018 Jun;50:65-76
pubmed: 29309929
Biol Chem. 2014 Feb;395(2):203-30
pubmed: 24127541
Oncogene. 2006 Oct 30;25(51):6758-80
pubmed: 17072327
Molecules. 2016 Jun 13;21(6):
pubmed: 27304950
Front Physiol. 2018 May 17;9:477
pubmed: 29867535
Biochem Biophys Res Commun. 2009 Jan 9;378(2):290-5
pubmed: 19026986
Int J Biol Sci. 2017 Jul 6;13(7):815-827
pubmed: 28808415
Molecules. 2016 Aug 18;21(8):
pubmed: 27548124
Food Chem. 2014 Apr 1;148:367-72
pubmed: 24262570
Stem Cells Int. 2019 Oct 31;2019:2120453
pubmed: 31781234
Food Chem. 2014 Dec 1;164:150-7
pubmed: 24996318
BMC Public Health. 2018 Sep 24;18(1):1138
pubmed: 30249219
J Food Sci Technol. 2020 Apr;57(4):1258-1268
pubmed: 32180622
J Sci Food Agric. 2021 Mar 15;101(4):1419-1427
pubmed: 32833246
Neoplasia. 2021 Feb;23(2):189-196
pubmed: 33373873
Biomed Res Int. 2014;2014:925350
pubmed: 24864265
Int J Cardiol. 2013 Oct 3;168(3):2637-45
pubmed: 23587398
Biomed Res Int. 2019 Mar 4;2019:9514703
pubmed: 30949513
Front Immunol. 2018 Sep 27;9:2160
pubmed: 30319623
J Thorac Cardiovasc Surg. 1991 Dec;102(6):904-7
pubmed: 1960995
Lipids. 2008 Jun;43(6):499-506
pubmed: 18386091
Methods. 2001 Dec;25(4):402-8
pubmed: 11846609
Trends Cell Biol. 2016 Apr;26(4):249-261
pubmed: 26791157
J Med Food. 2020 Aug;23(8):818-826
pubmed: 32552354
EMBO J. 2004 Dec 8;23(24):4802-12
pubmed: 15538382
Food Sci Biotechnol. 2020 Sep 5;29(11):1553-1562
pubmed: 33088604
Molecules. 2019 Nov 13;24(22):
pubmed: 31766230
Cells. 2020 Apr 02;9(4):
pubmed: 32252279
Biosci Rep. 2020 May 29;40(5):
pubmed: 32426811
Curr Opin Pulm Med. 2009 Jul;15(4):303-7
pubmed: 19417670
Int J Mol Sci. 2019 Aug 14;20(16):
pubmed: 31416203
J Nutr Biochem. 2012 Dec;23(12):1609-16
pubmed: 22444500
Front Pharmacol. 2020 Feb 28;11:153
pubmed: 32184728
Antioxidants (Basel). 2021 Feb 17;10(2):
pubmed: 33671207
Foods. 2020 Apr 22;9(4):
pubmed: 32331363
Signal Transduct Target Ther. 2017;2:
pubmed: 29158945
Phytother Res. 2019 Aug;33(8):2064-2074
pubmed: 31141248
Int J Mol Sci. 2019 Oct 11;20(20):
pubmed: 31614433
J Tradit Chin Med. 2013 Apr;33(2):170-5
pubmed: 23789212
Nutr Hosp. 2011 Mar-Apr;26(2):323-9
pubmed: 21666970
Am J Physiol Gastrointest Liver Physiol. 2006 Apr;290(4):G583-9
pubmed: 16537970