Crystal and solution structures reveal oligomerization of individual capsid homology domains of Drosophila Arc.
Journal
PloS one
ISSN: 1932-6203
Titre abrégé: PLoS One
Pays: United States
ID NLM: 101285081
Informations de publication
Date de publication:
2021
2021
Historique:
received:
24
11
2020
accepted:
23
03
2021
entrez:
14
5
2021
pubmed:
15
5
2021
medline:
26
10
2021
Statut:
epublish
Résumé
Synaptic plasticity is vital for brain function and memory formation. One of the key proteins in long-term synaptic plasticity and memory is the activity-regulated cytoskeleton-associated protein (Arc). Mammalian Arc forms virus-like capsid structures in a process requiring the N-terminal domain and contains two C-terminal lobes that are structural homologues to retroviral capsids. Drosophila has two isoforms of Arc, dArc1 and dArc2, with low sequence similarity to mammalian Arc, but lacking a large N-terminal domain. Both dArc isoforms are related to the Ty3/gypsy retrotransposon capsid, consisting of N- and C-terminal lobes. Structures of dArc1, as well as capsids formed by both dArc isoforms, have been recently determined. We carried out structural characterization of the four individual dArc lobe domains. As opposed to the corresponding mammalian Arc lobe domains, which are monomeric, the dArc lobes were all oligomeric in solution, indicating a strong propensity for homophilic interactions. A truncated N-lobe from dArc2 formed a domain-swapped dimer in the crystal structure, resulting in a novel dimer interaction that could be relevant for capsid assembly or other dArc functions. This domain-swapped structure resembles the dimeric protein C of flavivirus capsids, as well as the structure of histones dimers, domain-swapped transcription factors, and membrane-interacting BAK domains. The strong oligomerization properties of the isolated dArc lobe domains explain the ability of dArc to form capsids in the absence of any large N-terminal domain, in contrast to the mammalian protein.
Identifiants
pubmed: 33989344
doi: 10.1371/journal.pone.0251459
pii: PONE-D-20-36347
pmc: PMC8121366
doi:
Substances chimiques
Cytoskeletal Proteins
0
Nerve Tissue Proteins
0
activity regulated cytoskeletal-associated protein
0
Types de publication
Journal Article
Research Support, Non-U.S. Gov't
Langues
eng
Sous-ensembles de citation
IM
Pagination
e0251459Déclaration de conflit d'intérêts
The authors have declared that no competing interests exist.
Références
Protein Cell. 2015 Sep;6(9):693-7
pubmed: 26186914
J Mol Biol. 2007 Sep 21;372(3):774-97
pubmed: 17681537
Nat Neurosci. 2013 Jul;16(7):874-83
pubmed: 23749147
Protein Sci. 2018 Jan;27(1):293-315
pubmed: 29067766
Acta Crystallogr D Biol Crystallogr. 1999 Jan;55(Pt 1):85-92
pubmed: 10089398
Cell. 2013 Jan 31;152(3):519-31
pubmed: 23374347
Nucleic Acids Res. 2010 Jul;38(Web Server issue):W545-9
pubmed: 20457744
J Biotechnol. 2006 Feb 10;121(3):291-8
pubmed: 16150509
Curr Opin Microbiol. 2006 Oct;9(5):520-5
pubmed: 16920388
Nucleic Acids Res. 2009 Jul;37(Web Server issue):W480-4
pubmed: 19465380
Proc Natl Acad Sci U S A. 1989 Jul;86(13):5015-9
pubmed: 2544887
Structure. 2019 Jul 2;27(7):1071-1081.e5
pubmed: 31080121
J Appl Crystallogr. 2015 Mar 12;48(Pt 2):431-443
pubmed: 25844078
J Comput Chem. 2004 Oct;25(13):1605-12
pubmed: 15264254
Mol Biol Evol. 2013 Apr;30(4):772-80
pubmed: 23329690
J Appl Crystallogr. 2017 Jun 26;50(Pt 4):1212-1225
pubmed: 28808438
Sci Rep. 2015 Nov 13;5:16520
pubmed: 26563764
Proc Natl Acad Sci U S A. 2010 Oct 19;107(42):18173-8
pubmed: 20921410
J Biol Chem. 2002 Jul 26;277(30):27103-8
pubmed: 12019266
Structure. 2009 May 13;17(5):737-48
pubmed: 19446529
Biochem Biophys Rep. 2021 Mar 05;26:100975
pubmed: 33732907
J Neurochem. 2018 Nov;147(3):323-343
pubmed: 30028513
Biophys J. 1999 Jun;76(6):2879-86
pubmed: 10354416
