Magnetic Nanoprobes for Spatio-Mechanical Manipulation in Single Cells.
iron oxide nanoparticles
magnetoferritin
magnetogenetics
remote particle manipulation
Journal
Nanomaterials (Basel, Switzerland)
ISSN: 2079-4991
Titre abrégé: Nanomaterials (Basel)
Pays: Switzerland
ID NLM: 101610216
Informations de publication
Date de publication:
31 Aug 2021
31 Aug 2021
Historique:
received:
30
07
2021
revised:
25
08
2021
accepted:
27
08
2021
entrez:
28
9
2021
pubmed:
29
9
2021
medline:
29
9
2021
Statut:
epublish
Résumé
Magnetic nanoparticles (MNPs) are widely known as valuable agents for biomedical applications. Recently, MNPs were further suggested to be used for a remote and non-invasive manipulation, where their spatial redistribution or force response in a magnetic field provides a fine-tunable stimulus to a cell. Here, we investigated the properties of two different MNPs and assessed their suitability for spatio-mechanical manipulations: semisynthetic magnetoferritin nanoparticles and fully synthetic 'nanoflower'-shaped iron oxide nanoparticles. As well as confirming their monodispersity in terms of structure, surface potential, and magnetic response, we monitored the MNP performance in a living cell environment using fluorescence microscopy and asserted their biocompatibility. We then demonstrated facilitated spatial redistribution of magnetoferritin compared to 'nanoflower'-NPs after microinjection, and a higher magnetic force response of these NPs compared to magnetoferritin inside a cell. Our remote manipulation assays present these tailored magnetic materials as suitable agents for applications in magnetogenetics, biomedicine, or nanomaterial research.
Identifiants
pubmed: 34578584
pii: nano11092267
doi: 10.3390/nano11092267
pmc: PMC8471295
pii:
doi:
Types de publication
Journal Article
Langues
eng
Subventions
Organisme : Volkswagen Foundation
ID : Freigeist Fellowship (Aktenzeichen: 94195)
Organisme : Deutsche Forschungsgemeinschaft
ID : INST 208/749-1 FUGG
Organisme : Fonds der Chemischen Industrie
ID : n.a.
Organisme : Deutsche Forschungsgemeinschaft
ID : SFB1208 (ID267205415)
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