MRSA strains with distinct accessory genes predominate at different ages in cystic fibrosis.
MRSA
Staphylococcus aureus
birth cohort effect
cystic fibrosis
whole genome sequencing
Journal
Pediatric pulmonology
ISSN: 1099-0496
Titre abrégé: Pediatr Pulmonol
Pays: United States
ID NLM: 8510590
Informations de publication
Date de publication:
09 2021
09 2021
Historique:
revised:
28
06
2021
received:
26
01
2021
accepted:
29
06
2021
pubmed:
6
7
2021
medline:
25
11
2021
entrez:
5
7
2021
Statut:
ppublish
Résumé
Methicillin resistant Staphylococcus aureus (MRSA) is prevalent and consequential in cystic fibrosis (CF). Whole genome sequencing (WGS) could reveal genomic differences in MRSA associated with poorer outcomes or detect MRSA transmission. To identify MRSA genes associated with low lung function and potential MRSA transmission in CF. We collected 97 MRSA isolates from 74 individuals with CF from 2017 and performed short-read WGS. We determined sequence type (ST) and the phylogenetic relationship between isolates. We aligned accessory genes from 25 reference genomes to genome assemblies, classified isolates by accessory gene content, and correlated the accessory genome to clinical outcomes. The most prevalent ST were ST5 (N = 55), ST8 (N = 15), and ST105 (N = 14). Closely related MRSA strains were shared by family members with CF, but rarely between unrelated individuals. Three clusters of MRSA were identified by accessory genome content. Cluster A, including ST5 and ST105, was highly prevalent at all ages. Cluster B, including ST8, was more limited to younger patients. Cluster C included 6 distantly related strains. Patients 20 years old and younger infected with Cluster A had lower forced expiratory volume in the first second (FEV In this CF cohort, we identified MRSA subtypes that predominate at different ages and differ by accessory gene content. The most prevalent cluster of MRSA, including ST5 and ST105, was associated with lower FEV
Identifiants
pubmed: 34219414
doi: 10.1002/ppul.25559
pmc: PMC8395597
mid: NIHMS1721372
doi:
Types de publication
Journal Article
Research Support, N.I.H., Extramural
Research Support, Non-U.S. Gov't
Langues
eng
Sous-ensembles de citation
IM
Pagination
2868-2878Subventions
Organisme : NCATS NIH HHS
ID : UL1 TR002537
Pays : United States
Organisme : Intramural NIH HHS
ID : Z99 CL999999
Pays : United States
Organisme : NIDDK NIH HHS
ID : P30 DK054759
Pays : United States
Organisme : NHLBI NIH HHS
ID : K08 HL136927
Pays : United States
Organisme : NIAID NIH HHS
ID : T32 AI007343
Pays : United States
Informations de copyright
© 2021 Wiley Periodicals LLC.
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