Structure. 2006 Jan;14(1):159-66
pubmed: 16407075
Acta Crystallogr D Struct Biol. 2018 Feb 1;74(Pt 2):143-151
pubmed: 29533240
Acta Crystallogr D Struct Biol. 2018 Feb 1;74(Pt 2):68-84
pubmed: 29533233
Protein Sci. 2004 Nov;13(11):3051-5
pubmed: 15459339
Semin Cell Dev Biol. 2018 May;77:33-42
pubmed: 28890419
PLoS One. 2011;6(9):e24365
pubmed: 21909430
Acta Crystallogr D Biol Crystallogr. 2005 Apr;61(Pt 4):449-57
pubmed: 15805600
Nature. 1985 Jul 11-17;316(6024):170-4
pubmed: 4010788
Exp Brain Res. 2010 Jan;200(2):125-40
pubmed: 19690847
Nature. 2016 Aug 4;536(7614):108-11
pubmed: 27368053
Neuron. 2006 Nov 9;52(3):475-84
pubmed: 17088213
Virus Res. 2017 Jan 2;227:115-123
pubmed: 27751882
Eur J Neurosci. 2020 Sep 5;:
pubmed: 32888346
Protein Sci. 2011 May;20(5):908-24
pubmed: 21416545
Antiviral Res. 2014 Mar;103:39-50
pubmed: 24418573
Biol Psychiatry. 2017 Apr 1;81(7):573-584
pubmed: 27567310
Science. 2017 Aug 11;357(6351):609-612
pubmed: 28798133
Proc Natl Acad Sci U S A. 2007 Apr 24;104(17):7062-7
pubmed: 17438295
Nat Rev Mol Cell Biol. 2015 Mar;16(3):178-89
pubmed: 25650798
Acta Crystallogr D Biol Crystallogr. 2010 Apr;66(Pt 4):486-501
pubmed: 20383002
Cell Mol Life Sci. 2015 Nov;72(21):4193-203
pubmed: 26012696
Science. 2015 Jul 3;349(6243):95-8
pubmed: 26044299
Proc Natl Acad Sci U S A. 2019 May 14;116(20):10048-10057
pubmed: 31036670
J Physiol. 2011 Dec 15;589(Pt 24):5909-10
pubmed: 22174138
Cell. 2009 Nov 13;139(4):780-90
pubmed: 19914170
Acta Crystallogr D Biol Crystallogr. 2012 Apr;68(Pt 4):328-35
pubmed: 22505253
Acta Crystallogr D Biol Crystallogr. 2012 Apr;68(Pt 4):352-67
pubmed: 22505256
Biophys J. 2001 Jun;80(6):2946-53
pubmed: 11371467
Mol Cell. 2005 Jan 7;17(1):137-43
pubmed: 15629724
Neuron. 2006 Nov 9;52(3):461-74
pubmed: 17088212
Nat Neurosci. 2011 Mar;14(3):279-84
pubmed: 21278731
Nucleic Acids Res. 2019 Jul 2;47(W1):W636-W641
pubmed: 30976793
Acta Crystallogr D Biol Crystallogr. 2010 Feb;66(Pt 2):125-32
pubmed: 20124692
Nucleic Acids Res. 2018 Jul 2;46(W1):W296-W303
pubmed: 29788355
Cell. 2018 Mar 22;173(1):275
pubmed: 29570995
Curr Opin Genet Dev. 2014 Apr;25:8-14,138
pubmed: 24463272
Nat Neurosci. 2020 Feb;23(2):172-175
pubmed: 31907439
Sci Adv. 2020 Jan 01;6(1):eaay6354
pubmed: 31911950
J Neurosci. 2007 Sep 26;27(39):10445-55
pubmed: 17898216
Nature. 2013 May 30;497(7451):643-6
pubmed: 23719463
Nucleic Acids Res. 1997 Sep 1;25(17):3389-402
pubmed: 9254694
Neuron. 2006 Nov 9;52(3):445-59
pubmed: 17088211
eNeuro. 2014 Nov 12;1(1):
pubmed: 26464963
Proc Natl Acad Sci U S A. 2004 Mar 9;101(10):3414-9
pubmed: 14993605
J Comput Chem. 2011 May;32(7):1488-91
pubmed: 21425296
Neuron. 2015 Apr 22;86(2):490-500
pubmed: 25864631
Cell. 2018 Jan 11;172(1-2):262-274.e11
pubmed: 29328915
Nature. 1996 Mar 28;380(6572):316-22
pubmed: 8598927
Protein Sci. 2018 Jan;27(1):129-134
pubmed: 28875543
FEBS J. 2021 May;288(9):2930-2955
pubmed: 33175445
J Biol Chem. 2014 May 16;289(20):14121-31
pubmed: 24675079
Nat Struct Mol Biol. 2020 Nov;27(11):1024-1031
pubmed: 32929280
J Appl Crystallogr. 2007 Aug 1;40(Pt 4):658-674
pubmed: 19461840
Acta Crystallogr A. 2008 Jan;64(Pt 1):112-22
pubmed: 18156677
Acta Crystallogr D Biol Crystallogr. 2010 Apr;66(Pt 4):470-8
pubmed: 20383000
Biochemistry. 2008 Feb 26;47(8):2289-97
pubmed: 18220423
Mol Cell. 2019 Jul 11;75(1):13-25.e5
pubmed: 31151856
Mol Cell. 2006 Dec 8;24(5):677-688
pubmed: 17157251
J Mol Biol. 1990 Oct 5;215(3):403-10
pubmed: 2231712
Acta Crystallogr D Biol Crystallogr. 2012 Dec;68(Pt 12):1622-31
pubmed: 23151627
Structure. 2004 Jul;12(7):1157-63
pubmed: 15242592
Proc Natl Acad Sci U S A. 2007 Mar 13;104(11):4353-8
pubmed: 17360528
Mol Cell. 2014 Sep 18;55(6):938-946
pubmed: 25175025
Proteins. 2012 Jul;80(7):1715-35
pubmed: 22411565
Proc Natl Acad Sci U S A. 1998 Nov 10;95(23):13419-24
pubmed: 9811815