Kinetics of platelet adhesion to a fibrinogen-coated surface in whole blood under flow conditions.
Blood Platelets
/ metabolism
Fibrinogen
/ metabolism
Healthy Volunteers
Humans
Kinetics
Platelet Adhesiveness
Platelet Aggregation
Platelet Aggregation Inhibitors
/ pharmacology
Platelet Glycoprotein GPIIb-IIIa Complex
/ antagonists & inhibitors
Platelet Glycoprotein GPIb-IX Complex
/ antagonists & inhibitors
fibrinogen-coated surface
flow conditions
platelet adhesion
recording of a scattered laser light signal from a fibrinogen-covered surface
whole blood
Journal
Journal of clinical laboratory analysis
ISSN: 1098-2825
Titre abrégé: J Clin Lab Anal
Pays: United States
ID NLM: 8801384
Informations de publication
Date de publication:
Sep 2021
Sep 2021
Historique:
revised:
19
07
2021
received:
15
04
2021
accepted:
24
07
2021
pubmed:
5
8
2021
medline:
27
1
2022
entrez:
4
8
2021
Statut:
ppublish
Résumé
To test a novel method of assessment of platelet adhesion to a fibrinogen-coated surface in whole blood under flow conditions. We developed a fluidic device that mimics blood flow in vessels. The method of detection of platelet adhesion is based on recording of a scattered laser light signal from a fibrinogen-covered surface. Testing was performed in platelet-rich plasma (PRP) and whole blood of healthy volunteers. Control measurements were performed, followed by tests with inhibition of platelet GPIIa/IIIb and GPIb receptors. Then, the same testing sequence was performed in whole blood of persons with autoimmune thrombocytopenia and type 3 von Willebrand disease. The change in intensity of scattered light was 2.7 (2.4; 4.1) times higher in whole blood (0.2 ± 0.08V, n = 7) than in PRP (0.05 ± 0.02 V, n = 7), p < 0.01. The blocking of GP IIb/IIIa receptors decreased the intensity of scattered light to 8.5 (6.5;12)%; the blocking of GPIb receptors decreased it to 34 (23;58)%, p < 0.01. In the whole blood of a person with autoimmune thrombocytopenia, the inhibition of GPIb receptors decreased platelet adhesion, but no effect was observed in type 3 von Willebrand disease. Inhibition of platelet GPIIb/IIIa receptors alone or combined inhibition of GPIb and GPIIb/IIIa receptors resulted in almost total suppression of adhesion in both cases. Our system effectively registers platelet adhesion to a fibrinogen-coated surface under controlled-flow conditions and may successfully be applied to the investigation of platelet adhesion kinetics.
Identifiants
pubmed: 34347925
doi: 10.1002/jcla.23939
pmc: PMC8418503
doi:
Substances chimiques
Platelet Aggregation Inhibitors
0
Platelet Glycoprotein GPIIb-IIIa Complex
0
Platelet Glycoprotein GPIb-IX Complex
0
Fibrinogen
9001-32-5
Types de publication
Journal Article
Langues
eng
Sous-ensembles de citation
IM
Pagination
e23939Subventions
Organisme : Russian Science Foundation
ID : 21-15-00029
Informations de copyright
© 2021 The Authors. Journal of Clinical Laboratory Analysis published by Wiley Periodicals LLC.
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