Altered Induction of Reactive Oxygen Species by X-rays in Hematopoietic Cells of C57BL/6-Tg (CAG-EGFP) Mice.
GFP transgenic mice
green fluorescent protein (GFP)
hematopoietic cells
ionizing radiation
reactive oxygen species (ROS)
Journal
International journal of molecular sciences
ISSN: 1422-0067
Titre abrégé: Int J Mol Sci
Pays: Switzerland
ID NLM: 101092791
Informations de publication
Date de publication:
28 Jun 2021
28 Jun 2021
Historique:
received:
24
05
2021
revised:
14
06
2021
accepted:
15
06
2021
entrez:
2
7
2021
pubmed:
3
7
2021
medline:
10
8
2021
Statut:
epublish
Résumé
Previous work pointed to a critical role of excessive production of reactive oxygen species (ROS) in increased radiation hematopoietic death in GFP mice. Meanwhile, enhanced antioxidant capability was not demonstrated in the mouse model of radio-induced adaptive response (RAR) using rescue of radiation hematopoietic death as the endpoint. ROS induction by ex vivo X-irradiation at a dose ranging from 0.1 to 7.5 Gy in the nucleated bone marrow cells was comparatively studied using GFP and wild type (WT) mice. ROS induction was also investigated in the cells collected from mice receiving a priming dose (0.5 Gy) efficient for RAR induction in WT mice. Significantly elevated background and increased induction of ROS in the cells from GFP mice were observed compared to those from WT mice. Markedly lower background and decreased induction of ROS were observed in the cells collected from WT mice but not GFP mice, both receiving the priming dose. GFP overexpression could alter background and induction of ROS by X-irradiation in hematopoietic cells. The results provide a reasonable explanation to the previous study on the fate of cells and mice after X-irradiation and confirm enhanced antioxidant capability in RAR. Investigations involving GFP overexpression should be carefully interpreted.
Identifiants
pubmed: 34203224
pii: ijms22136929
doi: 10.3390/ijms22136929
pmc: PMC8268547
pii:
doi:
Substances chimiques
Reactive Oxygen Species
0
Types de publication
Journal Article
Langues
eng
Sous-ensembles de citation
IM
Subventions
Organisme : JSPS KAKENHI
ID : JSPS KAKENHI 20K08065
Organisme : JSPS KAKENHI
ID : JSPS KAKENHI 25340041
Références
Anal Bioanal Chem. 2017 Jul;409(18):4377-4381
pubmed: 28527000
Hum Exp Toxicol. 2015 Mar;34(3):272-83
pubmed: 24925363
Exp Cell Res. 2014 Jan 1;320(1):33-45
pubmed: 23899627
Biomed Pharmacother. 2018 Oct;106:1126-1134
pubmed: 30119179
Phys Med. 2001;17 Suppl 1:210-4
pubmed: 11776264
Blood. 2001 Apr 1;97(7):1951-9
pubmed: 11264158
Radiat Res. 2015 Apr;183(4):455-64
pubmed: 25807320
Biochem Biophys Res Commun. 1996 Oct 23;227(3):707-11
pubmed: 8885998
Genes Cells. 2018 Jul;23(7):580-589
pubmed: 29749672
Genomics. 2002 Dec;80(6):564-74
pubmed: 12504848
Cell Death Differ. 2017 Feb;24(2):288-299
pubmed: 27911443
Gene Ther. 1999 Jul;6(7):1305-12
pubmed: 10455440
Mech Dev. 1998 Aug;76(1-2):79-90
pubmed: 9867352
J Fish Biol. 2018 Jan;92(1):177-189
pubmed: 29194605
J Mol Cell Cardiol. 2008 Feb;44(2):405-10
pubmed: 18048055
Commun Biol. 2020 May 29;3(1):273
pubmed: 32472011
J Radiat Res. 2007 Mar;48(2):121-8
pubmed: 17339749
Proc Natl Acad Sci U S A. 1998 Oct 27;95(22):13042-7
pubmed: 9789037
Pediatr Res. 2009 Aug;66(2):121-7
pubmed: 19390491
Nat Methods. 2005 Dec;2(12):905-9
pubmed: 16299475
