Standardised pomegranate peel extract lavage prevents postoperative peritoneal adhesion by regulating TGF-β and VEGF levels.
Animals
Fruit
Male
Peritoneal Lavage
/ methods
Plant Extracts
/ administration & dosage
Pomegranate
Postoperative Complications
/ metabolism
Rats
Rats, Wistar
Tissue Adhesions
/ metabolism
Transforming Growth Factor beta
/ antagonists & inhibitors
Vascular Endothelial Growth Factor A
/ antagonists & inhibitors
Anti-inflammatory
Antioxidant
Peritoneal adhesion
Pomegranate
Punica granatum
Journal
Inflammopharmacology
ISSN: 1568-5608
Titre abrégé: Inflammopharmacology
Pays: Switzerland
ID NLM: 9112626
Informations de publication
Date de publication:
Jun 2021
Jun 2021
Historique:
received:
05
11
2020
accepted:
04
05
2021
pubmed:
17
5
2021
medline:
15
12
2021
entrez:
16
5
2021
Statut:
ppublish
Résumé
Peritoneal adhesion represents a severe complication following surgery. Punica granatum (pomegranate) possesses several anti-oxidative and anti-inflammatory properties. Pomegranate peel extract (PPEx) can alleviate the production of various inflammatory factors and cytokines. Thus, we sought to evaluate the anti-adhesion effects of pomegranate in rats. Thirty male Wistar rats (6-week-old, 220 ± 20 g) were divided into five groups (n = 6): normal group without any surgical procedures, control group, and experimental groups receiving 2 ml of 1%, 2%, and 4% w/v PPEx, respectively. Peritoneal adhesions were examined macroscopically. Furthermore, we evaluated inflammatory cytokines levels [interleukin 6 (IL-6), and tumour necrosis factor-α (TNF-α)], growth factors [transforming growth factor- β1 (TGF-β1), and vascular endothelial growth factor (VEGF)], and oxidative stress parameters [nitric oxide metabolites (NO), and malondialdehyde (MDA), and glutathione (GSH)] using biochemical methods. Our results showed that the adhesion score and IL-6, TNF-α, TGF-β1, VEGF, NO, and MDA levels were increased in the control group. In contrast, the GSH level was diminished in the control group compared with the normal group (P < 0.001). PPEx (1 and 2% w/v) markedly reduced all measured parameters compared with the control group (P < 0.001-0.05). PPEx may reduce peritoneal adhesion by alleviating adhesion formation, IL-6, TNF-α, TGF-β1, VEGF, NO, and MDA, and stimulating anti-oxidative factors. Therefore, PPEx may be considered an appropriate candidate for the treatment of postoperative peritoneal adhesion.
Identifiants
pubmed: 33993390
doi: 10.1007/s10787-021-00819-6
pii: 10.1007/s10787-021-00819-6
doi:
Substances chimiques
Plant Extracts
0
Transforming Growth Factor beta
0
Vascular Endothelial Growth Factor A
0
vascular endothelial growth factor A, rat
0
Types de publication
Journal Article
Langues
eng
Sous-ensembles de citation
IM
Pagination
855-868Subventions
Organisme : Mashhad University of Medical Sciences
ID : 961726
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