Infecting human hematopoietic stem and progenitor cells with SARS-CoV-2.
Cell Biology
Cell isolation
Cell-based Assays
Immunology
Microbiology
Microscopy
Stem Cells
Journal
STAR protocols
ISSN: 2666-1667
Titre abrégé: STAR Protoc
Pays: United States
ID NLM: 101769501
Informations de publication
Date de publication:
17 12 2021
17 12 2021
Historique:
pubmed:
12
10
2021
medline:
12
10
2021
entrez:
11
10
2021
Statut:
ppublish
Résumé
Determining how hematopoietic stem and progenitor cells (HSPCs) can be infected by viruses is necessary to understand and predict how the immune system will drive the host response. We present here a protocol to analyze the capacity of SARS-CoV-2 to infect different subsets of human HSPCs, inlcuding procedures for SARS-CoV-2 production and titration, isolation of human HSPCs from different sources (bone marrow, umbilical cord, or peripheral blood), and quantification of SARS-Cov-2 infection capacity by RT-qPCR and colony forming unit assay. For complete details on the use and execution of this protocol, please refer to Huerga Encabo et al. (2021).
Identifiants
pubmed: 34632411
doi: 10.1016/j.xpro.2021.100903
pii: S2666-1667(21)00609-2
pmc: PMC8492889
doi:
Types de publication
Journal Article
Research Support, Non-U.S. Gov't
Langues
eng
Pagination
100903Subventions
Organisme : Wellcome Trust
Pays : United Kingdom
Organisme : Cancer Research UK
ID : FC001045
Pays : United Kingdom
Organisme : Arthritis Research UK
ID : FC001045
Pays : United Kingdom
Organisme : Wellcome Trust
ID : FC001045
Pays : United Kingdom
Organisme : Wellcome Trust
ID : FC001045
Pays : United Kingdom
Informations de copyright
© 2021 The Authors.
Déclaration de conflit d'intérêts
The authors declare no competing interests.
Références
Bone Marrow Transplant. 2018 Mar;53(3):274-280
pubmed: 29269805
Stem Cell Reports. 2021 Mar 9;16(3):428-436
pubmed: 33581053