J Proteome Res. 2013 Aug 2;12(8):3801-8
pubmed: 23827011
Radiat Res. 2005 Oct;164(4 Pt 2):527-30
pubmed: 16187762
Exp Mol Med. 2020 Feb;52(2):192-203
pubmed: 32060354
Mol Ther Nucleic Acids. 2013 Apr 16;2:e86
pubmed: 23591809
Redox Biol. 2017 Aug;12:462-468
pubmed: 28334681
Nat Med. 2000 May;6(5):483-4
pubmed: 10802678
Stem Cell Rev Rep. 2016 Oct;12(5):553-559
pubmed: 27435468
Kaohsiung J Med Sci. 2020 Aug;36(8):630-639
pubmed: 32363780
React Oxyg Species (Apex). 2016;1(1):9-21
pubmed: 29707643
Oxid Med Cell Longev. 2016;2016:7524308
pubmed: 27703600
Oxid Med Cell Longev. 2019 Nov 12;2019:3010342
pubmed: 31781332
Clin Cancer Res. 2020 Nov 15;26(22):5781-5790
pubmed: 32554542
Nat Chem Biol. 2015 Jan;11(1):64-70
pubmed: 25402766
Physiol Res. 2019 Aug 29;68(4):525-530
pubmed: 31342754
Nat Rev Mol Cell Biol. 2002 Dec;3(12):906-18
pubmed: 12461557
Radiat Prot Dosimetry. 2006;122(1-4):301-6
pubmed: 17166874
BMC Biotechnol. 2002 Jun 11;2:11
pubmed: 12079497
Gene. 1996;173(1 Spec No):83-7
pubmed: 8707061
Physiol Rev. 2014 Jul;94(3):909-50
pubmed: 24987008
J Cell Biochem. 2010 Aug 15;110(6):1439-46
pubmed: 20506255
Int J Mol Sci. 2019 May 15;20(10):
pubmed: 31096608
Mol Cancer Ther. 2003 Sep;2(9):911-7
pubmed: 14555710
J Biosci. 2016 Jun;41(2):193-203
pubmed: 27240980
Gene Ther. 2000 Dec;7(23):2036-40
pubmed: 11175316
Free Radic Biol Med. 2018 Sep;125:62-71
pubmed: 29550327
Int J Radiat Biol. 2019 Aug;95(8):1144-1149
pubmed: 30822211
Dose Response. 2020 Aug 26;18(3):1559325820951332
pubmed: 32922229
Gene. 2012 Dec 10;511(1):1-6
pubmed: 22981713
J Biosci. 2018 Jul;43(3):421-430
pubmed: 30002261
Free Radic Biol Med. 2015 Nov;88(Pt B):168-178
pubmed: 26119783
Cells. 2019 Sep 18;8(9):
pubmed: 31540530
Biochem Biophys Res Commun. 1999 Jul 14;260(3):712-7
pubmed: 10403831
Radiat Environ Biophys. 2007 Mar;46(1):1-12
pubmed: 17131131
Redox Biol. 2017 Aug;12:755-757
pubmed: 28415040
Exp Hematol. 2004 Feb;32(2):210-7
pubmed: 15102483
Gene Ther. 2008 May;15(9):700-1
pubmed: 18288211
Redox Biol. 2017 Apr;11:613-619
pubmed: 28110218
Front Public Health. 2020 Dec 15;8:601711
pubmed: 33384980
Biochim Biophys Acta. 2016 Dec;1863(12):2977-2992
pubmed: 27646922
Annu Rev Biochem. 1998;67:509-44
pubmed: 9759496
Nat Med. 2000 May;6(5):482-3
pubmed: 10802676
Luminescence. 2003 Jan-Feb;18(1):1-18
pubmed: 12536374
J Fluoresc. 2013 Jul;23(4):613-9
pubmed: 23475276
Braz J Microbiol. 2019 Apr;50(2):347-355
pubmed: 30877662
Redox Biol. 2015 Aug;5:275-289
pubmed: 26079210
Genome Res. 2013 Feb;23(2):300-11
pubmed: 23275495
Auto Immun Highlights. 2016 Dec;7(1):11
pubmed: 27491295
J Am Chem Soc. 2006 Apr 12;128(14):4766-72
pubmed: 16594713
Cell Stress Chaperones. 2016 Mar;21(2):367-72
pubmed: 26634370
FEBS Lett. 1997 May 5;407(3):313-9
pubmed: 9175875
Cell Syst. 2019 Feb 27;8(2):163-167.e2
pubmed: 30797774
Biochem Biophys Res Commun. 1997 Jul 18;236(2):347-50
pubmed: 9240438
Chem Biol Interact. 2014 Dec 5;224:164-75
pubmed: 25452175
Biotechnol Bioeng. 2005 Mar 5;89(5):574-87
pubmed: 15672380
Indian J Clin Biochem. 2015 Jan;30(1):11-26
pubmed: 25646037
Nat Med. 2000 Mar;6(3):271-7
pubmed: 10700228
Nat Med. 1996 Feb;2(2):216-23
pubmed: 8574968
Int J Radiat Biol. 2020 May;96(5):561-576
pubmed: 31976798
Proc Natl Acad Sci U S A. 1994 Dec 20;91(26):12501-4
pubmed: 7809066
J Thromb Haemost. 2013 Sep;11(9):1716-26
pubmed: 23865569