Loss of the Bacterial Flagellar Motor Switch Complex upon Cell Lysis.


Journal

mBio
ISSN: 2150-7511
Titre abrégé: mBio
Pays: United States
ID NLM: 101519231

Informations de publication

Date de publication:
29 06 2021
Historique:
pubmed: 9 6 2021
medline: 16 11 2021
entrez: 8 6 2021
Statut: ppublish

Résumé

The bacterial flagellar motor is a complex macromolecular machine whose function and self-assembly present a fascinating puzzle for structural biologists. Here, we report that in diverse bacterial species, cell lysis leads to loss of the cytoplasmic switch complex and associated ATPase before other components of the motor. This loss may be prevented by the formation of a cytoplasmic vesicle around the complex. These observations suggest a relatively loose association of the switch complex with the rest of the flagellar machinery.

Identifiants

pubmed: 34098733
doi: 10.1128/mBio.00298-21
pmc: PMC8263016
doi:

Substances chimiques

Bacterial Proteins 0

Types de publication

Journal Article Research Support, N.I.H., Extramural Research Support, Non-U.S. Gov't

Langues

eng

Sous-ensembles de citation

IM

Pagination

e0029821

Subventions

Organisme : NIAID NIH HHS
ID : R01 AI127401
Pays : United States

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Auteurs

Mohammed Kaplan (M)

Division of Biology and Biological Engineering, California Institute of Technology, Pasadena, California, USA.

Elitza I Tocheva (EI)

Division of Biology and Biological Engineering, California Institute of Technology, Pasadena, California, USA.

Ariane Briegel (A)

Division of Biology and Biological Engineering, California Institute of Technology, Pasadena, California, USA.

Megan J Dobro (MJ)

Division of Biology and Biological Engineering, California Institute of Technology, Pasadena, California, USA.

Yi-Wei Chang (YW)

Division of Biology and Biological Engineering, California Institute of Technology, Pasadena, California, USA.

Poorna Subramanian (P)

Division of Biology and Biological Engineering, California Institute of Technology, Pasadena, California, USA.

Alasdair W McDowall (AW)

Division of Biology and Biological Engineering, California Institute of Technology, Pasadena, California, USA.

Morgan Beeby (M)

Division of Biology and Biological Engineering, California Institute of Technology, Pasadena, California, USA.

Grant J Jensen (GJ)

Division of Biology and Biological Engineering, California Institute of Technology, Pasadena, California, USA.
Department of Chemistry and Biochemistry, Brigham Young University, Provo, Utah, USA.

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Classifications MeSH