Comparison of polynomial fitting versus single time point analysis of ECIS data for barrier assessment.
barrier function
electrical cell-substrate impedance sensing
Journal
Physiological reports
ISSN: 2051-817X
Titre abrégé: Physiol Rep
Pays: United States
ID NLM: 101607800
Informations de publication
Date de publication:
10 2021
10 2021
Historique:
received:
30
06
2021
accepted:
05
07
2021
entrez:
4
10
2021
pubmed:
5
10
2021
medline:
11
3
2022
Statut:
ppublish
Résumé
Electrical cell-substrate impedance sensing (ECIS) is an in vitro methodology for measuring the barrier integrity of a variety of cell types, including pulmonary endothelial cells. These experiments are frequently used for in vitro assessment of lung injury. The data derived from ECIS experiments consists of repeated measures of resistance across an endothelial monolayer. As such, these data reflect the dynamic changes in electrical resistance that occur over time. Currently methodologies for assessing ECIS data rely on single point assessments of barrier function, such as the maximal drop in trans-endothelial electrical resistance (TER
Identifiants
pubmed: 34605187
doi: 10.14814/phy2.14983
pmc: PMC8488550
doi:
Types de publication
Journal Article
Research Support, N.I.H., Extramural
Langues
eng
Sous-ensembles de citation
IM
Pagination
e14983Subventions
Organisme : NHLBI NIH HHS
ID : K08 HL132055
Pays : United States
Organisme : NHLBI NIH HHS
ID : R01 HL133413
Pays : United States
Informations de copyright
© 2021 The Authors. Physiological Reports published by Wiley Periodicals LLC on behalf of The Physiological Society and the American Physiological Society.
Références
Am J Physiol Lung Cell Mol Physiol. 2008 Sep;295(3):L379-99
pubmed: 18621912
Compr Physiol. 2011 Apr;1(2):835-82
pubmed: 23737205
Eur Respir J. 2021 Mar 11;57(3):
pubmed: 33154030
Proc Natl Acad Sci U S A. 1992 Sep 1;89(17):7919-23
pubmed: 1518814
Respir Res. 2017 Jun 2;18(1):116
pubmed: 28578669
Microvasc Res. 2009 Mar;77(2):174-86
pubmed: 19121327
Am J Respir Cell Mol Biol. 2004 Nov;31(5):517-27
pubmed: 15284075
J Biol Chem. 1997 May 16;272(20):13432-6
pubmed: 9148968
J Biol Chem. 2004 Jun 4;279(23):24692-700
pubmed: 15056655
Am J Physiol Lung Cell Mol Physiol. 2007 Apr;292(4):L924-35
pubmed: 17158600
Am J Respir Cell Mol Biol. 2014 May;50(5):932-41
pubmed: 24304496
J Exp Med. 1994 Jul 1;180(1):95-109
pubmed: 7911822
Am J Respir Cell Mol Biol. 2011 May;44(5):725-38
pubmed: 21531958
J Biol Chem. 2005 Apr 29;280(17):17286-93
pubmed: 15710622
Methods Enzymol. 2008;443:137-53
pubmed: 18772015
Am J Physiol Lung Cell Mol Physiol. 2018 Nov 1;315(5):L638-L644
pubmed: 30024307
Brain Res Bull. 2019 Mar;146:143-152
pubmed: 30508606
J Exp Med. 2009 Nov 23;206(12):2761-77
pubmed: 19917775
Am J Physiol Lung Cell Mol Physiol. 2019 Jun 1;316(6):L1118-L1126
pubmed: 30908935
J Appl Physiol (1985). 2001 Oct;91(4):1487-500
pubmed: 11568129
Am J Physiol Lung Cell Mol Physiol. 2001 Jun;280(6):L1168-78
pubmed: 11350795
Am J Physiol Lung Cell Mol Physiol. 2017 Jan 01;312(1):L143-L153
pubmed: 27913425