DEHT is a suitable plasticizer option for phthalate-free storage of irradiated red blood cells.
DEHP
DEHT
irradiation
phthalate
plasticizer
red blood cells
Journal
Vox sanguinis
ISSN: 1423-0410
Titre abrégé: Vox Sang
Pays: England
ID NLM: 0413606
Informations de publication
Date de publication:
Feb 2022
Feb 2022
Historique:
revised:
20
06
2021
received:
08
03
2021
accepted:
20
06
2021
pubmed:
17
7
2021
medline:
12
2
2022
entrez:
16
7
2021
Statut:
ppublish
Résumé
Due to increasing concerns about possible endocrine-disrupting properties, the use of the plasticizer di(2-ethylhexyl) phthalate (DEHP) will be banned in future blood storage. Di(2-ethylhexyl) terephthalate (DEHT) provides sufficient red blood cell (RBC) quality during conventional blood bank storage. It is important that a new plasticizer also maintains acceptable quality during exposure to high cell stress, such as irradiation, which is commonly used to prevent graft-versus-host disease. A total of 59 RBC units were collected and processed in polyvinyl chloride (PVC)-DEHT or PVC-DEHP blood bags combined with either saline-adenine-glucose-mannitol (SAGM) or phosphate-adenine-glucose-guanosine-saline-mannitol (PAGGSM) additive solution. All units were X-ray irradiated on day 2 post-collection. Sampling for assessment of parameters of storage lesion was performed on day 2 pre-irradiation and day 14 and 28 post-irradiation. Though irradiation increased cell stress, DEHT/PAGGSM and current common European preference DEHP/SAGM were equally affected up to 14 days post-irradiation for all measured parameters. At day 28, haemolysis and microvesicle count were slightly increased in DEHT, whereas extracellular potassium ions, glucose, lactate, pH, mean corpuscular volume and microvesicle phosphatidylserine remained unaffected by plasticizer choice throughout storage. No individual unit exceeded 0.8% haemolysis, not even in DEHT/SAGM, the combination overall most affected by irradiation. Of the four combinations, membrane stability was least impacted in DEHP/PAGGSM. We demonstrate that DEHT is a suitable plasticizer for storage of RBCs after X-ray irradiation cell stress. This strengthens the option of DEHT as a viable non-phthalate substitute for DEHP.
Sections du résumé
BACKGROUND AND OBJECTIVES
OBJECTIVE
Due to increasing concerns about possible endocrine-disrupting properties, the use of the plasticizer di(2-ethylhexyl) phthalate (DEHP) will be banned in future blood storage. Di(2-ethylhexyl) terephthalate (DEHT) provides sufficient red blood cell (RBC) quality during conventional blood bank storage. It is important that a new plasticizer also maintains acceptable quality during exposure to high cell stress, such as irradiation, which is commonly used to prevent graft-versus-host disease.
MATERIALS AND METHODS
METHODS
A total of 59 RBC units were collected and processed in polyvinyl chloride (PVC)-DEHT or PVC-DEHP blood bags combined with either saline-adenine-glucose-mannitol (SAGM) or phosphate-adenine-glucose-guanosine-saline-mannitol (PAGGSM) additive solution. All units were X-ray irradiated on day 2 post-collection. Sampling for assessment of parameters of storage lesion was performed on day 2 pre-irradiation and day 14 and 28 post-irradiation.
RESULTS
RESULTS
Though irradiation increased cell stress, DEHT/PAGGSM and current common European preference DEHP/SAGM were equally affected up to 14 days post-irradiation for all measured parameters. At day 28, haemolysis and microvesicle count were slightly increased in DEHT, whereas extracellular potassium ions, glucose, lactate, pH, mean corpuscular volume and microvesicle phosphatidylserine remained unaffected by plasticizer choice throughout storage. No individual unit exceeded 0.8% haemolysis, not even in DEHT/SAGM, the combination overall most affected by irradiation. Of the four combinations, membrane stability was least impacted in DEHP/PAGGSM.
