Methodology to Quantify Collagen Subtypes and Crosslinks: Application in Minipig Cartilages.

Animals Chromatography, Liquid Collagen / metabolism Hyaline Cartilage Swine Swine, Miniature / metabolism Tandem Mass Spectrometry
cartilage collagen collagen crosslinks collagen subtype fibrocartilage mass spectrometry

Journal

Cartilage
ISSN: 1947-6043
Titre abrégé: Cartilage
Pays: United States
ID NLM: 101518378

Informations de publication

Date de publication:
12 2021
Historique:
pubmed: 27 11 2021
medline: 7 4 2022
entrez: 26 11 2021
Statut: ppublish

Résumé

This study develops assays to quantify collagen subtypes and crosslinks with liquid chromatography-mass spectrometry (LC-MS) and characterizes the cartilages in the Yucatan minipig. For collagen subtyping, liquid chromatography-tandem mass spectrometry (LC-MS/MS) analysis was performed on tissues digested in trypsin. For collagen crosslinks, LC-MS analysis was performed on hydrolysates. Samples were also examined histologically and with bottom-up proteomics. Ten cartilages (femoral condyle, femoral head, facet joint, floating rib, true rib, auricular cartilage, annulus fibrosus, 2 meniscus locations, and temporomandibular joint disc) were analyzed. The collagen subtyping assay quantified collagen types I and II. The collagen crosslinks assay quantified mature and immature crosslinks. Collagen subtyping revealed that collagen type I predominates in fibrocartilages and collagen type II in hyaline cartilages, as expected. Elastic cartilage and fibrocartilages had more mature collagen crosslink profiles than hyaline cartilages. Bottom-up proteomics revealed a spectrum of ratios between collagen types I and II, and quantified 42 proteins, including 24 collagen alpha-chains and 12 minor collagen types. The novel assays developed in this work are sensitive, inexpensive, and use a low operator time relative to other collagen analysis methods. Unlike the current collagen assays, these assays quantify collagen subtypes and crosslinks without an antibody-based approach or lengthy chromatography. They apply to any collagenous tissue, with broad applications in tissue characterization and tissue engineering. For example, a novel finding of this work was the presence of a large quantity of collagen type III in the white-white knee meniscus and a spectrum of hyaline and fibrous cartilages.

Identifiants

DOI: 10.1177/19476035211060508 PMID: 34823380 PMC: PMC8804780
pubmed: 34823380
doi: 10.1177/19476035211060508
pmc: PMC8804780
doi:

Substances chimiques

Collagen 9007-34-5

Types de publication

Journal Article Research Support, N.I.H., Extramural

Langues

eng

Sous-ensembles de citation

IM

Pagination

1742S-1754S

Subventions

Organisme : NIAMS NIH HHS
ID : R01 AR067821
Pays : United States
Organisme : NIAMS NIH HHS
ID : R01 AR071457
Pays : United States
Organisme : NIDCR NIH HHS
ID : R01 DE015038
Pays : United States

