Nandrolone and estradiol biomarkers identification in bovine urine applying a liquid chromatography high-resolution mass spectrometry metabolomics approach.
biomarkers
bovine
pathways
screening
steroids
Journal
Drug testing and analysis
ISSN: 1942-7611
Titre abrégé: Drug Test Anal
Pays: England
ID NLM: 101483449
Informations de publication
Date de publication:
May 2022
May 2022
Historique:
revised:
14
06
2021
received:
22
03
2021
accepted:
07
07
2021
pubmed:
10
7
2021
medline:
25
5
2022
entrez:
9
7
2021
Statut:
ppublish
Résumé
With the aim of specifically investigating patterns associated with three steroid treatments (17β-nandrolone, 17β-estradiol, and 17β-nandrolone + 17β-estradiol) in bovine, an reversed phase liquid chromatography (RPLC)-electrospray ionization (ESI)(+/-)-high-resolution mass spectrometry (HRMS) study was conducted to characterize the urinary profiles of involved animals. Although specific fingerprints with strong differences could be highlighted between urinary metabolite profiles within urine samples collected on control and treated animals, it appeared further that significant discriminations could also be observed between steroid treatments, evidencing thus specific patterns and candidate biomarkers associated to each treatment. An MS-2 structural elucidation step enabled level-1 identification of two biomarkers mainly involved in energy pathways, in relation to skeletal muscle functioning. These results make it possible to envisage a global strategy for the detection of anabolic practices involving steroids, while at the same time providing clues as to the compounds used, which would facilitate the confirmation stage to follow.
Substances chimiques
Anabolic Agents
0
Biomarkers
0
Steroids
0
Estradiol
4TI98Z838E
Nandrolone
6PG9VR430D
Types de publication
Journal Article
Langues
eng
Sous-ensembles de citation
IM
Pagination
879-886Informations de copyright
© 2021 John Wiley & Sons, Ltd.
Références
Council Directive 88/146/EEC. Prohibiting the use livestock farming of certain substances having a hormonal action. 1988 (16):16-18.
European Parliament and Council. REGULATION (EU) 2017/625 on official controls and other official activities performed to ensure the application of food and feed law, rules on animal health and welfare, plant health and plant protection products, amending Regulations (EC) No 999/2001, (EC) No 396/2005, (EC) No 1069/2009, (EC) No 1107/2009, (EU) No 1151/2012, (EU) No 652/2014, (EU) 2016/429 and (EU) 2016/2031 of the European Parliament and of the Council, Council Regulations (EC) No 1/2005 and (EC) No 1099/2009 and Council Directives 98/58/EC, 1999/74/EC, 2007/43/EC, 2008/119/EC and 2008/120/EC, and repealing Regulations (EC) No 854/2004 and (EC) No 882/2004 of the European Parliament and of the Council, Council Directives 89/608/EEC, 89/662/EEC, 90/425/EEC, 91/496/EEC, 96/23/EC, 96/93/EC and 97/78/EC and Council Decision 92/438/EEC (Official Controls Regulation). In. OJ L 95, 7, 4, 2017, pp. 1-142.
Council Directive 96/22/EC. Council Directive 96/22/EC of 29 April 1996 concerning the prohibition on the use in stockfarming of certain substances having a hormonal or thyrostatic action and of beta-agonists, and repealing Directives 81/602/EEC, 88/146/EEC and 88/299/EEC. 1996.
De Brabander HF, Le Bizec B, Pinel G, et al. Past, present and future of mass spectrometry in the analysis of residues of banned substances in meat-producing animals. J Mass Spectrom. 2007;42(8):983-998.
Dervilly-Pinel G, Rambaud L, Sitthisack P, et al. 5alpha-Estrane-3beta,17beta-diol and 5beta-estrane-3alpha,17beta-diol: definitive screening biomarkers to sign nandrolone abuse in cattle? J Steroid Biochem Mol Biol. 2011;126(3-5):65-71.
Destrez B, Bichon E, Rambaud L, et al. Criteria to distinguish between natural situations and illegal use of boldenone, boldenone esters and boldione in cattle 2. Direct measurement of 17beta-boldenone sulpho-conjugate in calf urine by liquid chromatography-high resolution and tandem mass spectrometry. Steroids. 2009;74(10-11):803-808.
Doue M, Dervilly-Pinel G, Pouponneau K, Monteau F, Le Bizec B. Analysis of glucuronide and sulfate steroids in urine by ultra-high-performance supercritical-fluid chromatography hyphenated tandem mass spectrometry. Anal Bioanal Chem. 2015;407(15):4473-4484.
