Stalling chromophore synthesis of the fluorescent protein Venus reveals the molecular basis of the final oxidation step.
Journal
Chemical science
ISSN: 2041-6520
Titre abrégé: Chem Sci
Pays: England
ID NLM: 101545951
Informations de publication
Date de publication:
31 Mar 2021
31 Mar 2021
Historique:
entrez:
25
6
2021
pubmed:
26
6
2021
medline:
26
6
2021
Statut:
epublish
Résumé
Fluorescent proteins (FPs) have revolutionised the life sciences, but the mechanism of chromophore maturation is still not fully understood. Here we show that incorporation of a photo-responsive non-canonical amino acid within the chromophore stalls maturation of Venus, a yellow FP, at an intermediate stage; a crystal structure indicates the presence of O
Identifiants
pubmed: 34168826
doi: 10.1039/d0sc06693a
pii: d0sc06693a
pmc: PMC8188506
doi:
Types de publication
Journal Article
Langues
eng
Pagination
7735-7745Subventions
Organisme : Biotechnology and Biological Sciences Research Council
ID : BB/H003746/1
Pays : United Kingdom
Informations de copyright
This journal is © The Royal Society of Chemistry.
Déclaration de conflit d'intérêts
There are no conflicts to declare.
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