CONCLUSION
CONCLUSIONS
We demonstrate that DEHT is a suitable plasticizer for storage of RBCs after X-ray irradiation cell stress. This strengthens the option of DEHT as a viable non-phthalate substitute for DEHP.
Substances chimiques
Phthalic Acids
0
Plasticizers
0
phthalic acid
6O7F7IX66E
Polyvinyl Chloride
9002-86-2
Diethylhexyl Phthalate
C42K0PH13C
Types de publication
Journal Article
Langues
eng
Sous-ensembles de citation
IM
Pagination
193-200Informations de copyright
© 2021 The Authors. Vox Sanguinis published by John Wiley & Sons Ltd on behalf of International Society of Blood Transfusion.
Références
Hess JR. Red cell changes during storage. Transfus Apher Sci. 2010;43:51-9.
Rubin O, Canellini G, Delobel J, Lion N, Tissot JD. Red blood cell microparticles: clinical relevance. Transfus Med Hemother. 2012;39:342-7.
Council of Europe. Guide to the preparation, use and quality assurance of blood components. 20th ed. Strasbourg, France: Council of Europe Publishing; 2020.
Sowemimo-Coker SO. Red blood cell hemolysis during processing. Transfus Med Rev. 2002;16:46-60.
Hansen AL, Kurach JD, Turner TR, Jenkins C, Busch MP, Norris PJ, et al. The effect of processing method on the in vitro characteristics of red blood cell products. Vox Sang. 2015;108:350-8.
de Korte D, Thibault L, Handke W, Harm SK, Morrison A, Fitzpatrick A, et al. Timing of gamma irradiation and blood donor sex influences in vitro characteristics of red blood cells. Transfusion. 2018;58:917-26.
Bashir S, Naik F, Cardigan R, Thomas S. Effect of X-irradiation on the quality of red cell concentrates. Vox Sang. 2011;101:200-7.
Kopolovic I, Ostro J, Tsubota H, Lin Y, Cserti-Gazdewich CM, Messner HA, et al. A systematic review of transfusion-associated graft-versus-host disease. Blood. 2015;126:406-14.
Moroff G, Luban NL. Prevention of transfusion-associated graft-versus-host disease. Transfusion. 1992;32:102-3.
Moreira OC, Oliveira VH, Benedicto LB, Nogueira CM, Mignaco JA, Fontes CFL, et al. Effects of gamma-irradiation on the membrane ATPases of human erythrocytes from transfusional blood concentrates. Ann Hematol. 2008;87:113-9.
Antosik A, Czubak K, Gajek A, Marczak A, Glowacki R, Borowczyk K, et al. Influence of pre-storage irradiation on the oxidative stress markers, membrane integrity, size and shape of the cold stored red blood cells. Transfus Med Hemother. 2015;42:140-8.
Anand AJ, Dzik WH, Imam A, Sadrzadeh SM. Radiation-induced red cell damage: role of reactive oxygen species. Transfusion. 1997;37:160-5.
Serrano K, Chen D, Hansen AL, Levin E, Turner TR, Kurach JDR, et al. The effect of timing of gamma-irradiation on hemolysis and potassium release in leukoreduced red cell concentrates stored in SAGM. Vox Sang. 2014;106:379-81.
Qadri SM, Chen D, Schubert P, Devine DV, Sheffield WP. Early γ-irradiation and subsequent storage of red cells in SAG-M additive solution potentiate energy imbalance, microvesiculation and susceptibility to stress-induced apoptotic cell death. Vox Sang. 2017;112:480-3.
Zhang Z, Shen Y, Li B, Li N. Cardiac arrest triggered by hyperkalemia during red blood cell transfusion: a case report and literature review. Transfus Med. 2017;27:384-6.
Hall TL, Barnes A, Miller JR, Bethencourt DM, Nestor L. Neonatal mortality following transfusion of red cells with high plasma potassium levels. Transfusion. 1993;33:606-9.
