Loss of the Bacterial Flagellar Motor Switch Complex upon Cell Lysis.
cell lysis
cryo-ET
flagellar motor
switch complex
Journal
mBio
ISSN: 2150-7511
Titre abrégé: mBio
Pays: United States
ID NLM: 101519231
Informations de publication
Date de publication:
29 06 2021
29 06 2021
Historique:
pubmed:
9
6
2021
medline:
16
11
2021
entrez:
8
6
2021
Statut:
ppublish
Résumé
The bacterial flagellar motor is a complex macromolecular machine whose function and self-assembly present a fascinating puzzle for structural biologists. Here, we report that in diverse bacterial species, cell lysis leads to loss of the cytoplasmic switch complex and associated ATPase before other components of the motor. This loss may be prevented by the formation of a cytoplasmic vesicle around the complex. These observations suggest a relatively loose association of the switch complex with the rest of the flagellar machinery.
Identifiants
pubmed: 34098733
doi: 10.1128/mBio.00298-21
pmc: PMC8263016
doi:
Substances chimiques
Bacterial Proteins
0
Types de publication
Journal Article
Research Support, N.I.H., Extramural
Research Support, Non-U.S. Gov't
Langues
eng
Sous-ensembles de citation
IM
Pagination
e0029821Subventions
Organisme : NIAID NIH HHS
ID : R01 AI127401
Pays : United States
Références
J Bacteriol. 2014 Aug;196(15):2775-88
pubmed: 24837291
Elife. 2019 Jan 16;8:
pubmed: 30648971
J Mol Biol. 2010 Feb 12;396(1):105-17
pubmed: 19925807
J Struct Biol. 2015 Nov;192(2):279-86
pubmed: 26087141
EMBO J. 1996 May 15;15(10):2393-406
pubmed: 8665847
J Mol Biol. 2006 Dec 15;364(5):921-37
pubmed: 17052729
J Bacteriol. 1971 Jan;105(1):376-83
pubmed: 4993324
EMBO J. 2019 Jul 15;38(14):e100957
pubmed: 31304634
J Mol Biol. 2021 Jun 25;433(13):167004
pubmed: 33891903
Trends Microbiol. 2020 Oct;28(10):785-788
pubmed: 32456977
J Bacteriol. 2019 Jun 10;201(13):
pubmed: 31010901
J Bacteriol. 1985 Mar;161(3):836-49
pubmed: 2982790
Proc Natl Acad Sci U S A. 2018 Dec 26;115(52):13365-13370
pubmed: 30541885
Proc Natl Acad Sci U S A. 2020 Apr 21;117(16):8941-8947
pubmed: 32241888
J Bacteriol. 1977 Feb;129(2):678-89
pubmed: 402356
Annu Rev Microbiol. 2003;57:77-100
pubmed: 12730325
J Mol Biol. 1994 Jan 28;235(4):1261-70
pubmed: 8308888
Proc Natl Acad Sci U S A. 2003 Mar 18;100(6):3027-30
pubmed: 12631703
Microbiologyopen. 2014 Oct;3(5):702-10
pubmed: 25065852
Proc Natl Acad Sci U S A. 2009 Oct 6;106(40):17181-6
pubmed: 19805102
J Bacteriol. 1992 Jan;174(1):263-8
pubmed: 1370283
PLoS Biol. 2019 Mar 19;17(3):e3000165
pubmed: 30889173
Nature. 2006 Aug 31;442(7106):1062-4
pubmed: 16885937
Biomolecules. 2020 Nov 09;10(11):
pubmed: 33182435
Cell. 2021 May 13;184(10):2665-2679.e19
pubmed: 33882274
PLoS Genet. 2012 Sep;8(9):e1002983
pubmed: 23028376
Proc Natl Acad Sci U S A. 2007 Apr 24;104(17):7116-21
pubmed: 17438286
Proc Natl Acad Sci U S A. 1992 Jul 1;89(13):5956-60
pubmed: 1631080
Proc Natl Acad Sci U S A. 1982 Mar;79(6):1830-3
pubmed: 7043465
EMBO J. 2011 Jun 14;30(14):2972-81
pubmed: 21673657
J Bacteriol. 2013 Sep;195(17):3940-6
pubmed: 23813732
Philos Trans R Soc Lond B Biol Sci. 2015 Oct 5;370(1679):
pubmed: 26370933
Mol Microbiol. 2020 Aug;114(2):279-291
pubmed: 32259388
J Bacteriol. 2010 Jan;192(1):375-8
pubmed: 19880601
J Mol Biol. 1990 Feb 20;211(4):669-72
pubmed: 2179562