SARS-CoV-2 RNA and antibody detection in breast milk from a prospective multicentre study in Spain.
Adult
Antibodies, Viral
/ analysis
COVID-19
/ epidemiology
Coronavirus Envelope Proteins
/ analysis
Coronavirus Nucleocapsid Proteins
/ analysis
Female
Humans
Immunoglobulins
/ analysis
Longitudinal Studies
Milk, Human
/ immunology
Phosphoproteins
/ analysis
Prospective Studies
RNA, Viral
/ analysis
SARS-CoV-2
Spain
COVID-19
global health
microbiology
neonatology
Journal
Archives of disease in childhood. Fetal and neonatal edition
ISSN: 1468-2052
Titre abrégé: Arch Dis Child Fetal Neonatal Ed
Pays: England
ID NLM: 9501297
Informations de publication
Date de publication:
Mar 2022
Mar 2022
Historique:
received:
16
05
2021
accepted:
12
07
2021
pubmed:
22
8
2021
medline:
23
2
2022
entrez:
21
8
2021
Statut:
ppublish
Résumé
To develop and validate a specific protocol for SARS-CoV-2 detection in breast milk matrix and to determine the impact of maternal SARS-CoV-2 infection on the presence, concentration and persistence of specific SARS-CoV-2 antibodies. This is a prospective, multicentre longitudinal study (April-December 2020) in 60 mothers with SARS-CoV-2 infection and/or who have recovered from COVID-19. A control group of 13 women before the pandemic were also included. Seven health centres from different provinces in Spain. Presence of SARS-CoV-2 RNA in breast milk, targeting the N1 region of the nucleocapsid gene and the envelope (E) gene; presence and levels of SARS-CoV-2-specific immunoglobulins (Igs)-IgA, IgG and IgM-in breast milk samples from patients with COVID-19. All breast milk samples showed negative results for presence of SARS-CoV-2 RNA. We observed high intraindividual and interindividual variability in the antibody response to the receptor-binding domain of the SARS-CoV-2 spike protein for each of the three isotypes IgA, IgM and IgG. Main Protease (MPro) domain antibodies were also detected in milk. 82.9% (58 of 70) of milk samples were positive for at least one of the three antibody isotypes, with 52.9% of these positive for all three Igs. Positivity rate for IgA was relatively stable over time (65.2%-87.5%), whereas it raised continuously for IgG (from 47.8% for the first 10 days to 87.5% from day 41 up to day 206 post-PCR confirmation). Our study confirms the safety of breast feeding and highlights the relevance of virus-specific SARS-CoV-2 antibody transfer. This study provides crucial data to support official breastfeeding recommendations based on scientific evidence.
Identifiants
pubmed: 34417223
pii: archdischild-2021-322463
doi: 10.1136/archdischild-2021-322463
pmc: PMC8384494
doi:
Substances chimiques
Antibodies, Viral
0
Coronavirus Envelope Proteins
0
Coronavirus Nucleocapsid Proteins
0
Immunoglobulins
0
Phosphoproteins
0
RNA, Viral
0
envelope protein, SARS-CoV-2
0
nucleocapsid phosphoprotein, SARS-CoV-2
0
Banques de données
ClinicalTrials.gov
['NCT04768244']
Types de publication
Journal Article
Multicenter Study
Langues
eng
Sous-ensembles de citation
IM
Pagination
216-221Investigateurs
Elena Crehuá-Gaudiza
(E)
Javier Estañ-Capell
(J)
Asuncion Obiol
(A)
Reyes Balanza
(R)
Álvaro Solaz-García
(Á)
Inmaculada Lara-Cantón
(I)
Cristina Garcia
(C)
María Ríos Barnés
(MR)
Sara Ruiz
(S)
Marta Fabre
(M)
Federico García-García
(F)
Maria José Rodríguez-Lagunas
(MJ)
Karla Río-Aigé
(K)
Informations de copyright
© Author(s) (or their employer(s)) 2022. Re-use permitted under CC BY-NC. No commercial re-use. See rights and permissions. Published by BMJ.
Déclaration de conflit d'intérêts
Competing interests: None declared.
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