Population structure, biogeography and transmissibility of Mycobacterium tuberculosis.


Journal

Nature communications
ISSN: 2041-1723
Titre abrégé: Nat Commun
Pays: England
ID NLM: 101528555

Informations de publication

Date de publication:
20 10 2021
Historique:
received: 14 10 2020
accepted: 06 09 2021
entrez: 21 10 2021
pubmed: 22 10 2021
medline: 20 11 2021
Statut: epublish

Résumé

Mycobacterium tuberculosis is a clonal pathogen proposed to have co-evolved with its human host for millennia, yet our understanding of its genomic diversity and biogeography remains incomplete. Here we use a combination of phylogenetics and dimensionality reduction to reevaluate the population structure of M. tuberculosis, providing an in-depth analysis of the ancient Indo-Oceanic Lineage 1 and the modern Central Asian Lineage 3, and expanding our understanding of Lineages 2 and 4. We assess sub-lineages using genomic sequences from 4939 pan-susceptible strains, and find 30 new genetically distinct clades that we validate in a dataset of 4645 independent isolates. We find a consistent geographically restricted or unrestricted pattern for 20 groups, including three groups of Lineage 1. The distribution of terminal branch lengths across the M. tuberculosis phylogeny supports the hypothesis of a higher transmissibility of Lineages 2 and 4, in comparison with Lineages 3 and 1, on a global scale. We define an expanded barcode of 95 single nucleotide substitutions that allows rapid identification of 69 M. tuberculosis sub-lineages and 26 additional internal groups. Our results paint a higher resolution picture of the M. tuberculosis phylogeny and biogeography.

Identifiants

pubmed: 34671035
doi: 10.1038/s41467-021-26248-1
pii: 10.1038/s41467-021-26248-1
pmc: PMC8528816
doi:

Types de publication

Journal Article

Langues

eng

Sous-ensembles de citation

IM

Pagination

6099

Subventions

Organisme : World Health Organization
ID : 001
Pays : International

Informations de copyright

© 2021. The Author(s).

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Auteurs

Luca Freschi (L)

Department of Biomedical Informatics, Harvard Medical School, Boston, MA, USA. l.freschi@gmail.com.

Roger Vargas (R)

Department of Biomedical Informatics, Harvard Medical School, Boston, MA, USA.
Department of Systems Biology, Harvard Medical School, Boston, MA, USA.

Ashaque Husain (A)

Directorate General of Health Services, Ministry of Health and Family Welfare, Dhaka, Bangladesh.

S M Mostofa Kamal (SMM)

Department of Pathology and Microbiology, National Institute of Diseases of the Chest and Hospital, Dhaka, Bangladesh.

Alena Skrahina (A)

Republican Scientific and Practical Centre for Pulmonology and Tuberculosis, Minsk, Belarus.

Sabira Tahseen (S)

National Reference Laboratory, National Tuberculosis Control Programme, Islamabad, Pakistan.

Nazir Ismail (N)

National Institute for Communicable Diseases, Sandringham, South Africa.
Department of Medical Microbiology, University of Pretoria, Pretoria, South Africa.

Anna Barbova (A)

Central Reference Laboratory on Tuberculosis Microbiological Diagnostics, Ministry of Health, Kiev, Ukraine.

Stefan Niemann (S)

Molecular and Experimental Mycobacteriology, Borstel Research Centre, Borstel, Germany.

Daniela Maria Cirillo (DM)

Emerging Bacterial Pathogens Unit, IRCCS San Raffaele Scientific Institute, Milan, Italy.

Anna S Dean (AS)

Global Tuberculosis Programme, World Health Organization, Geneva, Switzerland.

Matteo Zignol (M)

Global Tuberculosis Programme, World Health Organization, Geneva, Switzerland.

Maha Reda Farhat (MR)

Department of Biomedical Informatics, Harvard Medical School, Boston, MA, USA. maha_farhat@hms.harvard.edu.
Pulmonary and Critical Care Medicine, Massachusetts General Hospital, Boston, MA, USA. maha_farhat@hms.harvard.edu.

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