Genome and transcriptome assemblies of the kuruma shrimp, Marsupenaeus japonicus.

Marsupenaeus japonicus Penaeus japonicus genome annotation genome sequencing kuruma prawn penaeid shrimp penaeidae transcriptome assembly

Journal

G3 (Bethesda, Md.)
ISSN: 2160-1836
Titre abrégé: G3 (Bethesda)
Pays: England
ID NLM: 101566598

Informations de publication

Date de publication:
19 10 2021
Historique:
received: 07 04 2021
accepted: 18 07 2021
pubmed: 14 9 2021
medline: 8 1 2022
entrez: 13 9 2021
Statut: ppublish

Résumé

The kuruma shrimp Marsupenaeus japonicus (order Decapoda, family Penaeidae) is an economically important crustacean that occurs in shallow, warm seas across the Indo-Pacific. Here, using a combination of Illumina and Oxford Nanopore Technologies platforms, we produced a draft genome assembly of M. japonicus (1.70 Gbp; 18,210 scaffolds; scaffold N50 = 234.9 kbp; 34.38% GC, 93.4% BUSCO completeness) and a complete mitochondrial genome sequence (15,969 bp). As with other penaeid shrimp genomes, the M. japonicus genome is extremely rich in simple repeats, which occupies 27.4% of the assembly. A total of 26,381 protein-coding gene models (94.7% BUSCO completeness) were predicted, of which 18,005 genes (68.2%) were assigned functional description by at least one method. We also produced an Illumina-based transcriptome shotgun assembly (40,991 entries; 93.0% BUSCO completeness) and a PacBio Iso-Seq transcriptome assembly (25,415 entries; 67.5% BUSCO completeness). We envision that the M. japonicus genome and transcriptome assemblies will serve as useful resources for the basic research, fisheries management, and breeding programs of M. japonicus.

Identifiants

pubmed: 34515781
pii: 6342415
doi: 10.1093/g3journal/jkab268
pmc: PMC8527471
pii:
doi:

Banques de données

figshare
['10.25387/g3.15043521']

Types de publication

Journal Article Research Support, Non-U.S. Gov't

Langues

eng

Sous-ensembles de citation

IM

Informations de copyright

© The Author(s) 2021. Published by Oxford University Press on behalf of Genetics Society of America.

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Auteurs

Satoshi Kawato (S)

Laboratory of Genome Science, Tokyo University of Marine Science and Technology, Tokyo 108-8477, Japan.

Koki Nishitsuji (K)

Marine Genomics Unit, Okinawa Institute of Science and Technology Graduate University, Okinawa 904-0495, Japan.

Asuka Arimoto (A)

Marine Genomics Unit, Okinawa Institute of Science and Technology Graduate University, Okinawa 904-0495, Japan.

Kanako Hisata (K)

Marine Genomics Unit, Okinawa Institute of Science and Technology Graduate University, Okinawa 904-0495, Japan.

Mayumi Kawamitsu (M)

DNA Sequencing Section, Okinawa Institute of Science and Technology Graduate University, Okinawa 904-0495, Japan.

Reiko Nozaki (R)

Laboratory of Genome Science, Tokyo University of Marine Science and Technology, Tokyo 108-8477, Japan.

Hidehiro Kondo (H)

Laboratory of Genome Science, Tokyo University of Marine Science and Technology, Tokyo 108-8477, Japan.

Chuya Shinzato (C)

Atmosphere and Ocean Research Institute, The University of Tokyo, Chiba 277-0882, Japan.

Tsuyoshi Ohira (T)

Faculty of Science, Department of Biological Sciences, Kanagawa University, Kanagawa 221-8686, Japan.

Noriyuki Satoh (N)

Marine Genomics Unit, Okinawa Institute of Science and Technology Graduate University, Okinawa 904-0495, Japan.

Eiichi Shoguchi (E)

Marine Genomics Unit, Okinawa Institute of Science and Technology Graduate University, Okinawa 904-0495, Japan.

Ikuo Hirono (I)

Laboratory of Genome Science, Tokyo University of Marine Science and Technology, Tokyo 108-8477, Japan.

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Classifications MeSH