Super-resolved 3D-STED microscopy identifies a layer-specific increase in excitatory synapses in the hippocampal CA1 region of Neuroligin-3 KO mice.
Animals
Autism Spectrum Disorder
/ diagnostic imaging
CA1 Region, Hippocampal
/ diagnostic imaging
Cell Adhesion Molecules, Neuronal
/ deficiency
Cognition
/ physiology
Disease Models, Animal
Gene Knockout Techniques
Homer Scaffolding Proteins
/ genetics
Male
Membrane Proteins
/ deficiency
Mice
Mice, Inbred C57BL
Mice, Knockout
Microscopy
/ instrumentation
Nerve Tissue Proteins
/ deficiency
Neuroimaging
/ instrumentation
Neurons
/ metabolism
Synapses
/ genetics
Synaptic Transmission
/ physiology
Autism spectrum disorder (ASD)
Neuroligin-3
Super-resolved stimulated emission depletion (STED) microscopy
Synapse
Journal
Biochemical and biophysical research communications
ISSN: 1090-2104
Titre abrégé: Biochem Biophys Res Commun
Pays: United States
ID NLM: 0372516
Informations de publication
Date de publication:
10 12 2021
10 12 2021
Historique:
received:
07
09
2021
accepted:
02
10
2021
pubmed:
30
10
2021
medline:
29
12
2021
entrez:
29
10
2021
Statut:
ppublish
Résumé
The chemical synapse is one type of cell-adhesion system that transmits information from a neuron to another neuron in the complex neuronal network in the brain. Synaptic transmission is the rate-limiting step during the information processing in the neuronal network and its plasticity is involved in cognitive functions. Thus, morphological and electrophysiological analyses of synapses are of particular importance in neuroscience research. In the current study, we applied super-resolved three-dimensional stimulated emission depletion (3D-STED) microscopy for the morphological analyses of synapses. This approach allowed us to estimate the precise number of excitatory and inhibitory synapses in the mouse hippocampal tissue. We discovered a region-specific increase in excitatory synapses in a model mouse of autism spectrum disorder, Neuroligin-3 KO, with this method. This type of analysis will open a new field in developmental neuroscience in the future.
Identifiants
pubmed: 34715405
pii: S0006-291X(21)01395-4
doi: 10.1016/j.bbrc.2021.10.003
pii:
doi:
Substances chimiques
Cell Adhesion Molecules, Neuronal
0
Homer Scaffolding Proteins
0
Membrane Proteins
0
Nerve Tissue Proteins
0
gephyrin
0
neuroligin 3
0
Types de publication
Journal Article
Research Support, Non-U.S. Gov't
Langues
eng
Sous-ensembles de citation
IM
Pagination
144-149Informations de copyright
Copyright © 2021 Elsevier Inc. All rights reserved.