Le Bizec B, Pinel G, Antignac JP. Options for veterinary drug analysis using mass spectrometry. J Chromatogr A. 2009;1216(46):8016-8034.
Pinel G, Rambaud L, Cacciatore G, et al. Elimination kinetic of 17beta-estradiol 3-benzoate and 17beta-nandrolone laureate ester metabolites in calves' urine. J Steroid Biochem Mol Biol. 2008;110(1-2):30-38.
Pinel G, Rambaud L, Monteau F, Elliot C, Le Bizec B. Estranediols profiling in calves' urine after 17 beta-nandrolone laureate ester administration. J Steroid Biochem. 2010;121(3-5):626-632.
Riedmaier I, Becker C, Pfaffl MW, Meyer HH. The use of omic technologies for biomarker development to trace functions of anabolic agents. J Chromatogr A. 2009;1216(46):8192-8199.
Riedmaier I, Benes V, Blake J, et al. RNA-sequencing as useful screening tool in the combat against the misuse of anabolic agents. Anal Chem. 2012;84(15):6863-6868.
Riedmaier I, Tichopad A, Reiter M, Pfaffl MW, Meyer HH. Influence of testosterone and a novel SARM on gene expression in whole blood of Macaca fascicularis. J Steroid Biochem Mol Biol. 2009;114(3-5):167-173.
Reiter M, Walf VM, Christians A, Pfaffl MW, Meyer HH. Modification of mRNA expression after treatment with anabolic agents and the usefulness for gene expression-biomarkers. Anal Chim Acta. 2007;586(1-2):73-81.
Nebbia C, Urbani A, Carletti M, et al. Novel strategies for tracing the exposure of meat cattle to illegal growth-promoters. Vet J. 2011;189(1):34-42.
Cacciatore G, Eisenberg SW, Situ C, et al. Effect of growth-promoting 17beta-estradiol, 19-nortestosterone and dexamethasone on circulating levels of nine potential biomarker candidates in veal calves. Anal Chim Acta. 2009;637(1-2):351-359.
Mooney MH, Bergwerff AA, van Meeuwen JA, Luppa PB, Elliott CT. Biosensor-based detection of reduced sex hormone-binding globulin binding capacities in response to growth-promoter administrations. Anal Chim Acta. 2009;637(1-2):235-240.
Mooney MH, Elliott CT, Le Bizec B. Combining biomarker screening and mass-spectrometric analysis to detect hormone abuse in cattle. TrAC Trends Anal Chem. 2009;28(6):665-675.
Courant F, Pinel G, Bichon E, Monteau F, Antignac JP, Le Bizec B. Development of a metabolomic approach based on liquid chromatography-high resolution mass spectrometry to screen for clenbuterol abuse in calves. Analyst. 2009;134(8):1637-1646.
Dervilly-Pinel G, Chereau S, Cesbron N, Monteau F, Le Bizec B. LC-HRMS based metabolomics screening model to detect various β-agonists treatments in bovines. Metabolomics. 2015;11(2):403-411.
Dervilly-Pinel G, Courant F, Chereau S, et al. Metabolomics in food analysis: application to the control of forbidden substances. Drug Test Anal. 2012;4:59-69.
Dervilly-Pinel G, Royer AL, Bozzetta E, et al. When LC-HRMS metabolomics gets ISO17025 accredited and ready for official controls-application to the screening of forbidden compounds in livestock. Food Addit Contam Part A-Chem Anal Control Expos Risk Assess. 2018;35(10):1948-1958.
Dervilly-Pinel G, Weigel S, Lommen A, et al. Assessment of two complementary liquid chromatography coupled to high resolution mass spectrometry metabolomics strategies for the screening of anabolic steroid treatment in calves. Anal Chim Acta. 2011;700(1-2):144-154.
Gallart Ayala H, Chéreau S, Dervilly-Pinel G, Le Bizec B. Potential of mass spectrometry metabolomics for chemical food safety. Bioanal Rev. 2015;7(1):133-146.
Guitton Y, Dervilly-Pinel G, Jandova R, Stead S, Takats Z, Le Bizec B. Rapid evaporative ionisation mass spectrometry and chemometrics for high-throughput screening of growth promoters in meat producing animals. Food Addit Contam Part A-Chem Anal Control Expos Risk Assess. 2018;35(5):900-910.
Jacob C, Dervilly-Pinel G, Biancotto G, Monteau F, Le Bizec B. Global urine fingerprinting by LC-ESI(+)-HRMS for better characterization of metabolic pathway disruption upon anabolic practices in bovine. Metabolomics. 2014;11:184-197.
