ROCK inhibitors modulate the physical properties and adipogenesis of 3D spheroids of human orbital fibroblasts in different manners.
ROCK
ROCK inhibitor
Rho kinase
human orbital fibroblasts (HOFs)
three‐dimensional (3D) tissue culture
Journal
FASEB bioAdvances
ISSN: 2573-9832
Titre abrégé: FASEB Bioadv
Pays: United States
ID NLM: 101733210
Informations de publication
Date de publication:
Oct 2021
Oct 2021
Historique:
received:
14
03
2021
revised:
10
07
2021
accepted:
12
07
2021
entrez:
11
10
2021
pubmed:
12
10
2021
medline:
12
10
2021
Statut:
epublish
Résumé
To elucidate the pharmacological effects of Rho-associated coiled-coil containing protein kinase inhibitors (ROCK-is), ripasudil (Rip), Y27632, and KD025, on human orbital fatty tissue, the human orbital fibroblasts (HOFs) were three-dimensional (3D) cultured for 12 days. The effects of ROCK-is on the physical properties of the 3D-cultured HOF spheroids, including their sizes and physical stiffness, their adipogenesis by lipid staining, and the mRNA expression of adipogenesis-related genes,
Identifiants
pubmed: 34632320
doi: 10.1096/fba.2021-00037
pii: FBA21265
pmc: PMC8493964
doi:
Types de publication
Journal Article
Langues
eng
Pagination
866-872Informations de copyright
© 2021 The Authors. FASEB BioAdvances published by Wiley Periodicals LLC on behalf of The Federation of American Societies for Experimental Biology.
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