Molecular Screening of Microorganisms Associated with Discolored Wood in Dead European Beech Trees Suffered from Extreme Drought Event Using Next Generation Sequencing.
Fagus sylvatica
Illumina sequencing
climate change
meta-barcoding
plant pathogenic bacteria
plant pathogenic fungi
Journal
Plants (Basel, Switzerland)
ISSN: 2223-7747
Titre abrégé: Plants (Basel)
Pays: Switzerland
ID NLM: 101596181
Informations de publication
Date de publication:
02 Oct 2021
02 Oct 2021
Historique:
received:
26
07
2021
revised:
18
09
2021
accepted:
22
09
2021
entrez:
23
10
2021
pubmed:
24
10
2021
medline:
24
10
2021
Statut:
epublish
Résumé
Drought events weaken trees and make them vulnerable to attacks by diverse plant pathogens. Here, we propose a molecular method for fast screening of microorganisms associated with European beech decline after an extreme drought period (2018) in a forest of Thuringia, Germany. We used Illumina sequencing with a recent bioinformatics approach based on DADA2 to identify archaeal, bacterial, and fungal ASVs (amplicon sequence variants) based on bacterial and archaeal 16S and fungal ITS genes. We show that symptomatic beech trees are associated with both bacterial and fungal plant pathogens. Although the plant pathogen sequences were detected in both discolored and non-discolored wood areas, they were highly enriched in the discolored wood areas. We show that almost each individual tree was associated with a different combination of pathogens.
Identifiants
pubmed: 34685901
pii: plants10102092
doi: 10.3390/plants10102092
pmc: PMC8537330
pii:
doi:
Types de publication
Journal Article
Langues
eng
Références
FEMS Microbiol Ecol. 2012 Dec;82(3):666-77
pubmed: 22738186
Front Microbiol. 2021 Aug 26;12:702467
pubmed: 34512579
New Phytol. 2002 Sep;155(3):449-460
pubmed: 33873318
Nat Methods. 2016 Jul;13(7):581-3
pubmed: 27214047
Front Microbiol. 2018 Sep 07;9:2140
pubmed: 30245681
Microorganisms. 2020 Jun 14;8(6):
pubmed: 32545893
Microb Ecol. 2022 Jul;84(1):90-105
pubmed: 34487212
J Environ Manage. 2014 Jun 15;139:109-19
pubmed: 24681650
Trends Genet. 2014 Sep;30(9):418-26
pubmed: 25108476
Gigascience. 2020 Nov 30;9(12):
pubmed: 33252655
Microbiome. 2021 Jan 22;9(1):19
pubmed: 33482913
Front Plant Sci. 2018 Nov 07;9:1563
pubmed: 30464766
ISME J. 2017 Oct 31;:
pubmed: 29087376
ISME J. 2017 Dec;11(12):2639-2643
pubmed: 28731476
Nucleic Acids Res. 2019 Jan 8;47(D1):D259-D264
pubmed: 30371820
Proc Natl Acad Sci U S A. 2011 Mar 15;108 Suppl 1:4516-22
pubmed: 20534432
MycoKeys. 2018 Nov 5;(41):119-120
pubmed: 30431624
FEMS Microbiol Ecol. 2020 Dec 2;96(12):
pubmed: 33038244
Microorganisms. 2021 Feb 12;9(2):
pubmed: 33673098
Microorganisms. 2019 Nov 20;7(12):
pubmed: 31756976
J Exp Bot. 2003 Jan;54(381):419-30
pubmed: 12493870
Mol Ecol. 2013 Nov;22(21):5271-7
pubmed: 24112409
mSphere. 2017 Mar 22;2(2):
pubmed: 28357413