Kieken F, Pinel G, Antignac J, et al. Generation and processing of urinary and plasmatic metabolomic fingerprints to reveal an illegal administration of recombinant equine growth hormone from LC-HRMS measurements. Metabolomics. 2011;7:84-93.
Kieken F, Pinel G, Antignac JP, et al. Development of a metabonomic approach based on LC-ESI-HRMS measurements for profiling of metabolic changes induced by recombinant equine growth hormone in horse urine. Anal Bioanal Chem. 2009;394(8):2119-2128.
Kouassi Nzoughet J, Dervilly-Pinel G, Chereau S, et al. First insights into serum metabolomics of trenbolone/estradiol implanted bovines: screening model to predict hormone-treated and control animals' status. Metabolomics. 2015;11(5):1184-1196.
Kouassi Nzoughet J, Gallart-Ayala H, Dervilly-Pinel G, Biancotto G, Le Bizec B. Original combination of hydrophilic interaction (HILIC) and reverse phase (RPLC) high resolution LC-MS for characterizing lipids profile disruption in serum of anabolic implanted bovines. Metabolomics. 2015;11(6):1884-1895.
Marchand J, Martineau E, Guitton Y, Le Bizec B, Dervilly-Pinel G, Giraudeau P. A multidimensional H-1 NMR lipidomics workflow to address chemical food safety issues. Metabolomics. 2018;14(5):60.
Peng T, Royer AL, Guitton Y, Le Bizec B, Dervilly-Pinel G. Serum-based metabolomics characterization of pigs treated with ractopamine. Metabolomics. 2017;13(6):1-15.
Pinel G, Weigel S, Antignac JP, et al. Targeted and untargeted profiling of biological fluids to screen for anabolic practices in cattle. TrAC Trends Anal Chem. 2010;29(11):1269-1280.
Esslinger S, Riedl J, Fauhl-Hassek C. Potential and limitations of non-targeted fingerprinting for authentication of food in official control. Food Res Int. 2014;60:189-204.
Riedl J, Esslinger S, Fauhl-Hassek C. Review of validation and reporting of non-targeted fingerprinting approaches for food authentication. Anal Chim Acta. 2015;885:17-32.
Pinel G, Weigel S, Lommen A, et al. Assessment of two complementary LC-HRMS metabolomics strategies for the screening of anabolic steroid treatment in calves. Anal Chim Acta. 2011;700(1-2):144-154.
Giacomoni F, Le Corguille G, Monsoor M, et al. Workflow4Metabolomics: a collaborative research infrastructure for computational metabolomics. Bioinformatics. 2015;31(9):1493-1495.
Smith CA, Want EJ, O'Maille G, Abagyan R, Siuzdak G. XCMS: processing mass spectrometry data for metabolite profiling using nonlinear peak alignment, matching and identification. Anal Chem. 2006;78(3):779.
Liquet B, Cao K, Hocini H, Thiébaut R. A novel approach for biomarker selection and the integration of repeated measures experiments from two assays. BMC Bioinf. 2012;13(325):1-14.
Westerhuis J, van Velzen E, Hoefsloot H, Smilde A. Multivariate paired data analysis: multilevel PLSDA versus OPLSDA. Metabolomics. 2010;6(1):119-128.
Guitton Y, Tremblay-Franco M, Le Corguille G, et al. Create, run, share, publish, and reference your LC-MS, FIA-MS, GC-MS, and NMR data analysis workflows with the Workflow4Metabolomics 3.0 Galaxy online infrastructure for metabolomics. Int J Biochem Cell Biol. 2017;93:89-101.
Schymansky E, Jeon J, Gulde R, et al. Identifying small molecules via high resolution mass spectrometry: communicating confidence. Environ Sci Technol. 2014;48(4):2097.
van der Kloet FM, Bobeldijk I, Verheij ER, Jellema RH. Analytical error reduction using single point calibration for accurate and precise metabolomic phenotyping. J Proteome Res. 2009;8(11):5132-5141.
Chaleckis R, Meister I, Zhang P, Wheelock C. Challenges, progress and promises of metabolite annotation for LC-MS-based metabolomics. Curr Opin Biotechnol. 2019;55:44-50.
(KEGG). KEoGaG. Arginine and Proline metabolism. Creatine pathway. https://www.genome.jp/pathway/map 00330+M00047. Accessed 11th June, 2021.
Spriet LL, Whitfield J. Taurine and skeletal muscle function. Curr Opin Clin Nutr Metab Care. 2015;18(1):96-101.