Analysis of the light regulatory mechanism in carotenoid production in Rhodosporidium toruloides NBRC 10032.
Rhodosporidium toruloides
carotenogenesis
light response
Journal
Bioscience, biotechnology, and biochemistry
ISSN: 1347-6947
Titre abrégé: Biosci Biotechnol Biochem
Pays: England
ID NLM: 9205717
Informations de publication
Date de publication:
23 Jul 2021
23 Jul 2021
Historique:
received:
28
04
2021
accepted:
06
06
2021
pubmed:
15
6
2021
medline:
28
10
2021
entrez:
14
6
2021
Statut:
ppublish
Résumé
Light stimulates carotenoid production in an oleaginous yeast Rhodosporidium toruloides NBRC 10032 by promoting carotenoid biosynthesis genes. These genes undergo two-step transcriptional activation. The potential light regulator, Cryptochrome DASH (CRY1), has been suggested to contribute to this mechanism. In this study, based on KU70 (a component of nonhomologous end joining (NHEJ)) disrupting background, CRY1 disruptant was constructed to clarify CRY1 function. From analysis of CRY1 disruptant, it was suggested that CRY1 has the activation role of the carotenogenic gene expression. To obtain further insights into the light response, mutants varying carotenoid production were generated. Through analysis of mutants, the existence of the control two-step gene activation was proposed. In addition, our data analysis showed the strong possibility that R. toruloides NBRC 10032 is a homo-diploid strain.
Identifiants
pubmed: 34124766
pii: 6298221
doi: 10.1093/bbb/zbab109
doi:
Substances chimiques
Cryptochromes
0
Carotenoids
36-88-4
Types de publication
Journal Article
Langues
eng
Sous-ensembles de citation
IM
Pagination
1899-1909Subventions
Organisme : New Energy and Industrial Technology Development Organization
ID : P16009
Organisme : Science and Technology Research Promotion Program for Agriculture, Forestry, Fisheries and Food Industry
Informations de copyright
© The Author(s) 2021. Published by Oxford University Press on behalf of Japan Society for Bioscience, Biotechnology, and Agrochemistry.