Destruction mechanisms of ozone over SARS-CoV-2.
Coronavirus Papain-Like Proteases
/ chemistry
Coronavirus RNA-Dependent RNA Polymerase
/ chemistry
Humans
Microscopy, Electron, Transmission
Ozone
/ pharmacology
Protein Structure, Secondary
/ drug effects
Protein Structure, Tertiary
/ drug effects
SARS-CoV-2
/ chemistry
Time Factors
Viral Envelope
/ chemistry
Viral Regulatory and Accessory Proteins
/ chemistry
Zinc
/ chemistry
COVID-19 Drug Treatment
Journal
Scientific reports
ISSN: 2045-2322
Titre abrégé: Sci Rep
Pays: England
ID NLM: 101563288
Informations de publication
Date de publication:
22 09 2021
22 09 2021
Historique:
received:
01
12
2020
accepted:
23
08
2021
entrez:
23
9
2021
pubmed:
24
9
2021
medline:
9
10
2021
Statut:
epublish
Résumé
In this pandemic SARS-CoV-2 crisis, any attempt to contain and eliminate the virus will also stop its spread and consequently decrease the risk of severe illness and death. While ozone treatment has been suggested as an effective disinfection process, no precise mechanism of action has been previously reported. This study aimed to further investigate the effect of ozone treatment on SARS-CoV-2. Therefore, virus collected from nasopharyngeal and oropharyngeal swab and sputum samples from symptomatic patients was exposed to ozone for different exposure times. The virus morphology and structure were monitored and analyzed through Atomic Force Microscopy (AFM), Transmission Electron Microscopy (TEM), Atomic Absorption Spectroscopy (AAS), and ATR-FTIR. The obtained results showed that ozone treatment not only unsettles the virus morphology but also alters the virus proteins' structure and conformation through amino acid disturbance and Zn ion release from the virus non-structural proteins. These results could provide a clearer pathway for virus elimination and therapeutics preparation.
Identifiants
pubmed: 34552128
doi: 10.1038/s41598-021-97860-w
pii: 10.1038/s41598-021-97860-w
pmc: PMC8458291
doi:
Substances chimiques
NSP10 protein, SARS-CoV-2
0
Viral Regulatory and Accessory Proteins
0
Ozone
66H7ZZK23N
Coronavirus RNA-Dependent RNA Polymerase
EC 2.7.7.48
NSP12 protein, SARS-CoV-2
EC 2.7.7.48
Coronavirus Papain-Like Proteases
EC 3.4.22.2
papain-like protease, SARS-CoV-2
EC 3.4.22.2
Zinc
J41CSQ7QDS
Types de publication
Journal Article
Research Support, Non-U.S. Gov't
Langues
eng
Sous-ensembles de citation
IM
Pagination
18851Informations de copyright
© 2021. The Author(s).
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