Références

Matrix Biol. 2020 Jan;85-86:47-67
pubmed: 31655293
Osteoarthritis Cartilage. 2015 Oct;23(10):1797-805
pubmed: 26033166
J Orthop Surg (Hong Kong). 2013 Aug;21(2):216-20
pubmed: 24014788
Eur Cell Mater. 2018 Jul 27;36:30-43
pubmed: 30051455
Ann Otol Rhinol Laryngol. 2017 Dec;126(12):819-828
pubmed: 29078705
Eur J Biochem. 1994 Sep 15;224(3):943-50
pubmed: 7925418
Brief Funct Genomic Proteomic. 2009 Mar;8(2):145-57
pubmed: 19279071
PLoS One. 2014 Nov 14;9(11):e112391
pubmed: 25397407
Cell Transplant. 2015;24(2):235-45
pubmed: 24380383
J Drugs Dermatol. 2009 Mar;8(3 Suppl):1-8
pubmed: 19288692
J Sep Sci. 2019 Apr;42(8):1482-1488
pubmed: 30680922
PLoS One. 2011;6(10):e26178
pubmed: 22022553
Biomed Res Int. 2013;2013:916530
pubmed: 23971049
J Surg Res. 2014 Jan;186(1):73-80
pubmed: 24035229
Connect Tissue Res. 1987;16(4):343-56
pubmed: 3132349
Biomaterials. 2011 Oct;32(30):7411-31
pubmed: 21764438
Sci Transl Med. 2018 Jun 20;10(446):
pubmed: 29925634
J Biol Chem. 1995 Aug 11;270(32):18865-70
pubmed: 7642541
Nat Biotechnol. 2008 Dec;26(12):1367-72
pubmed: 19029910
Sports Health. 2009 Nov;1(6):461-8
pubmed: 23015907
Biochem J. 1975 Dec;151(3):595-602
pubmed: 766752
Molecules. 2019 Nov 07;24(22):
pubmed: 31703345
Pathol Biol (Paris). 2005 Sep;53(7):430-42
pubmed: 16085121
Tissue Eng Part C Methods. 2017 Nov;23(11):700-709
pubmed: 28548559
J Biol Chem. 2012 Jun 1;287(23):18913-24
pubmed: 22493511
Biochem J. 1984 Jul 1;221(1):189-96
pubmed: 6380497
Adv Drug Deliv Rev. 2019 Jun;146:289-305
pubmed: 30605736
Biochim Biophys Acta. 1980 Dec 16;626(2):346-55
pubmed: 7213652
FEBS Lett. 1983 Jul 25;158(2):265-70
pubmed: 6688225
Biomed Pharmacother. 2019 Nov;119:109419
pubmed: 31563117
J Anat. 1980 Dec;131(Pt 4):611-20
pubmed: 7216901
Nat Rev Rheumatol. 2019 Sep;15(9):550-570
pubmed: 31296933
J Cosmet Dermatol. 2018 Feb;17(1):20-26
pubmed: 29144022
Tissue Eng. 2007 Sep;13(9):2195-205
pubmed: 17630876
Tumour Biol. 2014 Apr;35(4):2871-82
pubmed: 24338768
Tissue Eng Part C Methods. 2017 Apr;23(4):243-250
pubmed: 28406755
Nat Rev Mater. 2020 Oct;5(10):730-747
pubmed: 33996147
J Proteomics. 2014 Aug 28;108:269-83
pubmed: 24878426
Cartilage. 2020 Jan;11(1):71-76
pubmed: 29926745
PLoS One. 2018 Apr 10;13(4):e0195261
pubmed: 29634740

Auteurs

Benjamin J Bielajew (BJ)

Department of Biomedical Engineering, University of California, Irvine, Irvine, CA, USA.

Jerry C Hu (JC)

Department of Biomedical Engineering, University of California, Irvine, Irvine, CA, USA.

Kyriacos A Athanasiou (KA)

Department of Biomedical Engineering, University of California, Irvine, Irvine, CA, USA.
ORCID: 0000-0001-5387-8405

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Classifications MeSH

Recherchemedicale.com › Eucaryotes › Animaux › Methodology to Quantify Collagen Subtypes and...
Recherchemedicale.com › Techniques d'investigation › Techniques de chimie analytique › Chromatographie › Chromatographie en phase liquide › Methodology to Quantify Collagen Subtypes and...
Recherchemedicale.com › Acides aminés, peptides et protéines › Protéines › Scléroprotéines › Protéines de la matrice extracellulaire › Collagène › Methodology to Quantify Collagen Subtypes and...
Recherchemedicale.com › Tissus › Tissu conjonctif › Cartilage › Cartilage hyalin › Methodology to Quantify Collagen Subtypes and...
Recherchemedicale.com › Eucaryotes › Animaux › Chordés › Vertébrés › Mammifères › Eutheria › Artiodactyla › Suidae › Methodology to Quantify Collagen Subtypes and...
Recherchemedicale.com › Eucaryotes › Animaux › Chordés › Vertébrés › Mammifères › Eutheria › Artiodactyla › Suidae › Sus scrofa › Porc miniature › Methodology to Quantify Collagen Subtypes and...
Recherchemedicale.com › Techniques d'investigation › Techniques de chimie analytique › Spectrométrie de masse › Spectrométrie de masse en tandem › Methodology to Quantify Collagen Subtypes and...