Probing an Ixodes ricinus salivary gland yeast surface display with tick-exposed human sera to identify novel candidates for an anti-tick vaccine.
Animals
Antigens
/ blood
Borrelia burgdorferi
/ isolation & purification
Cattle
Cell Surface Display Techniques
/ methods
Female
Humans
Immunization
Ixodes
/ immunology
Lyme Disease
/ blood
Male
Peptide Fragments
/ immunology
Peptide Library
Rabbits
Saccharomyces cerevisiae
Salivary Glands
/ immunology
Salivary Proteins and Peptides
/ immunology
Tick Bites
/ immunology
Tick Infestations
/ immunology
Journal
Scientific reports
ISSN: 2045-2322
Titre abrégé: Sci Rep
Pays: England
ID NLM: 101563288
Informations de publication
Date de publication:
03 08 2021
03 08 2021
Historique:
received:
05
02
2021
accepted:
28
05
2021
entrez:
4
8
2021
pubmed:
5
8
2021
medline:
10
11
2021
Statut:
epublish
Résumé
In Europe, Ixodes ricinus is the most important vector of human infectious diseases, most notably Lyme borreliosis and tick-borne encephalitis virus. Multiple non-natural hosts of I. ricinus have shown to develop immunity after repeated tick bites. Tick immunity has also been shown to impair B. burgdorferi transmission. Most interestingly, multiple tick bites reduced the likelihood of contracting Lyme borreliosis in humans. A vaccine that mimics tick immunity could therefore potentially prevent Lyme borreliosis in humans. A yeast surface display library (YSD) of nymphal I. ricinus salivary gland genes expressed at 24, 48 and 72 h into tick feeding was constructed and probed with antibodies from humans repeatedly bitten by ticks, identifying twelve immunoreactive tick salivary gland proteins (TSGPs). From these, three proteins were selected for vaccination studies. An exploratory vaccination study in cattle showed an anti-tick effect when all three antigens were combined. However, immunization of rabbits did not provide equivalent levels of protection. Our results show that YSD is a powerful tool to identify immunodominant antigens in humans exposed to tick bites, yet vaccination with the three selected TSGPs did not provide protection in the present form. Future efforts will focus on exploring the biological functions of these proteins, consider alternative systems for recombinant protein generation and vaccination platforms and assess the potential of the other identified immunogenic TSGPs.
Identifiants
pubmed: 34344917
doi: 10.1038/s41598-021-92538-9
pii: 10.1038/s41598-021-92538-9
pmc: PMC8333314
doi:
Substances chimiques
Antigens
0
Peptide Fragments
0
Peptide Library
0
Salivary Proteins and Peptides
0
Types de publication
Journal Article
Research Support, Non-U.S. Gov't
Langues
eng
Sous-ensembles de citation
IM
Pagination
15745Informations de copyright
© 2021. The Author(s).
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