Fluorescence Anisotropy as a Temperature-Sensing Molecular Probe Using Fluorescein.
fluorescein
fluorescence anisotropy
fluorescence polarization
microfluidics
temperature imaging
thermal molecular probe
Journal
Micromachines
ISSN: 2072-666X
Titre abrégé: Micromachines (Basel)
Pays: Switzerland
ID NLM: 101640903
Informations de publication
Date de publication:
15 Sep 2021
15 Sep 2021
Historique:
received:
29
07
2021
revised:
08
09
2021
accepted:
12
09
2021
entrez:
28
9
2021
pubmed:
29
9
2021
medline:
29
9
2021
Statut:
epublish
Résumé
Fluorescence anisotropy, a technique to study the folding state of proteins or affinity of ligands, is used in this present work as a temperature sensor, to measure the microfluidic temperature field, by adding fluorophore in the liquid. Fluorescein was used as a temperature-sensing probe, while glycerol-aq. ammonia solution was used as a working fluid. Fluorescence anisotropy of fluorescein was measured by varying various parameters. Apart from this, a comparison of fluorescence anisotropy and fluorescence intensity is also performed to demonstrate the validity of anisotropy to be applied in a microfluidic field with non-uniform liquid thickness. Viscosity dependence and temperature dependence on the anisotropy are also clarified; the results indicate an appropriate selection of relation between molecule size and viscosity is important to obtain a large temperature coefficient in anisotropy. Furthermore, a practical calibration procedure of the apparatus constant is proposed. In addition, the potential of temperature imaging is confirmed by the measurement of temperature distribution under focused laser heating.
Identifiants
pubmed: 34577751
pii: mi12091109
doi: 10.3390/mi12091109
pmc: PMC8469510
pii:
doi:
Types de publication
Journal Article
Langues
eng
Subventions
Organisme : Japan Science and Technology Agency
ID : JPMJCR1712
Organisme : Japan Society for the Promotion of Science
ID : 19H02084
Organisme : Japan Society for the Promotion of Science
ID : 19K21936
Organisme : Japan Society for the Promotion of Science
ID : 20K22400
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