Detection of porcine circovirus type 2 (PCV2) in the Philippines and the complexity of PCV2-associated disease diagnosis.
Histopathology
Immunohistochemistry
PCVAD diagnosis
Porcine circovirus type 2
qPCR
Journal
Tropical animal health and production
ISSN: 1573-7438
Titre abrégé: Trop Anim Health Prod
Pays: United States
ID NLM: 1277355
Informations de publication
Date de publication:
25 Jun 2021
25 Jun 2021
Historique:
received:
07
02
2021
accepted:
18
06
2021
entrez:
26
6
2021
pubmed:
27
6
2021
medline:
30
6
2021
Statut:
epublish
Résumé
Porcine circovirus type 2 (PCV2), an important pig viral pathogen, can cause porcine circovirus-associated disease (PCVAD), resulting in economic losses associated with decreased growth and mortalities. The diagnosis of PCVAD is complex requiring clinical, pathological and virological approaches. This study assessed PCV2 infection using histopathology and immunohistochemistry (IHC) on tissue samples and quantitative polymerase chain reaction (qPCR) on serum samples from 47 grower-finisher pigs allocated in three clinical groups in the Philippines. Typical PCV2 histopathological lesions were observed in mediastinal lymph nodes (MLN) of eight of 47 pigs. Lymphoid depletion was seen in all eight pigs and granulomatous inflammation in one of these pigs. Four of these eight pigs were PCV2 positive by both IHC and qPCR. IHC revealed PCV2 antigen in 8 pigs in at least one of the following tissues: MLN (5/8), spleen (3/8), tonsils (4/8) and lungs (5/8). PCV2 antigen was observed in 3/8 MLN with lymphoid depletion and in one MLN with depletion and granulomatous inflammation. The qPCR test showed that 33 sera had a non-detectable level, twelve had < 10
Identifiants
pubmed: 34173084
doi: 10.1007/s11250-021-02823-y
pii: 10.1007/s11250-021-02823-y
doi:
Substances chimiques
Antibodies, Viral
0
Types de publication
Journal Article
Langues
eng
Sous-ensembles de citation
IM
Pagination
371Subventions
Organisme : Australian Centre for International Agricultural Research
ID : AH/2009/022
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