SARS-CoV-2 Infection in Dogs and Cats from Southern Germany and Northern Italy during the First Wave of the COVID-19 Pandemic.
RT-qPCR
SARS-CoV-2
antibody
domestic animals
one health
prevalence
serology
surveillance
zoonosis
Journal
Viruses
ISSN: 1999-4915
Titre abrégé: Viruses
Pays: Switzerland
ID NLM: 101509722
Informations de publication
Date de publication:
26 07 2021
26 07 2021
Historique:
received:
30
06
2021
revised:
22
07
2021
accepted:
23
07
2021
entrez:
28
8
2021
pubmed:
29
8
2021
medline:
11
9
2021
Statut:
epublish
Résumé
Severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) has affected millions of people globally since its first detection in late 2019. Besides humans, cats and, to some extent, dogs were shown to be susceptible to SARS-CoV-2, highlighting the need for surveillance in a One Health context. Seven veterinary clinics from regions with high incidences of coronavirus disease (COVID-19) were recruited during the early pandemic (March to July 2020) for the screening of patients. A total of 2257 oropharyngeal and nasal swab specimen from 877 dogs and 260 cats (including 18 animals from COVID-19-affected households and 92 animals with signs of respiratory disease) were analyzed for the presence of SARS-CoV-2 RNA using reverse transcriptase real-time polymerase chain reaction (RT-qPCR) targeting the viral envelope (E) and RNA dependent RNA polymerase (RdRp) genes. One oropharyngeal swab from an Italian cat, living in a COVID-19-affected household in Piedmont, tested positive in RT-qPCR (1/260; 0.38%, 95% CI: 0.01-2.1%), and SARS-CoV-2 infection of the animal was serologically confirmed six months later. One oropharyngeal swab from a dog was potentially positive (1/877; 0.1%, 95% CI: 0.002-0.63%), but the result was not confirmed in a reference laboratory. Analyses of convenience sera from 118 animals identified one dog (1/94; 1.1%; 95% CI: 0.02-5.7%) from Lombardy, but no cats (0/24), as positive for anti-SARS-CoV-2 receptor binding domain (RBD) antibodies and neutralizing activity. These findings support the hypothesis that the prevalence of SARS-CoV-2 infection in pet cat and dog populations, and hence, the risk of zoonotic transmission to veterinary staff, was low during the first wave of the pandemic, even in hotspot areas.
Identifiants
pubmed: 34452319
pii: v13081453
doi: 10.3390/v13081453
pmc: PMC8402904
pii:
doi:
Substances chimiques
RNA, Viral
0
Types de publication
Journal Article
Research Support, Non-U.S. Gov't
Langues
eng
Sous-ensembles de citation
IM
Subventions
Organisme : NIAID NIH HHS
ID : HHSN272201400008C
Pays : United States
Organisme : Universität Zürich
ID : G-53420-01-01
Organisme : Bundesamt für Lebensmittelsicherheit und Veterinärwesen
ID : 0714001572
Références
Emerg Infect Dis. 2020 Dec;26(12):3069-3071
pubmed: 32788033
Emerg Infect Dis. 2021 Jul;27(7):1811-1820
pubmed: 34152956
Vaccines (Basel). 2020 Dec 17;8(4):
pubmed: 33348613
Pathogens. 2020 Jul 22;9(8):
pubmed: 32707796
Emerg Infect Dis. 2021 May;27(5):1362-1370
pubmed: 33900184
Pathogens. 2021 Feb 23;10(2):
pubmed: 33672421
Transbound Emerg Dis. 2021 Jul;68(4):1767-1773
pubmed: 33191649
Nat Biotechnol. 2020 Sep;38(9):1073-1078
pubmed: 32704169
BMC Vet Res. 2017 May 25;13(1):138
pubmed: 28545521
Vet Microbiol. 2006 Jan 10;112(1):11-21
pubmed: 16303261
J Clin Microbiol. 2021 Jan 21;59(2):
pubmed: 33139421
Lett Appl Microbiol. 2020 May;70(5):342-348
pubmed: 32060933
Zoonoses Public Health. 2021 Feb;68(1):67-68
pubmed: 33230915
BMC Vet Res. 2015 Nov 13;11:282
pubmed: 26566897
Viruses. 2021 May 19;13(5):
pubmed: 34069453
J Virol. 1998 May;72(5):4508-14
pubmed: 9557750
Euro Surveill. 2020 Jan;25(3):
pubmed: 31992387
Emerg Infect Dis. 2020 Dec;26(12):3071-3074
pubmed: 32938527
Nature. 2020 Oct;586(7831):776-778
pubmed: 32408337
Nat Commun. 2020 May 4;11(1):2251
pubmed: 32366817
J Infect Dis. 2021 Apr 23;223(8):1313-1321
pubmed: 33605423
Viruses. 2019 Oct 17;11(10):
pubmed: 31627345
Viruses. 2021 Jan 26;13(2):
pubmed: 33530620
N Engl J Med. 2020 Aug 6;383(6):592-594
pubmed: 32402157
Int J Biol Sci. 2020 Mar 15;16(10):1686-1697
pubmed: 32226286
Vet Pathol. 2014 Mar;51(2):505-26
pubmed: 24569616
Nat Commun. 2020 Dec 4;11(1):6231
pubmed: 33277505
Emerg Microbes Infect. 2020 Dec;9(1):2322-2332
pubmed: 33028154
J Infect Dis. 2021 Apr 23;223(8):1309-1312
pubmed: 33605418
Viruses. 2021 Mar 23;13(3):
pubmed: 33806922
Science. 2020 May 29;368(6494):1016-1020
pubmed: 32269068
Nat Microbiol. 2020 Apr;5(4):536-544
pubmed: 32123347
Heliyon. 2021 Jan;7(1):e05850
pubmed: 33392409
Vet Rec. 2021 Apr;188(8):e247
pubmed: 33890314
PLoS One. 2021 Mar 25;16(3):e0248578
pubmed: 33765012
J Small Anim Pract. 2021 May;62(5):336-342
pubmed: 33521974
Emerg Infect Dis. 2020 Jul;26(7):1478-1488
pubmed: 32267220
One Health. 2020 Dec;10:100164
pubmed: 32904469
Vet Clin North Am Small Anim Pract. 2011 Nov;41(6):1121-32
pubmed: 22041207
One Health. 2021 Jun;11:100192
pubmed: 33169106
Proc Natl Acad Sci U S A. 2020 Oct 20;117(42):26382-26388
pubmed: 32994343
Lancet Reg Health Eur. 2021 May;4:100056
pubmed: 33997830
Viruses. 2019 Oct 29;11(11):
pubmed: 31671816
Infect Dis Poverty. 2020 Apr 28;9(1):45
pubmed: 32345362
Viruses. 2021 Mar 17;13(3):
pubmed: 33802899
Transbound Emerg Dis. 2021 Jul;68(4):1850-1867
pubmed: 33091230