Changes in serum biomarkers of inflammation in bovine besnoitiosis.


Journal

Parasites & vectors
ISSN: 1756-3305
Titre abrégé: Parasit Vectors
Pays: England
ID NLM: 101462774

Informations de publication

Date de publication:
22 Sep 2021
Historique:
received: 02 04 2021
accepted: 02 09 2021
entrez: 23 9 2021
pubmed: 24 9 2021
medline: 15 12 2021
Statut: epublish

Résumé

Acute and chronic besnoitiosis in extensive natural-service herds can have relevant effects in the health of bulls and negative consequences in their productive performance. Recent progress has been made in order to elucidate the pathogenesis of this disease. In this context, the study of biomarkers of inflammation in serum would contribute to gaining knowledge about the physiopathology of bovine besnoitiosis. Serological biomarkers could help in early diagnosis and prognosis, as seropositive bulls may have mild or severe testicular lesions. Herein, we have investigated the diagnostic and/or prognostic value of a panel of serum (serological) biomarkers related to inflammation, including total protein, globulin and albumin, haptoglobin (Hp), adenosine deaminase (ADA) paraoxonase-1 (PON-1) and acetylcholinesterase (AChE) in naturally and experimentally B. besnoiti-infected males classified according to different clinical phases of the disease (acute, chronic and subclinical besnoitiosis). Results showed a similar response pattern in these biomarkers for naturally and experimentally infected cattle, with a few relevant variations. Most significant changes occurred during the acute phase of infection, although significant changes in a few biomarkers were also observed during the chronic infection. Haptoglobin, albumin, PON-1 and ADA were identified as the biomarkers that showed changes of higher magnitude in the acute phase of the infection, whereas high total protein and globulin values were found in chronically infected cattle. We have described the changes of a panel of inflammatory biomarkers of acute and chronic bovine besnoitiosis. In summary, several biomarkers with promising diagnostic value have been identified. The biomarkers associated with acute infection are related to previously reported molecular biomarkers in testicular parenchyma of infected bulls and could help in the diagnosis of early infections and complement results from specific immunoglobulin M (IgM) detection.

Sections du résumé

BACKGROUND BACKGROUND
Acute and chronic besnoitiosis in extensive natural-service herds can have relevant effects in the health of bulls and negative consequences in their productive performance. Recent progress has been made in order to elucidate the pathogenesis of this disease. In this context, the study of biomarkers of inflammation in serum would contribute to gaining knowledge about the physiopathology of bovine besnoitiosis. Serological biomarkers could help in early diagnosis and prognosis, as seropositive bulls may have mild or severe testicular lesions.
METHODS METHODS
Herein, we have investigated the diagnostic and/or prognostic value of a panel of serum (serological) biomarkers related to inflammation, including total protein, globulin and albumin, haptoglobin (Hp), adenosine deaminase (ADA) paraoxonase-1 (PON-1) and acetylcholinesterase (AChE) in naturally and experimentally B. besnoiti-infected males classified according to different clinical phases of the disease (acute, chronic and subclinical besnoitiosis).
RESULTS RESULTS
Results showed a similar response pattern in these biomarkers for naturally and experimentally infected cattle, with a few relevant variations. Most significant changes occurred during the acute phase of infection, although significant changes in a few biomarkers were also observed during the chronic infection. Haptoglobin, albumin, PON-1 and ADA were identified as the biomarkers that showed changes of higher magnitude in the acute phase of the infection, whereas high total protein and globulin values were found in chronically infected cattle. We have described the changes of a panel of inflammatory biomarkers of acute and chronic bovine besnoitiosis.
CONCLUSIONS CONCLUSIONS
In summary, several biomarkers with promising diagnostic value have been identified. The biomarkers associated with acute infection are related to previously reported molecular biomarkers in testicular parenchyma of infected bulls and could help in the diagnosis of early infections and complement results from specific immunoglobulin M (IgM) detection.

Identifiants

pubmed: 34551803
doi: 10.1186/s13071-021-04991-0
pii: 10.1186/s13071-021-04991-0
pmc: PMC8459460
doi:

Substances chimiques

Biomarkers 0
Globulins 0
Haptoglobins 0
Acetylcholinesterase EC 3.1.1.7
Aryldialkylphosphatase EC 3.1.8.1
Adenosine Deaminase EC 3.5.4.4

Types de publication

Journal Article

Langues

eng

Sous-ensembles de citation

IM

Pagination

488

Subventions

Organisme : Ministerio de Economía y Competitividad
ID : AGL-2016-75202-R and PID2019-103960RB-I00
Organisme : Community of Madrid
ID : PLATESA P2018/ BAA-4370

Informations de copyright

© 2021. The Author(s).

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Auteurs

David González-Barrio (D)

Animal Health Department, Faculty of Veterinary Sciences, SALUVET, Complutense University of Madrid, Ciudad Universitaria s/n, 28040, Madrid, Spain. davigo20@ucm.es.

Ana Huertas-López (A)

Interdisciplinary Laboratory of Clinical Analysis (Interlab-UMU), Regional Campus of International Excellence Mare Nostrum, Espinardo, 30100, University of Murcia, Murcia, Spain.

Carlos Diezma-Díaz (C)

Animal Health Department, Faculty of Veterinary Sciences, SALUVET, Complutense University of Madrid, Ciudad Universitaria s/n, 28040, Madrid, Spain.

Ignacio Ferre (I)

Animal Health Department, Faculty of Veterinary Sciences, SALUVET, Complutense University of Madrid, Ciudad Universitaria s/n, 28040, Madrid, Spain.

José Joaquín Cerón (JJ)

Interdisciplinary Laboratory of Clinical Analysis (Interlab-UMU), Regional Campus of International Excellence Mare Nostrum, Espinardo, 30100, University of Murcia, Murcia, Spain.

Luis Miguel Ortega-Mora (LM)

Animal Health Department, Faculty of Veterinary Sciences, SALUVET, Complutense University of Madrid, Ciudad Universitaria s/n, 28040, Madrid, Spain.

Gema Álvarez-García (G)

Animal Health Department, Faculty of Veterinary Sciences, SALUVET, Complutense University of Madrid, Ciudad Universitaria s/n, 28040, Madrid, Spain.

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Classifications MeSH