Svensk Förening för Transfusionsmedicin.: Handbok för Blodcentraler. KITM, 12 Mar 2015. 2015. https://transfusion.se/app/uploads/2020/04/blodkomponenter-framstallning-och-anvandning.pdf. Accessed 16 Feb 2021.
Foukaneli T, Kerr P, Bolton-Maggs PHB, Cardigan R, Coles A, Gennery A, et al. Guidelines on the use of irradiated blood components. Br J Haematol. 2020;191:704-24.
Rock G, Tocchi M, Ganz PR, Tackaberry ES. Incorporation of plasticizer into red cells during storage. Transfusion. 1984;24:493-8.
Horowitz B, Stryker MH, Waldman AA, Woods KR, Gass JD, Drago J. Stabilization of red blood cells by the plasticizer, diethylhexylphthalate. Vox Sang. 1985;48:150-5.
European Union. Regulation (EU) 2017/745 of the European Parliament and of the Council of 5 April 2017 on medical devices. Official J. 2017;L117:96.
van der Meer PF, Reesink HW, Panzer S, Wong J, Ismay S, Keller A, et al. Should DEHP be eliminated in blood bags? Vox Sang. 2014;106:176-95.
Larsson L, Sandgren P, Ohlsson S, Derving J, Friis-Christensen T, Daggert F, et al. Non-phthalate plasticizer DEHT preserves adequate blood component quality during storage in PVC blood bags. Vox Sang. 2021;116:60-70.
Graminske S, Puca K, Schmidt A, Brooks S, Boerner A, Heldke S, et al. In vitro evaluation of di(2-ethylhexyl)terephthalate-plasticized polyvinyl chloride blood bags for red blood cell storage in AS-1 and PAGGSM additive solutions. Transfusion. 2018;58:1100-7.
Larsson L, Larsson S, Derving J, Watz E, Uhlin M. A novel protocol for cryopreservation of paediatric red blood cell units allows increased availability of rare blood types. Vox Sang. 2019;114:711-20.
Gulliksson H, Meinke S, Ravizza A, Larsson L, Hoglund P. Storage of red blood cells in a novel polyolefin blood container: a pilot in vitro study. Vox Sang. 2017;112:33-9.
Daleke DL. Regulation of transbilayer plasma membrane phospholipid asymmetry. J Lipid Res. 2003;44:233-42.
Willekens FL, Werre JM, Kruijt JK, Roerdinkholder-Stoelwinder B, Groenen-Döpp YAM, van den Bos AG, et al. Liver Kupffer cells rapidly remove red blood cell-derived vesicles from the circulation by scavenger receptors. Blood. 2005;105:2141-5.
Almizraq R, Tchir JD, Holovati JL, Acker JP. Storage of red blood cells affects membrane composition, microvesiculation, and in vitro quality. Transfusion. 2013;53:2258-67.
Almizraq RJ, Holovati JL, Acker JP. Characteristics of extracellular vesicles in red blood concentrates change with storage time and blood manufacturing method. Transfus Med Hemother. 2018;45:185-93.
D'Alessandro A, Culp-Hill R, Reisz JA, Anderson M, Fu X, Nemkov T, et al. Heterogeneity of blood processing and storage additives in different centers impacts stored red blood cell metabolism as much as storage time: lessons from REDS-III-omics. Transfusion. 2019;59:89-100.
Shih AW, Apelseth TO, Cardigan R, Marks DC, Bégué S, Greinacher A, et al. Not all red cell concentrate units are equivalent: international survey of processing and in vitro quality data. Vox Sang. 2019;114:783-94.
Hess JR. An update on solutions for red cell storage. Vox Sang. 2006;91:13-9.
Lagerberg JW, Korsten H, Van Der Meer PF, De Korte D. Prevention of red cell storage lesion: a comparison of five different additive solutions. Blood Transfus. 2017;15:456-62.
Zehnder L, Schulzki T, Goede JS, Hayes J, Reinhart WH. Erythrocyte storage in hypertonic (SAGM) or isotonic (PAGGSM) conservation medium: influence on cell properties. Vox Sang. 2008;95:280-7.