Investigation of the blood proteome in response to spinal cord injury in rodent models.
Journal
Spinal cord
ISSN: 1476-5624
Titre abrégé: Spinal Cord
Pays: England
ID NLM: 9609749
Informations de publication
Date de publication:
04 2022
04 2022
Historique:
received:
27
02
2020
accepted:
11
08
2021
revised:
11
08
2021
pubmed:
4
10
2021
medline:
12
4
2022
entrez:
3
10
2021
Statut:
ppublish
Résumé
Explanatory and mechanistic study. A better understanding of the 'whole-body' response following spinal cord injury (SCI) is needed to guide future research aimed at developing novel therapeutic interventions and identifying prognostic indicators for SCI. This study aimed to characterise the blood proteome following contusion or complete SCI compared to a sham injury in rat models. United Kingdom. Pooled blood samples from one and seven days after a contusion (serum; n = 5) or from 14 days and 112 days post-complete transection SCI (plasma; n = 8) and their sham-injured counterparts were subjected to independent iTRAQ nanoflow liquid chromatography tandem mass-spectrometry proteomic analyses. Pathway analyses of the proteins that were differentially abundant between SCI and their matched sham injured counterparts were completed to indicate biological pathways that may be changed in response to SCI. Eleven and 42 proteins were differentially abundant (≥±2.0 FC; p ≤ 0.05) between the contusion SCI and sham injured animals at 24 h and seven days post-injury, respectively. Seven and tweleve proteins were differentially abundant between complete and sham injured rats at 14 and 112 days post-injury, respectively. Acute-phase response signalling and Liver X Receptor/Retinoic X Receptor activation were identified as differentially regulated pathways in both models of SCI. We have utilised longitudinal preclinical SCI models to provide an insight into the blood proteome changes that result following SCI and to highlight a number of biological pathways of interest for future studies.
Identifiants
pubmed: 34601498
doi: 10.1038/s41393-021-00692-8
pii: 10.1038/s41393-021-00692-8
pmc: PMC8989679
doi:
Substances chimiques
Proteome
0
Types de publication
Journal Article
Langues
eng
Sous-ensembles de citation
IM
Pagination
320-325Subventions
Organisme : Wellcome Trust
Pays : United Kingdom
Organisme : Wellcome Trust
ID : 094476/Z/10/Z
Pays : United Kingdom
Informations de copyright
© 2021. Crown.
Références
Nucleic Acids Res. 2016 Jan 4;44(D1):D447-56
pubmed: 26527722
Biomater Sci. 2020 Jul 7;8(13):3611-3627
pubmed: 32515439
J Neurosci. 2005 Feb 2;25(5):1169-78
pubmed: 15689553
Spinal Cord. 2014 Aug;52(8):588-95
pubmed: 24912546
J Zhejiang Univ Sci B. 2005 Nov;6(11):1045-56
pubmed: 16252337
Lancet. 2011 Mar 19;377(9770):972-4
pubmed: 21377200
J Neurosurg. 1976 Apr;44(4):429-34
pubmed: 1255233
Mol Cell Neurosci. 2015 Nov;69:12-21
pubmed: 26370173
Int J Cell Biol. 2016;2016:9259646
pubmed: 28083070
J Neurotrauma. 2021 Feb;38(3):301-308
pubmed: 32703074
J Neurotrauma. 2016 Aug 1;33(15):1416-21
pubmed: 26825180
Exp Neurol. 2020 Jun;328:113273
pubmed: 32142803
J Vis Exp. 2014 Jul 27;(89):e50641
pubmed: 25145787
Neural Regen Res. 2014 Nov 15;9(22):2008-12
pubmed: 25598784
Spinal Cord. 2017 Feb;55(2):114-125
pubmed: 27995945
J Neurotrauma. 2020 Feb 1;37(3):466-480
pubmed: 31310157