The rice RNase P protein subunit Rpp30 confers broad-spectrum resistance to fungal and bacterial pathogens.

Ascomycota Gene Expression Regulation, Plant Magnaporthe Oryza / genetics Plant Diseases / genetics Plant Proteins / genetics Protein Subunits Ribonuclease P Xanthomonas
HDT701 OsRpp30 Pyricularia oryzae (syn. Magnaporthe oryzae) RNase P Xanthomonas oryzae pv. oryzae

Journal

Plant biotechnology journal
ISSN: 1467-7652
Titre abrégé: Plant Biotechnol J
Pays: England
ID NLM: 101201889

Informations de publication

Date de publication:
10 2021
Historique:
revised: 25 03 2021
received: 11 12 2020
accepted: 25 04 2021
pubmed: 2 5 2021
medline: 26 10 2021
entrez: 1 5 2021
Statut: ppublish

Résumé

RNase P functions either as a catalytic ribonucleoprotein (RNP) or as an RNA-free polypeptide to catalyse RNA processing, primarily tRNA 5' maturation. To the growing evidence of non-canonical roles for RNase P RNP subunits including regulation of chromatin structure and function, we add here a role for the rice RNase P Rpp30 in innate immunity. This protein (encoded by LOC_Os11g01074) was uncovered as the top hit in yeast two-hybrid assays performed with the rice histone deacetylase HDT701 as bait. We showed that HDT701 and OsRpp30 are localized to the rice nucleus, OsRpp30 expression increased post-infection by Pyricularia oryzae (syn. Magnaporthe oryzae), and OsRpp30 deacetylation coincided with HDT701 overexpression in vivo. Overexpression of OsRpp30 in transgenic rice increased expression of defence genes and generation of reactive oxygen species after pathogen-associated molecular pattern elicitor treatment, outcomes that culminated in resistance to a fungal (P. oryzae) and a bacterial (Xanthomonas oryzae pv. oryzae) pathogen. Knockout of OsRpp30 yielded the opposite phenotypes. Moreover, HA-tagged OsRpp30 co-purified with RNase P pre-tRNA cleavage activity. Interestingly, OsRpp30 is conserved in grass crops, including a near-identical C-terminal tail that is essential for HDT701 binding and defence regulation. Overall, our results suggest that OsRpp30 plays an important role in rice immune response to pathogens and provides a new approach to generate broad-spectrum disease-resistant rice cultivars.

Identifiants

DOI: 10.1111/pbi.13612 PMID: 33932077 PMC: PMC8486239
pubmed: 33932077
doi: 10.1111/pbi.13612
pmc: PMC8486239
doi:

Substances chimiques

Plant Proteins 0
Protein Subunits 0
Ribonuclease P EC 3.1.26.5

Types de publication

Journal Article Research Support, Non-U.S. Gov't

Langues

eng

Sous-ensembles de citation

IM

Pagination

1988-1999

Subventions

Organisme : NIH HHS
ID : S10 OD023582
Pays : United States

Informations de copyright

© 2021 The Authors. Plant Biotechnology Journal published by Society for Experimental Biology and The Association of Applied Biologists and John Wiley & Sons Ltd.

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Auteurs

Wei Li (W)

State Key Laboratory for Biology of Plant Diseases and Insect Pests, Institute of Plant Protection, Chinese Academy of Agricultural Sciences, Beijing, China.
Hunan Provincial Key Laboratory for Biology and Control of Plant Diseases and Insect Pests and College of Plant Protection, Hunan Agricultural University, Changsha, Hunan, China.
Department of Plant Pathology, The Ohio State University, Columbus, OH, USA.

Yehui Xiong (Y)

State Key Laboratory for Biology of Plant Diseases and Insect Pests, Institute of Plant Protection, Chinese Academy of Agricultural Sciences, Beijing, China.

Lien B Lai (LB)

Department of Chemistry and Biochemistry, Center for RNA Biology, The Ohio State University, Columbus, Ohio, USA.

Kai Zhang (K)

State Key Laboratory for Biology of Plant Diseases and Insect Pests, Institute of Plant Protection, Chinese Academy of Agricultural Sciences, Beijing, China.

Zhiqiang Li (Z)

State Key Laboratory for Biology of Plant Diseases and Insect Pests, Institute of Plant Protection, Chinese Academy of Agricultural Sciences, Beijing, China.

Houxiang Kang (H)

State Key Laboratory for Biology of Plant Diseases and Insect Pests, Institute of Plant Protection, Chinese Academy of Agricultural Sciences, Beijing, China.

Liangying Dai (L)

Hunan Provincial Key Laboratory for Biology and Control of Plant Diseases and Insect Pests and College of Plant Protection, Hunan Agricultural University, Changsha, Hunan, China.

Venkat Gopalan (V)

Department of Chemistry and Biochemistry, Center for RNA Biology, The Ohio State University, Columbus, Ohio, USA.

Guo-Liang Wang (GL)

Department of Plant Pathology, The Ohio State University, Columbus, OH, USA.
ORCID: 0000-0001-8877-3064

Wende Liu (W)

State Key Laboratory for Biology of Plant Diseases and Insect Pests, Institute of Plant Protection, Chinese Academy of Agricultural Sciences, Beijing, China.
ORCID: 0000-0002-5570-1395

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Classifications MeSH

Recherchemedicale.com › Eucaryotes › Champignons › Ascomycota › The rice RNase P protein subunit Rpp30 confers...
Recherchemedicale.com › Phénomènes génétiques › Régulation de l'expression des gènes › Régulation de l'expression des gènes végétaux › The rice RNase P protein subunit Rpp30 confers...
Recherchemedicale.com › Eucaryotes › Champignons › Ascomycota › Magnaporthe › The rice RNase P protein subunit Rpp30 confers...
Recherchemedicale.com › Eucaryotes › Viridiplantae › Streptophyta › Embryophyta › Tracheobionta › Magnoliopsida › Poaceae › Oryza › The rice RNase P protein subunit Rpp30 confers...
Recherchemedicale.com › Phénomènes physiologiques des plantes › Maladies des plantes › The rice RNase P protein subunit Rpp30 confers...
Recherchemedicale.com › Acides aminés, peptides et protéines › Protéines › Protéines végétales › The rice RNase P protein subunit Rpp30 confers...
Recherchemedicale.com › Acides aminés, peptides et protéines › Protéines › Sous-unités de protéines › The rice RNase P protein subunit Rpp30 confers...
Recherchemedicale.com › Acides aminés, peptides et protéines › Protéines › Nucléoprotéines › Protéines de liaison à l'ARN › Ribonucléoprotéines › Ribonuclease P › The rice RNase P protein subunit Rpp30 confers...
Recherchemedicale.com › Bactéries › Proteobacteria › Gammaproteobacteria › Xanthomonadaceae › Xanthomonas › The rice RNase P protein subunit Rpp30 confers...